Impact of solar Ultraviolet-B on the proteome in soybean lines differing in flavonoid contents

Abstract

Two-dimensional polyacrylamide gel electrophoresis (2-D PAGE) was used to systematically investigate the impact of solar ultraviolet-B (UV-B) radiation on the soybean leaf proteome. In order to investigate the protective role of flavonoids against UV-B, two isolines of the Clark cultivar (the standard line with moderate levels of flavonoids and the magenta line with reduced flavonoids) were grown in the field with or without natural levels of UV-B. The 12-day-old first trifoliates were harvested for proteomic analysis. More than 300 protein spots were reproducibly resolved and detected on each gel. Statistical analysis showed that 67 protein spots were significantly (P < 0.05) affected by solar UV-B. Many more spots were altered by UV-B in the magenta line than in the standard line. Another 12 protein spots were not altered by UV-B but showed significantly (P < 0.05) different accumulations between the two lines, and for most spots the line-specific differences were also observed under UV-B exclusion. Most of the differentially accumulated spots were identified by mass spectrometry. The proteins were quite diverse, and were involved in metabolism, energy, protein destination/storage, protein synthesis, disease/defense, transcription, and secondary metabolism. The results suggest that high levels of flavonoids lead to a reduction in UV-B sensitivity at the proteomic level. Published by Elsevier Ltd.

Salt-induced root protein profile changes in seedlings of maize inbred lines with differing salt tolerances

“…Our observations suggest that this enzyme may increase lignin amounts by lignification, promoting thereby strong seedling growth and resistance to salt stress-induced injuries. Flavonoids are involved in a vast array of plant biological functions associated with defenses against biotic and abiotic stress (Harborne and Williams, 2000;Winkel-Shirley, 2002;Taylor and Grotewold, 2005;Lepiniec et al, 2006;Pourcel et al, 2007;Xu et al, 2008). The abundance of one key flavonoid pathway enzyme, chalcone isomerase (CHI; protein spot C9), was downregulated under salinity stress in our experiment in saltsensitive 'D340', but remained unchanged (p > 0.05) in seedling roots of 'N1145'.…”

Responses of Dynamic Proteome Changes in Zizania Latifolia to Fresh-Cut Operation during Room Temperature (25 °C) Storage

“…34 Of the eleven stress response and defense proteins up-regulated by fresh-cut operation, eight were related to disease resistance. plant basic secretory protein (BSP, spot 2) is believed to be part of the plants defense mechanism against pathogens, 35 β-1,3-glucanase precursor (spot 3, 5), glucan endo-1,3-beta-glucosidase (spot 4) and β-1,3-glucanase (spot 28) could degrade fungal cell wall polysaccharides, 36 universal stress protein family (USP, spot 12) is a small cytoplasmic bacterial protein whose expression is enhanced when the cell is exposed to stress agents, 13 thaumatin-like protein isoform 2 (TLP, spot 21) plays a roles in host defense and developmental processes, 37 while chitinase class I (spot 34) catalyzes the hydrolysis of the beta-1,4-N-acetyl-D-glucosamine linkages in chitin polymers of fungal cell walls. 38 The higher abundances of these proteins (except chitinase class I) in fresh-cut Z. latifolia when compared to those in control provide a good indication that fresh-cut operation induced elevation in anti-pathogen capability.…”

Advances in Crop Responses to Enhanced Uv-B Radiation

“…We still do not have a complete understanding of the molecular bases of these responses, but they generally are the result of signal perception by receptor molecules and transduction of a response signal to the cellular machinery, a part of which may regulate gene expression. However, Xu et al (2008b) did not detect protein effects involved in the signal transduction, because many of the proteins involved in the signal transduction occurred in too low abundance in crude extracts and membrane proteins were usually under-represented on 2-D PAGE gels. Also research at the mRNA level may not necessarily translate into the quantity and quality of the final gene products, i.e.…”