Impact of reduced incubation times on culture and susceptibility testing of urine cultures incubated in the BD Kiestra ReadA Compact incubators

Desjardins, Marc; Sant, Nadia; Miron-Celis, M.; Gosal, Jasmine; Jémus, Marie-France; Jémus-Gonzalez, E

doi:10.1016/j.diagmicrobio.2019.114899

Cited by 2 publications

(6 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several studies have shown that the ReadA system yields better colony recovery and requires less time to detect growth than conventional incubators. 15, 24, 31 The ReadA module reduced touches per plate by 67% and total HOT by 53% (19.5 minutes) during incubation and plate reading. Reading HOT per specimen was reduced on all protocol types.…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…BD Kiestra InoqulA specimen processor (but InoqulA) automates plate labeling, specimen preparation, and inoculation. [20][21][22][23] BD Kiestra ReadA standalone (but ReadA), automates plate incubation, plate sorting, and high-resolution plate imaging 24 by providing standardized digital image acquisition through the BD Synapsys Informatics Solution (but Synapsys) (version 3.5).…”

Section: Introductionmentioning

confidence: 99%

“…Several studies have shown the benefits of implementing Kiestra automation systems in clinical microbiology laboratories, including shortening workflow time and optimizing result quality. 13,14,18,19,22,[24][25][26] This report describes a step-wise utilization of automated inoculation, incubation, and imaging modules, in a simulated clinical laboratory environment, in order to streamline workflow processes. Here, we recorded each workflow step (plate setup, inoculation, and reading) and timing for plates that were processed manually, compared to the same number and types of plates that were processed via Kiestra automated modules: InoqulA (BD product number: 446973) for inoculation, ReadA (BD product number: 446948) for incubation and imaging, and Synapsys (BD product number: 444158) for digital image reading.…”

Section: Introductionmentioning

confidence: 99%

“…BD Kiestra InoqulA specimen processor ( but InoqulA) automates plate labeling, specimen preparation, and inoculation. 20-23 BD Kiestra ReadA standalone ( but ReadA), automates plate incubation, plate sorting, and high-resolution plate imaging 24 by providing standardized digital image acquisition through the BD Synapsys Informatics Solution ( but Synapsys) (version 3.5). Several studies have shown the benefits of implementing Kiestra automation systems in clinical microbiology laboratories, including shortening workflow time and optimizing result quality.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Automation of plate inoculation and reading reduces process time in the clinical microbiology laboratory, compared to a manual workflow

Peisach

Krotkov

Shaye

et al. 2022

Preprint

View full text Add to dashboard Cite

We evaluated the benefits of automation for clinical microbiology laboratory workflow and compared the time required for culture inoculation and plate reading between manual processes and BD Kiestra (but Kiestra) automation. Kiestra automation consists of different modules, each of which facilitate different tasks and work together from specimen inoculation to results reporting. We tracked the steps and measured the associated times-to-completion for both manual and automated workflows, including number of plate touches, hands-on-time (HOT), total technologist time (TTT), and walk-away time (WAT). Manual and automated processes both included 90 samples, and a total of 180 agar plates. There were three media quantity protocols used to mimic common laboratory samples: 1) 30 urine specimens on one biplate; 2) 30 urine specimens divided across two plates; and 3) 30 blood or wound specimens divided across three plates; all cultures incubated for a minimum of 18 hours prior to reading or imaging. Automation reduced HOT by 85% and overall plate touches by 88%. Automated inoculation (through implementation of the BD InoqulA module) resulted in a 100% reduction in plate touches and created 81.5-minutes of WAT. Automation of culture reading (through implementation of the BD ReadA incubation/plate imaging module and BD Synapsys Informatics) reduced HOT by 53%. Overall, laboratory automation resulted in shorter TTT and created WAT when compared to manual processes. Automation can facilitate increased processing capacity, more efficient use of the labor force, and reduced time to results in the clinical microbiology laboratory.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Automation of plate inoculation and reading reduces process time in the clinical microbiology laboratory, compared to a manual workflow

Peisach

Krotkov

Shaye

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

Comparison of an automated DNA extraction and 16S rDNA real time PCR/sequencing diagnostic method using optimized reagents with culture during a 15-month study using specimens from sterile body sites

Egli

Risch

et al. 2022

BMC Microbiol

View full text Add to dashboard Cite

Background 16S rDNA-PCR for the identification of a bacterial species is an established method. However, the DNA extraction reagents as well as the PCR reagents may contain residual bacterial DNA, which consequently generates false-positive PCR results. Additionally, previously used methods are frequently time-consuming. Here, we describe the results obtained with a new technology that uses DNA-free reagents for automated DNA extraction and subsequent real time PCR using sterile clinical specimens. Results In total, we compared 803 clinical specimens using real time PCR and culturing. The clinical specimens were mainly of orthopedic origin received at our diagnostic laboratory. In 595 (74.1%) samples, the results were concordant negative, and in 102 (12.7%) the results were concordant positive. A total of 170 (21.2%) clinical specimens were PCR-positive, of which 62 (36.5% from PCR positive, 7.7% in total) gave an additional benefit to the patient since only the PCR result was positive. Many of these 62 positive specimens were strongly positive based on crossingpoint values (54% < Cp 30), and these 62 positive clinical specimens were diagnosed as medically relevant as well. Thirty-eight (4.2%) clinical specimens were culture-positive (25 of them were only enrichment culture positive) but PCR-negative, mainly for S. epidermidis, S. aureus and C. acnes. The turnaround times for negative specimens were 4 hours (automated DNA extraction and real time PCR) and 1 working day for positive specimens (including Sanger sequencing). Melting-curve analysis of SYBR Green-PCR enables the differentiation of specific and unspecific PCR products. Using Ripseq, even mixed infections of 2 bacterial species could be resolved. Conclusions For endocarditis cases, the added benefit of PCR is obvious. The crucial innovations of the technology enable timely reporting of explicit reliable results for adequate treatment of patients. Clinical specimens with truly PCR-positive but culture-negative results represent an additional benefit for patients. Very few results at the detection limit still have to be critically examined.

show abstract

Impact of reduced incubation times on culture and susceptibility testing of urine cultures incubated in the BD Kiestra ReadA Compact incubators

Cited by 2 publications

References 13 publications

Automation of plate inoculation and reading reduces process time in the clinical microbiology laboratory, compared to a manual workflow

Automation of plate inoculation and reading reduces process time in the clinical microbiology laboratory, compared to a manual workflow

Comparison of an automated DNA extraction and 16S rDNA real time PCR/sequencing diagnostic method using optimized reagents with culture during a 15-month study using specimens from sterile body sites

Contact Info

Product

Resources

About