Impact of ultrafiltration and nanofiltration of an industrial fish protein hydrolysate on its bioactive properties

Abstract

International audienceBACKGROUND: Numerous studies have demonstrated that in vitro controlled enzymatic hydrolysis of fish and shellfish proteins leads to bioactive peptides. Ultrafiltration (UF) and/or nanofiltration (NF) can be used to refine hydrolysates and also to fractionate them in order to obtain a peptide population enriched in selected sizes. This study was designed to highlight the impact of controlled UF and NF on the stability of biological activities of an industrial fish protein hydrolysate (FPH) and to understand whether fractionation could improve its content in bioactive peptides. RESULTS: The starting fish protein hydrolysate exhibited a balanced amino acid composition, a reproducible molecular weight (MW) profile, and a low sodium chloride content, allowing the study of its biological activity. Successive fractionation on UF and NF membranes allowed concentration of peptides of selected sizes, without, however, carrying out sharp separations, some MW classes being found in several fractions. Peptides containing Pro, Hyp, Asp and Glu were concentrated in the UF and NF retentates compared to the unfractionated hydrolysate and UF permeate, respectively. Gastrin/cholecystokinin-like peptides were present in the starting FPH, UF and NF fractions, but fractionation did not increase their concentration. In contrast, quantification of calcitonin gene-related peptide (CGRP)-like peptides demonstrated an increase in CGRP-like activities in the UF permeate, relative to the starting FPH. The starting hydrolysate also showed a potent antioxidant and radical scavenging activity, and a moderate angiotensin-converting enzyme (ACE)-1 inhibitory activity, which were not increased by UF and NF fractionation. CONCLUSION: Fractionation of an FPH using membrane separation, with a molecular weight cut-off adapted to the peptide composition, may provide an effective means to concentrate CGRP-like peptides and peptides enriched in selected amino acids. The peptide size distribution observed after UF and NF fractionation demonstrates that it is misleading to characterize the fractions obtained by membrane filtration according to the MW cut-off of the membrane only, as is currently done in the literature. Copyright © 2010 Society of Chemical Industr

ACE inhibitory activity of pangasius catfish (Pangasius sutchi) skin and bone gelatin hydrolysate

“…Besides exploring diverse types of bioactivities such as ACE inhibitory activity, studies focused on the effect of oral intake in both animal and human models have revealed the excellent absorption and metabolism of isolated peptides from gelatin hydrolysates (Gómez-Guillén et al ). The enrichment of fractions or specific peptides with the aim of ultrafiltration has been widely applied in order to produce hydrolysates with improved nutritional or functional properties within specific molecular weight ranges from high molecular mass residues (Harnedy and Fitzgerald 2012;Picot et al 2010). Alcalase manifested extensive proteolytic activity during the hydrolysis of skin gelatin from Alaska pollack, squid Todarodes pacificus and giant squid, producing hydrolysates with low average molecular weight which exhibited high ACE inhibitory capacity (Giménez et al 2009;Nam et al 2008).…”

Efecto de Temperatura, pH, Concentración de Sustrato y Tipo de Enzima en la Hidrólisis Enzimática de Vísceras de Tilapia Roja (Oreochromis spp.)

“…Los cuales dependiendo de su composición, secuencia y número de aminoácidos (generalmente entre 2 -20), pueden poseer actividades biológicas, como: antihipertensiva, antioxidante, inmunomoduladora, antitrombótica, anticancerígena, entre otras (Lee et al, 2010;Picot et al, 2010;Wang et al, 2013;Deraz, 2014;Shahidi y Ambigaipalan, 2015). Diferentes investigaciones, han establecido la relación existente entre la actividad biológica de los péptidos y su peso molecular (Bourseau et al, 2009;Picot et al, 2010;Saidi et al, 2013;Wang et al, 2013;Liu et al, 2014). En particular, las fracciones de péptidos con pesos moleculares entre 1-4kDa, serían los más interesantes para usos nutricionales y/o farmacéuticos (Saidi et al, 2014;de Castro y Sato, 2015;Opheim et al, 2015).…”

Aktivitas Antibakteri Dan Antioksidan Hidrolisat Hasil Hidrolisis Protein Susu Kambing Dengan Ekstrak Kasar Bromelin

“…Ultrafiltrasi menggunakan membran molecular weight cut off (MWCO) memungkinkan untuk memekatkan peptida dengan berat molekul tertentu dengan menghilangkan konsentrasi garam dan kontaminan (Vandanjon et al, 2007), sehingga aktivitasnya meningkat. Menurut Picot et al (2010), fraksinasi peptida menggunakan membran MWCO merupakan cara yang efektif untuk memekatkan peptida antioksidan, tetapi dalam prosesnya masih memerlukan optimasi. Selektivitas membran yang digunakan bukan hanya memisahkan peptida berdasarkan ukuran tetapi juga berdasarkan gugus aromatik (Picot et al, 2010) dan muatan (Doyen et al, 2011) yang berpengaruh pada bioaktivitasnya.…”

“…Menurut Picot et al (2010), fraksinasi peptida menggunakan membran MWCO merupakan cara yang efektif untuk memekatkan peptida antioksidan, tetapi dalam prosesnya masih memerlukan optimasi. Selektivitas membran yang digunakan bukan hanya memisahkan peptida berdasarkan ukuran tetapi juga berdasarkan gugus aromatik (Picot et al, 2010) dan muatan (Doyen et al, 2011) yang berpengaruh pada bioaktivitasnya. Menurut Udenigwe dan Aluko (2011) (Udenigwe dan Aluko, 2011).…”