Impact of priming on seed germination, seedling growth and gene expression in common vetch under salinity stress

Abstract

Salinity is one of the most important abiotic stress factors that is expanding its influence because of global climate change and global warming. It causes gene expression changes, a reduction in seed germination and related characteristics, and poor seedling establishment in many crop plants by creating a lower osmotic potential in the seedbed and/or toxic ion effects in germinated seeds. In recent years, seed priming has been considered a promising strategy in modern stress management to protect plants against stress conditions. This study was conducted to elucidate the effects of osmopriming with polyethylene glycol 6000 (PEG-6000) on seed germination, seedling growth and gene expression in the common vetch (Vicia sativa L.) in different saline conditions. Common vetch seeds were primed with PEG-6000 solutions having different osmotic potentials (0.00, -0.50, -0.75, -1.00, -1.25, and -1.50 MPa) for 12 hours. Control (un-primed) and primed seeds were germinated and seedlings were grown in different saline conditions (EC= zero, 4, 8 and 16 dS m -1 ). Furthermore, gene expression was compared in the primed seedlings in two different osmotic potentials (0.00 and -1.50 MPa) by microarray technology. Results demonstrated that germination percentage of common vetch seeds and seedling growth were diminished by high salinity. However, several priming treatments alleviated the adverse effects of high salinity on germination and early seedling growth of common vetch. The microarray showed that the expression of many genes in both stress and normal conditions was not significantly different.

The efficacy of different seed priming agents for promoting sorghum germination under salt stress

“…Similarly, Abraha et al [ 42 ] showed that seed priming with 5g/L NaCl significantly improved early seedling growth under salt stress in maize. Likewise, seed priming with PEG-6000 alleviated the adverse effects of salt stress on seed germination and early seedling growth in common vetch [ 43 ]. Seed priming with distilled water increased the germination index, root and shoot length, and dry weight and promoted the germination and normal seedling growth of maize under salt stress [ 44 ].…”

The Impact of Glucose-induced Priming on Nutrients Accumulation and Certain Primary Attributes of Brassica napus L. Under the Saline Regimes

“…There is sufficient evidence that priming induces seeds to absorb more water and helps in increasing percent emergence (Atak et al, 2006;Kaya et al, 2006). The reason behind the negative impact of salt stress on seedling emergence is accredited to the physiological drought it creates or the ion toxicity on the germinated seeds (Aydinoglu et al, 2019).…”

“…The previous studies on wheat (Afzal et al, 2005;Abbas et al, 2018;Ehtaiwesh and Almajdor, 2021), canola (Hassanpoughdam et al, 2009;Heshmat et al, 2011;Aboutalebian et al, 2012;Zhu et al, 2021), Calendula officinalis (Sedghi et al, 2010), alfaafa (Jorjandi & Sirchi, 2012), Calotropis procera (Taghvaei et al, 2012), soyabean (Ahmadvand et al, 2012), Aeluropus (Nejad et al, 2013), cumin (Shoor et al, 2014, maize (Akter et al, 2018;Shah et al, 2021), Vicia (Aydinoglu et al, 2019), Camilina (Huang et al, 2021) and hargel (Salih et al, 2022) have confirmed stimulatory effects of pre-soaking amendments on plumule lengths under salt stress which fully support our findings. However, some workers have reported a decrease in the plumule growth of plants like wheat (Akbarimoghaddam et al, 2011;Biabani et al, 2013;Khan et al, 2018) and canola (Bybordi and Tabatabaei, 2009;Mohammadi et al, 2010) 1.5787 a Alpha = 0.05 %.…”

“…The previous workers have confirmed stimulatory tendencies of pre-soaking amendments on various traits of plants like Triticum (Iqbal & Ashraf, 2007), Helianthus (Kaya et al, 2006), chickpea (Kaur et al, 2002), Capsicum (Patade et al, 2011), Glysine max (Sadeghi et al, 2011), maize (Foti et al, 2008), cucumber (Ghasemi-Golezani & Esmaeilpour, 2008). The priming tendencies of various chemicals such as Calcium Chloride (Fuller et al, 2012;Taghvaei et al, 2012;Abbas et al, 2018), Sodium Chloride (Jamal et al, 2011;Nejad et al, 2013;Shoor et al, 2014), Zinc Oxide nano-particles (Al-salama 2022), sorghum water (Huang et al, 2021), Potassium Nitrate (Ahmadvand et al, 2012), aspirin (Ehtaiwesh & Almajdob, 2021), Potassium Permangante (Hassanpouraghdam et al, 2009;Hassanpouraghdam et al, 2009), vitamin B12 (Jorjandi & Sirchi, 2012), plant hormones (Sedghi et al, 2010;Salih et al, 2022), PEG-6000 (Aydinoglu et al, 2019;Fuller et al, 2012), Ascorbic Acid, Potassium Silicate, Proline and Spermidine (Feghhenabi et al, 2020) had been already studied. In this study, we have explored the priming tendencies of glucose on a prominant oilyielding plant, the canola for the first time keeping in view the importance of glucose for initial stages of germination of plants.…”

Comparative Transcriptomic Analysis of Root and Leaf Transcript Profiles Reveals the Coordinated Mechanisms in Response to Salinity Stress in Common Vetch

“…When common vetch seedlings were grown under salinity stress conditions, their height and root length decreased; moreover, the seedlings died when subjected to the greatest level of salinity, and the shoot dry weight, leaf number, and leaf area per seedling decreased up to 80%, 61%, and 89%, respectively [ 27 ]. The germination percentage of common vetch seeds and seedling growth also decreased in response to high salinity [ 28 ]. Previous studies in common vetch have focused primarily on the characterization and evaluation of the physiological mechanisms that occur in response to salinity stress; however, the detailed mechanism underlying the response to salinity stress of this important legume forage species at the molecular level have not been elucidated.…”