Impact of sleep deprivation and high-fat feeding on insulin sensitivity and beta cell function in dogs

Abstract

Aims/hypothesis-Insufficient sleep is increasingly recognised as a major risk factor for the development of obesity and diabetes, and short-term sleep loss in clinical studies leads to a reduction in insulin sensitivity. Sleep loss-induced metabolic impairments are clinically relevant, since reductions in insulin sensitivity after sleep loss are comparable to insulin sensitivity differences between healthy individuals and those with impaired glucose tolerance. However, the relative effects of sleep loss vs high-fat feeding in the same individual have not been assessed. In addition, to our knowledge no diurnal (active during the daytime) non-human mammalian model of sleep loss-induced metabolic impairment exists, which limits our ability to study links between sleep and metabolism.Methods-This study examined the effects of one night of total sleep deprivation on insulin sensitivity and beta cell function, as assessed by an IVGTT, before and after 9 months of high-fat feeding in a canine model.Results-One night of total sleep deprivation in lean dogs impaired insulin sensitivity to a similar degree as a chronic high-fat diet (HFD)(normal sleep: 4.95 ± 0.45 mU −1 l −1 min −1 ; sleep deprivation: 3.14 ± 0.21 mU −1 l −1 min −1 ; HFD: 3.74 ± 0.48 mU −1 l −1 min −1 ; mean ± SEM). Hyperinsulinaemic compensation was induced by the chronic HFD, suggesting adequate beta cell response to high-fat feeding. In contrast, there was no beta cell compensation after one night of Josiane L. Broussard Josiane.broussard@colostate.edu. Contribution statement AB and JLB drafted the manuscript and carried out analyses of data. JLB, CMK and RNB designed the study. JLB, IAB, RLP and CMK were involved in acquisition and analysis of data. All authors were involved in writing the paper and had final approval of the manuscript. JLB is the guarantor of this work.

A comparative study of the effect of a gentle ketogenic diet containing medium-chain or long-chain triglycerides on chronic sleep deprivation-induced cognitive deficiency

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Dapagliflozin prevents abdominal visceral and subcutaneous adipose tissue dysfunction in the insulin‐resistant canine model

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