Impact of sterilization methods on electrospun scaffolds for tissue engineering

Rediguieri, Carolina Fracalossi; Sassonia, Rogério Côrte; Dua, Kamal; Kikuchi, Irene Satiko; Pinto, Terezinha de Jesus Andreoli

doi:10.1016/j.eurpolymj.2016.07.016

Cited by 55 publications

(63 citation statements)

References 60 publications

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“…No significant change was seen in the drug release profile after sterilization ( Figure 5). The effects of the sterilization observed in this work are in good agreement with the literature [13,14]. Hence, selecting gamma irradiation (10-15 kGy) as the sterilization method for the rest of the study was a good compromise.…”

Section: Selection Of the Sterilization Methodssupporting

confidence: 88%

“…PLC contains about 30% of PCL and 70% of PLLA. Moreover, ethylene oxide sterilization could induce a risk of toxicity due to residual ethylene oxide confined in the polymer [14].…”

Section: Selection Of the Sterilization Methodsmentioning

confidence: 99%

“…The sterilization has to be investigated in the first developmental steps; it can significantly change the scaffold properties [13,14]. This critical process can decrease the mechanical properties, which are required to ensure the handling and the setup of the membrane.…”

Section: Membrane Formulation Designmentioning

confidence: 99%

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Electrospun Bioresorbable Membrane Eluting Chlorhexidine for Dental Implants

et al. 2020

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To prevent the uncontrolled development of a pathogenic biofilm around a dental implant, an antimicrobial drug-release electrospun membrane, set up between the implant and the gingival tissue, was developed by taking several technical, industrial and regulatory specifications into account. The membrane formulation is made of a blend of poly(l-lactic–co–gycolic acid) (PLGA, 85:15) and poly(l-lactic acide–co–ɛ-caprolactone) (PLC, 70:30) copolymers with chlorhexidine diacetate (CHX) complexed with β-cyclodextrin (CD). The amount of residual solvent, the mechanical properties and the drug release kinetics were tuned by the copolymers’ ratio, between 30% and 100% of PLC, and a CHX loading up to 20% w/w. The membranes were sterilized by γ-irradiation without significant property changes. The fiber′s diameter was between 600 nm and 3 µm, depending on the membrane composition and the electrospinning parameters. CHX was released in vitro over 10 days and the bacterial inhibitory concentration, 80 µg·mL−1, was reached within eight days. The optimal membrane, PGLA/PLC/CHX-CD (60%/40%/4%), exhibited a breaking strain of 50%, allowing its safe handling. This membrane and a membrane without CHX-CD were implanted subcutaneous in a rat model. The cell penetration remained low. The next step will be to increase the porosity of the membrane to improve the dynamic cell penetration and tissue remodeling.

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Section: Selection Of the Sterilization Methodssupporting

confidence: 88%

“…PLC contains about 30% of PCL and 70% of PLLA. Moreover, ethylene oxide sterilization could induce a risk of toxicity due to residual ethylene oxide confined in the polymer [14].…”

Section: Selection Of the Sterilization Methodsmentioning

confidence: 99%

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Electrospun Bioresorbable Membrane Eluting Chlorhexidine for Dental Implants

et al. 2020

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“…We chose 75% ethanol to sterilize all the scaffolds in this study. Previous studies have proved that treatment with 75% ethanol for 1 h has no alterations on the morphology and hydrophilicity of PCL scaffolds [24]. We did not observe any obvious cytotoxicity from the PCL material in our confocal microscopy and CCK-8 assay analyses.…”

Section: Cytotoxicity Of Pcl and Cell Adhesion Proliferation Oncontrasting

confidence: 48%

Fabrication and Application of a 3D-Printed Poly-ε-Caprolactone Cage Scaffold for Bone Tissue Engineering

Wang

et al. 2020

BioMed Research International

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Poly-ε-caprolactone (PCL) is a promising synthetic material in bone tissue engineering (BTE). Particularly, the introduction of rapid prototyping (RP) represents the possibility of manufacturing PCL scaffolds with customized appearances and structures. Bio-Oss is a natural bone mineral matrix with significant osteogenic effects; however, it has limitations in being constructed and maintained into specific shapes and sites. In this study, we used RP and fabricated a hollow-structured cage-shaped PCL scaffold loaded with Bio-Oss to form a hybrid scaffold for BTE. Moreover, we adopted NaOH surface treatment to improve PCL hydrophilicity and enhance cell adhesion. The results showed that the NaOH-treated hybrid scaffold could enhance the osteogenesis of human bone marrow-derived mesenchymal stem cells (hBMMSCs) both in vitro and in vivo. Altogether, we reveal a novel hybrid scaffold that not only possesses osteoinductive function to promote bone formation but can also be fabricated into specific forms. This scaffold design may have great application potential in bone tissue engineering.

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“…Among physical methods for sterilization, heating is very simple. Here, the sterilizing agent is moist or dry heat [259][260][261][262]. In spite of the sterilization by heat being inexpensive and safe, it presents disadvantages regarding the degradation of polymeric biomaterials, since the technique often uses temperatures that exceed the glass transition temperature (T g ) and melting temperature (T m ) of the material [263][264][265].…”

Section: Heatingmentioning

confidence: 99%