Impact of Slight Change in Water Potential of Culture Media on In vitro Shoot Multiplication and Esterase and Protein Patterns of Simmondsia chinensis L.

Abstract

MPLIFICATION of DNA of 10 jojoba shrubs using 7 RAPD primers indicated that seed propagation of jojoba results in high genetic variation. To produce true-to-type clones, we applied a micropropagation technique. Medium (MS) containing relatively low concentrations (1 or 2mg/L) of 6-Benzylaminopurine (BAP) did not stimulate shoot formation on jojoba nodal explants. The best shoot cloning on nodal explants was established on MS medium containing 3mg/L BAP and 0.1mg/L 1-Naphthaleneacetic acid (NAA). Decrease in water potential (Ψ) of medium after adding 0.5mg/L of mannitol slightly increased the number of formed shoots and further decreased the medium Ψ by increasing NaCl or mannitol concentration, resulting in retardation of shoot formation. Data indicated that shoot formation was more sensitive than shoot growth to changes in Ψ of the medium. Callus weight decreased with decreasing medium Ψ to less than −435.014 MPa using 1g/L of NaCl, while mannitol application to decrease Ψ of the medium up to 435.054 MPa (4g/L) significantly increased callus fresh weight. Jojoba calli clearly expressed the effects of reduced Ψ in MS medium on esterase and protein patterns.

Application of Tissue Culture Techniques to Improve the Productivity of Medicinal Secondary Products from Medicinal Plants

“…For example, while sulfate and ammonium nitrate ions increased the colchicine content of Gloriosa superba callus, a higher concentration of phosphate and calcium decreased alkaloid biosynthesis [67]. The differences in the composition of various PTC media formulations affect on water potential of the cultural environment [68]. Then, different media exerted different values of water potential.…”

“…To enhance the biomass and biosynthesize of secondary metabolites, sucrose is widely used as a carbon source and it was better than maltose, glucose, and others [77]. During in vitro propagation, the optimal concentration of sucrose depends on plant species [15,47,68,73]. For example, feeding the culture medium with 60 mM nitrogen and rise sucrose concentration from 3% sucrose to 5% increase the biomass production and camptothecin accumulation by 2.4-fold in the cell suspension cultures of Nicotiana nimmoniana [77].…”

Effects of chitosan on growth and physiological changes associated adaptation of in vitro obtained banana plantlets to ex vitro conditions

“…Under in vitro culture conditions, regenerants are formed and grown under minimal abiotic stresses on a medium containing abundant nutrients and high humidity within culture vessel (Hassanein et al, 2022). Upon transferring of the in vitro obtained plantlets to ex vitro conditions, they expose to abiotic factors such as altered temperature, light intensity and humidity as well as biotic factors such as soil microflora.…”