Impact of structural stability of cold adapted Candida antarctica lipase B (CaLB): in relation to pH, chemical and thermal denaturation

Rabbani, Gulam; Ahmad, Ejaz; Khan, Rizwan Hasan; Ashraf, Mohammad; Bhat, Rajiv; Khan, Rizwan Hasan

doi:10.1039/c4ra17093h

Cited by 162 publications

(57 citation statements)

References 51 publications

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“…The immobilization values were 35 and 33 mg/g for SBA-15-NH 2 G-PC and SBA-15-NH 2 G-CalB, respectively, similar to those obtained by Bautista et al [4] (36.1 mg/g) and slightly lower than that obtained by Serra et al [30] (44 mg/g), both using CalB as enzyme. The slightly higher incorporation of enzyme in the case of PC may be due to the fact that its size is somewhat smaller (3 × 3.2 × 6.6 nm) [31] and can enter into the pores more easily than CalB (3 × 4 × 6 nm) [32].…”

Section: Characterization Of the Synthesized Enzymatic Catalystmentioning

confidence: 99%

Biodiesel Production (FAEEs) by Heterogeneous Combi-Lipase Biocatalysts Using Wet Extracted Lipids from Microalgae

et al. 2019

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The production of fatty acids ethyl esters (FAEEs) to be used as biodiesel from oleaginous microalgae shows great opportunities as an attractive source for the production of renewable fuels without competing with human food. To ensure the economic viability and environmental sustainability of the microbial biomass as a raw material, the integration of its production and transformation into the biorefinery concept is required. In the present work, lipids from wet Isochrysis galbana microalga were extracted with ethyl acetate with and without drying the microalgal biomass (dry and wet extraction method, respectively). Then, FAEEs were produced by lipase-catalyzed transesterification and esterification of the extracted lipids with ethanol using lipase B from Candida antarctica (CALB) and Pseudomonas cepacia (PC) lipase supported on SBA-15 mesoporous silica functionalized with amino groups. The conversion to FAEEs with CALB (97 and 85.5 mol% for dry and wet extraction, respectively) and PC (91 and 87 mol%) biocatalysts reached higher values than those obtained with commercial Novozym 435 (75 and 69.5 mol%). Due to the heterogeneous nature of the composition of microalgae lipids, mixtures with different CALB:PC biocatalyst ratio were used to improve conversion of wet-extracted lipids. The results showed that a 25:75 combi-lipase produced a significantly higher conversion to FAEEs (97.2 mol%) than those produced by each biocatalyst independently from wet-extracted lipids and similar ones than those obtained by each lipase from the dry extraction method. Therefore, that optimized combi-lipase biocatalyst, along with achieving the highest conversion to FAEEs, would allow improving viability of a biorefinery since biodiesel production could be performed without the energy-intensive step of biomass drying.

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Section: Characterization Of the Synthesized Enzymatic Catalystmentioning

confidence: 99%

Biodiesel Production (FAEEs) by Heterogeneous Combi-Lipase Biocatalysts Using Wet Extracted Lipids from Microalgae

et al. 2019

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“…This observation indicated that the unfolding of RCL was initiated at about 500 MPa and that the salt did not shift the beginning point of unfolding. In addition, the fluorescence intensity at 600 MPa was more than two times of that at atmospheric pressure, indicating that more hydrophobic area exposed to the solvent due to HHP treatment . This result indicated that the presence of KCl cannot prevent and alleviate the high pressure‐induced damage to the interior hydrophobic core of the lipase.…”

Section: Resultsmentioning

confidence: 98%

“…In addition, the fluorescence intensity at 600 MPa was more than two times of that at atmospheric pressure, indicating that more hydrophobic area exposed to the solvent due to HHP treatment. 29,39 This result indicated that the presence of KCl cannot prevent and alleviate the high pressure-induced damage to the interior hydrophobic core of the lipase. It could explain that the addition of KCl cannot improve the RCL unfolding stability at 600 MPa.…”

Section: Denaturation Of Rcl Measured By Ans Fluorescencementioning

confidence: 97%

Coupled effects of salt and pressure on catalytic ability of Rhizopus chinensis lipase

et al. 2017

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“…The MG forms of proteins have remarkable secondary structure and few tertiary contacts related to the native state, but its compactness is somehow less than the native state [35][36][37][38]. However, there is some report that MG has more toxicity effects, but its mechanisms remain unknown.…”

Section: Discussionmentioning

confidence: 96%

Interaction of insulin with methyl tert -butyl ether promotes molten globule-like state and production of reactive oxygen species

Valipour

Maghami

Habibi-Rezaei

et al. 2015

International Journal of Biological Macromolecules

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Impact of structural stability of cold adapted Candida antarctica lipase B (CaLB): in relation to pH, chemical and thermal denaturation

Abstract: The effect of pH on the conformational behavior of Candida antartica lipase B (CaLB) has been monitored by spectroscopic and calorimetric studies.

Cited by 162 publications

References 51 publications

Biodiesel Production (FAEEs) by Heterogeneous Combi-Lipase Biocatalysts Using Wet Extracted Lipids from Microalgae

Biodiesel Production (FAEEs) by Heterogeneous Combi-Lipase Biocatalysts Using Wet Extracted Lipids from Microalgae

Coupled effects of salt and pressure on catalytic ability of Rhizopus chinensis lipase

Interaction of insulin with methyl tert -butyl ether promotes molten globule-like state and production of reactive oxygen species

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