Impact of remnant healthy pulp and apical tissue on outcomes after simulated regenerative endodontic procedure in rat molars

Edanami, Naoki; Yoshiba, Kunihiko; Shirakashi, Mari; Belal, Razi Saifullah Ibn; Yoshiba, Nagako; Ohkura, Naoto; Tohma, Aiko; Takeuchi, Ryosuke; Okiji, Takashi; Noiri, Yuichiro

doi:10.1038/s41598-020-78022-w

Cited by 8 publications

(7 citation statements)

References 44 publications

(54 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, non-collagenous proteins in apical connective tissue promote or inhibit calcium salt precipitation [ 39 ]. In future studies, the calcium salt-forming ability of Bio-C Temp should be evaluated under conditions that resemble clinical settings, closely using root canal treatment models in rats [ 40 , 41 ] or dogs [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

In Vivo Assessment of the Calcium Salt-Forming Ability of a New Calcium Silicate-Based Intracanal Medicament: Bio-C Temp

et al. 2023

Self Cite

View full text Add to dashboard Cite

Calcium salt precipitation induced by intracanal medicaments contributes to the formation of apical hard tissue during apexification. This study compared the calcium salt-forming ability of a new calcium silicate-based intracanal medicament (Bio-C Temp) with that of two commercial calcium hydroxide pastes (Calcipex Plane II and Vitapex) in a rat subcutaneous implantation model. Polytetrafluoroethylene tubes containing each of the three materials were subcutaneously implanted in 4-week-old male Wistar rats. After 28 days, the composition and amount of calcium salts formed at the material–tissue interface were assessed using micro-Raman spectroscopy, X-ray diffraction, and elemental mapping. The tested materials produced white precipitates that had Raman spectra with peaks corresponding to hydroxyapatite and calcite. X-ray diffraction detected hydroxyapatite formation on Calcipex Plane II and Vitapex implants, as well as calcite formation on all three materials. Elemental mapping revealed that Bio-C Temp generated significantly smaller calcium- and phosphorus-rich calcified regions within the subcutaneous connective tissue than Vitapex. These results indicate that Bio-C Temp produced less calcium salt in rat subcutaneous tissue than Vitapex, although all materials formed hydroxyapatite and calcite in rat subcutaneous tissue. Bio-C Temp could be less effective than Vitapex in promoting apical hard tissue formation during apexification.

show abstract

Section: Discussionmentioning

confidence: 99%

In Vivo Assessment of the Calcium Salt-Forming Ability of a New Calcium Silicate-Based Intracanal Medicament: Bio-C Temp

et al. 2023

Self Cite

View full text Add to dashboard Cite

show abstract

“…In clinical settings, HCSCs contact dental pulp or periodontal tissue. Therefore, future studies should evaluate the in vivo apatite-forming ability of HCSCs under conditions that more closely mimic clinical settings using pulp capping models [41,42] or root canal lling models [43][44][45]. However, hydroxyapatite formation is due to the chemical reactions between HCSCs and body uids; thus, we assume that hydroxyapatite-forming behaviors of HCSCs in vivo do not depend on the types of tissue the materials contact.…”

Section: Discussionmentioning

confidence: 99%

In Vivo Assessment of the Apatite-Forming Ability of Second-Generation Hydraulic Calcium Silicate Cements Using a Rat Subcutaneous Implantation Model

Edanami

Takenaka

Belal

et al. 2023

Preprint

View full text Add to dashboard Cite

Hydroxyapatite formation on endodontic hydraulic calcium silicate cements (HCSCs) plays a significant role in sealing the root canal system and elevating the hard-tissue inductivity of the materials. This study aimed to evaluate the in vivo apatite-forming ability of 13 second-generation HCSCs using a representative first-generation HCSC (white ProRoot MTA: PR) as a positive control. Thirteen second-generation HCSCs and PR were loaded into polytetrafluoroethylene tubes and implanted in subcutaneous tissue of 4-week-old male Wistar rats. At 28 days after implantation, hydroxyapatite formation on the HCSC implants was assessed with micro-Raman spectroscopy, surface ultrastructural and elemental characterization, and elemental mapping of the material–tissue interface. A Raman band for hydroxyapatite (v1 PO43- band at 960 cm−t) and hydroxyapatite-like calcium-phosphorus-rich spherical precipitates were detected on six second-generation HCSCs and PR. In the elemental mapping, calcium-phosphorus-rich hydroxyapatite-layer-like regions were not observed on the seven HCSCs that showed neither the hydroxyapatite Raman band nor hydroxyapatite-like spherical precipitates. These results indicated that only 6 of the 13 second-generation HCSCs produced a detectable amount of hydroxyapatite in rat subcutaneous tissue within 28 days, similar to PR. The seven second-generation HCSCs that did not exhibit hydroxyapatite formation may not be suitable alternatives to PR due to their weak in vivo apatite-forming ability.

show abstract

“…In clinical use, the HCSCs contact dental pulp or periapical tissue. Therefore, future studies should evaluate the in vivo apatite-forming ability of HCSCs in clinically relevant tissues such as pulp tissue [62,63] or periapical tissue [13,64,65].…”

Section: Discussionmentioning

confidence: 99%

In Vivo Assessment of the Apatite-Forming Ability of New-Generation Hydraulic Calcium Silicate Cements Using a Rat Subcutaneous Implantation Model

Edanami

Takenaka

Belal

et al. 2023

JFB

Self Cite

View full text Add to dashboard Cite

Hydroxyapatite formation on endodontic hydraulic calcium silicate cements (HCSCs) plays a significant role in sealing the root canal system and elevating the hard-tissue inductivity of the materials. This study evaluated the in vivo apatite-forming ability of 13 new-generation HCSCs using an original HCSC (white ProRoot MTA: PR) as a positive control. The HCSCs were loaded into polytetrafluoroethylene tubes and implanted in the subcutaneous tissue of 4-week-old male Wistar rats. At 28 days after implantation, hydroxyapatite formation on the HCSC implants was assessed with micro-Raman spectroscopy, surface ultrastructural and elemental characterization, and elemental mapping of the material–tissue interface. Seven new-generation HCSCs and PR had a Raman band for hydroxyapatite (v1 PO43− band at 960 cm−1) and hydroxyapatite-like calcium-phosphorus-rich spherical precipitates on the surfaces. The other six HCSCs with neither the hydroxyapatite Raman band nor hydroxyapatite-like spherical precipitates did not show calcium-phosphorus-rich hydroxyapatite-layer-like regions in the elemental mapping. These results indicated that 6 of the 13 new-generation HCSCs possessed little or no ability to produce hydroxyapatite in vivo, unlike PR. The weak in vivo apatite-forming ability of the six HCSCs may have a negative impact on their clinical performance.

show abstract

Impact of remnant healthy pulp and apical tissue on outcomes after simulated regenerative endodontic procedure in rat molars

Cited by 8 publications

References 44 publications

In Vivo Assessment of the Calcium Salt-Forming Ability of a New Calcium Silicate-Based Intracanal Medicament: Bio-C Temp

In Vivo Assessment of the Calcium Salt-Forming Ability of a New Calcium Silicate-Based Intracanal Medicament: Bio-C Temp

In Vivo Assessment of the Apatite-Forming Ability of Second-Generation Hydraulic Calcium Silicate Cements Using a Rat Subcutaneous Implantation Model

In Vivo Assessment of the Apatite-Forming Ability of New-Generation Hydraulic Calcium Silicate Cements Using a Rat Subcutaneous Implantation Model

Contact Info

Product

Resources

About