Interaction of insulin with methyl tert -butyl ether promotes molten globule-like state and production of reactive oxygen species

Valipour, Masoumeh; Maghami, Parvaneh; Habibi-Rezaei, Mehran; Sadeghpour, Mostafa; Khademian, Mohamad Ali; Mosavi, Khadijeh; Moosavi-Movahedi, Ali Akbar

doi:10.1016/j.ijbiomac.2015.07.030

Cited by 21 publications

(14 citation statements)

References 47 publications

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“…Insulin is one of the most important blood proteins, which contains 51 amino acid residues in its structure and mediates glucose uptake. Insulin consists of 21-residues in the A chain and 30 residues in the B chain, which are tethered by one intra-chain (A6-A11) and two inter-chains ((A7-B7) and (A20-B19)) disulfide bonds [28,29]. Insulin as a control hormone binds to its receptor and regulates metabolism by suppressing gluconeogenesis enzymes.…”

Section: Introductionmentioning

confidence: 99%

Unfolding of insulin at the surface of ZnO quantum dots

Hosseinzadeh

Saboury

Moosavi-Movahedi

2016

International Journal of Biological Macromolecules

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ZnO quantum dots (QDs) have been used in many biomedical applications such as bioimaging, cancer treatments and etc. Crystallinity, particle size, optical absorption and photoluminescence spectra of ZnO QDs were analyzed by X-ray diffraction (XRD), transmission electron microscopy (TEM), UV-vis absorption spectroscopy and fluorescence spectroscopy respectively. Interaction of ZnO QDs with insulin was investigated by fluorescence quenching, circular dichroism (CD), isothermal titration calorimetry (ITC) and thermal aggregation tests. The fluorescence quenching results showed a static type quenching along with red shift in synchronize fluorescence (a sign of protein unfolding). CD spectroscopy results also confirmed this unfolding and show a reduction in alpha helices content of insulin in contact with ZnO QDs and their conversion to random coils. According to ITC results, the ΔG, ΔH and binding constant of this interaction are -32.35 kJ/mol, -43.21 kJ/mol and 4.69 × 10(5) M(-1), respectively. Thermal aggregation test showed fast aggregation of insulin in the presence of ZnO QDs. Therefore in biological application of ZnO QDs such as bioimaging, presence of such QDs in vicinity of insulin could unfold this protein.

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Section: Introductionmentioning

confidence: 99%

Unfolding of insulin at the surface of ZnO quantum dots

Hosseinzadeh

Saboury

Moosavi-Movahedi

2016

International Journal of Biological Macromolecules

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“…Research on humans has failed to provide conclusive results, although there have been reports of nephrotoxicity, depression of the central nervous system, migraine, tremors, ataxia, cough, and dyspnea in exposed participants. To identify the molecular interaction of MTBE with human proteins, we previously reported that MTBE promotes the molten globule‐like state and structural changes in insulin . These findings confirmed the potential toxicity of MTBE and might be relevant to understanding the effects observed in vivo …”

Section: Discussionmentioning

confidence: 53%

“…A chemiluminescence spectroscopy–based method was used for the determination of the reactive oxygen species (ROS) production capability of MTBE in its interaction with Hb . Experimental details of this test were as follow: 5 μL of HAuCl 4 solution (dissolved in distilled water) and 5 μL Luminol solution (dissolved in 100mM sodium carbonate buffer; pH 11) were injected into the buffer, Hb, or mixture of MTBE and Hb samples, and the chemiluminescence data were immediately recorded at a wavelength of 425 nm using a chemiluminescence spectrophotometer (Synergy H4 Hybrid Reader; BioTek, United States).…”

Section: Methodsmentioning

confidence: 99%

Antichaperone activity and heme degradation effect of methyl tert‐butyl ether (MTBE) on normal and diabetic hemoglobins

Najdegerami

Maghami

Sheikh-Hasani

et al. 2016

J of Molecular Recognition

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Because of the extensive use of methyl tert-butyl ether (MTBE) as an additive to increase the octane quality of gasoline, the environmental pollution by this compound has increased in recent decades. Environmental release of MTBE may lead to its entry to the blood stream through inhalation or drinking of contaminated water, and its interactions with biological molecules such as proteins. The present study was proposed to comparatively investigate the interactions of MTBE with hemoglobin (Hb) from diabetic and nondiabetic individuals using various spectroscopic methods including UV-visible, fluorescence, chemiluminescence, and circular dichroism. These results demonstrated the effects of MTBE on heme degradation of Hb and the reaction of these degradation products with water generating reactive oxygen species. Interaction of Hb with MTBE enhanced its aggregation rate and decreased lag time, indicating the antichaperone activity of MTBE upon interaction with Hb. Furthermore, the diabetic Hb showed more severe effects of MTBE, including heme degradation, reactive oxygen species production, unfolding, and antichaperone behavior than the nondiabetic Hb. The results from molecular docking suggested that the special interaction site of MTBE in the vicinity of Hb heme group is responsible for heme degradation.

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“…For instance, benzene which is a constituent of petrol can cause oxidative stress by undergoing metabolic activation resulting in generation of reactive oxygen species (15). Another important constituent of petrol, methyl tertiary butyl ether (MTBE) is predominantly metabolized in the liver and its toxicity may be ascribed to induction of oxidative changes (20,41,42). A recent study (34) could not establish significant oxidative changes in the erythrocytes of rats exposed to petrol vapours, however, another study (8) demonstrated that exposure to petrol vapours was associated with oxidative stress in liver cells of male and female rats.…”

Section: Discussionmentioning

confidence: 99%

The Effects of Honey Administration on Petrol-Induced Oxidative Stress and Hepatotoxicity in Sprague-Dawley Male Rats

Abubakar¹,

Ang²

2020

ACEMedicine

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Background: Exposure to petrol is common among individuals working in oil refineries, oil fields, filling stations, automobile mechanical workshops. Exposure to petrol is associated with a number of health hazards including hepatotoxicity and oxidative stress. Honey is rich in many antioxidants such as catalase, flavonoids, thiamine, riboflavin, pyridoxine, pantothenic acid, ascorbic acid and nicotinic acid and was recently reported to reduce petrol-induced hepatotoxicity and nephrotoxicity. Materials and Methods: A total of 32 male Sprague-Dawley rats were randomly divided into four groups: control group (exposed to ambient air daily), petrol exposed group

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Interaction of insulin with methyl tert -butyl ether promotes molten globule-like state and production of reactive oxygen species

Cited by 21 publications

References 47 publications

Unfolding of insulin at the surface of ZnO quantum dots

Unfolding of insulin at the surface of ZnO quantum dots

Antichaperone activity and heme degradation effect of methyl tert‐butyl ether (MTBE) on normal and diabetic hemoglobins

The Effects of Honey Administration on Petrol-Induced Oxidative Stress and Hepatotoxicity in Sprague-Dawley Male Rats

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