Impact of UV-C Irradiation on Bacterial Disinfection in a Drinking Water Purification System

Kim, Hyun‐Joong; Yoon, Heewon; Lee, Mina; Kim, Young-Hoon; Lee, Chang Joo

doi:10.4014/jmb.2211.11027

Cited by 17 publications

(4 citation statements)

References 37 publications

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“…UVC has been used since the 90´s for water disinfection, since within the non-thermal physical techniques, UVC disinfection stands out as a financially viable and readily method for potable water (25). Furthermore, in recent years, globally, technologies involving household water purifier devices employing cartridge filtration and UV disinfection have gained widespread usage (26).…”

Section: Discussionmentioning

confidence: 99%

BEYOND UNIFORMITY: Pyomelanin’s structural complexity impacts on UV shielding inPseudomonasspecies with different lifestyles

Diaz Appella,

Kolender,

Oppezzo

et al. 2024

Preprint

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Melanin, a polymeric pigment synthesized by various organisms, confers advantageous traits, including heightened resistance to stress agents. InPseudomonas, disruption of the tyrosine degradation pathway leads to pyomelanin production. Despite a shared synthetic pathway, the chemical structure of pyomelanin remains elusive due to its heterogeneous polymeric nature, suggesting composition variations even among closely related species. Our objective was to analyze pyomelanin structural features acrossPseudomonasstrains: CRISPR/nCas9-engineeredhmgAmutants of PAO1 and PA14, reference strains of the human opportunist pathogenP. aeruginosa; a natural melanogenic mutant (PAM) from a patient; and a Tn5 mutant of the extremophile bacteriumP. extremaustralis(PexM). Structural analysis revealed strain-specific differences. UV spectra exhibited dual peaks for PAO1 and PA14 mutants, while PAM and PexM displayed a single peak. FTIR indicated changes in the alcohol content ratio, with PAO1 and PA14hmgAmutants having a near 1:1 ratio, PexM a dominant phenol band, and PAM a predominance of the alcohol band. Complex NMR spectra suggested non-linear polymers composed by substituted phenolic rings, carboxylic acids, and alkyl chains, highlighting inter-pigment disparities. UVC (254 nm) susceptibility assessment showed increased survival with pyomelanin addition, correlating with the attenuation of the incoming radiation due to absorption in the culture medium. Moreover, survival to UVC ofP. extremaustralisdifferent depending on the melanin source being the most protective pyomelanin obtained from PAO1. These findings reveal distinct pyomelanin subgroups based on structure among strains, elucidating varied physiological effects.

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Section: Discussionmentioning

confidence: 99%

BEYOND UNIFORMITY: Pyomelanin’s structural complexity impacts on UV shielding inPseudomonasspecies with different lifestyles

Diaz Appella,

Kolender,

Oppezzo

et al. 2024

Preprint

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“…UV irradiation might have limitations in inactivating pathogens in turbid liquid environments. However, it was found elsewhere that UVC irradiation (irradiation dose of 40 mJ/cm 2 ) was able to affect the growth of Shigella flexneri and Listeria monocytogenes in drinking water [35]. The agar is nutrient-rich and, therefore, bacteria have the required medium for growth and conditions at 37 • C. The temperature was not maintained throughout the experiment, which can impact the number of bacteria.…”

Section: Discussionmentioning

confidence: 99%

A Pilot Study to Investigate the Antimicrobial Activity of Pulsed UVA and UVC

Hunter,

Percival,

Eberl

et al. 2023

Aerobiology

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UV irradiation has shown potential in reducing bacterial and viral loadings. This is a pilot study aimed at investigating the antimicrobial effect of a novel pulsed UVA and UVC technology on bacteria and human coronavirus 229E. The selection of these microorganisms is based on their relevance and significance in real-world scenarios. This study consists of independent experiments for the assessment of antibacterial and antiviral activities by using a lawn plate approach, measuring levels of adenine triphosphate (ATP) in three bacterial strains, Escherichia coli, Staphylococcus epidermidis and Bacillus subtilis, and performing Median Tissue Culture Infectious Dose (TCID50) of HCoV-229E on MRC-5 human lung fibroblast cell line. The results demonstrated the ability of UVA and UVC irradiation to reduce levels of adenine triphosphate (ATP) over a 12 h exposure period in all three bacterial strains, comparative to dark and artificial/natural light conditions using non-pulsing experiments. In addition to this, there was a reduction in colonies exposed to UVA and UVC pulsing experiments for E. coli K12 and S. epidermis compared to bacteria stored in artificial/natural and dark conditions. Furthermore, using dose-dependent modelling, it was demonstrated that the cross-contamination risk was reduced by 50% using E. coli as a typical model. Regarding the antiviral assay, the results showed that TCID50 of HCoV-229E was reduced after the first cycle of UV engagement. No cytopathic effect (CPE) was detected after three cycles using Protocol 1. The findings showed that UVA and UVC were effective under the conditions outlined in this paper for a reduction in the number of bacteria with additional applications to viruses.

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“…A previous study showed that the UV-C irradiation of drinking water inhibited the growth of both Gram-negative and Gram-positive bacteria [29]. In addition, a UV-C treatment minimized alterations in the quality of semi-salted mackerel fillets and inactivated S. aureus [30].…”

Section: Inhibitory Effects Of Salting and Uv-c Irradiation On S Aure...mentioning

confidence: 99%

Microbiological Analysis of Manufacturing Processes and Microbial Hazard Assessment of Quality and Safety of Commercial Salted Shrimp (Saeu-jeot)

Jeong,

Lee,

et al. 2024

Fishes

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The objective of this study was to evaluate the microbiological properties of commercially available traditional Korean salted shrimp (Saeu-jeot) and to analyze the effects of saltwater immersion and ultraviolet (UV) irradiation on the reduction of Staphylococcus aureus in Saeu-jeot. A microbiological analysis was conducted across 56 commercially available Saeu-jeot samples from the Korean, Chinese, and Vietnamese markets. The microbiological analysis revealed no presence of Escherichia coli, coliforms, or Vibrio parahaemolyticus in any commercial samples. The total viable count and S. aureus were 3.8 ± 0.4 and 0.6 ± 0.3 log CFU/g, respectively. An investigation of the procedures conducted on Saeu-jeot samples at various production stages by Hazard Analysis Critical Control Point (HACCP)-certified companies demonstrated the substantial relevance of the raw material (tiny shrimp) on S. aureus counts. In order to reduce S. aureus in Saeu-jeot, saltwater immersion and UV irradiation treatments were applied, which reduced the S. aureus counts by 1.4 log CFU/g and 0.3 log CFU/g, respectively, and S. aureus’s efficacy was limited to the food’s surface. These results suggest that a co-treatment of saltwater immersion and UV irradiation could be effective in reducing S. aureus. The maintenance of hygienic handling and cleanliness are essential in the modern manufacturing processes of Saeu-jeot.

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Impact of UV-C Irradiation on Bacterial Disinfection in a Drinking Water Purification System

Cited by 17 publications

References 37 publications

BEYOND UNIFORMITY: Pyomelanin’s structural complexity impacts on UV shielding inPseudomonasspecies with different lifestyles

BEYOND UNIFORMITY: Pyomelanin’s structural complexity impacts on UV shielding inPseudomonasspecies with different lifestyles

A Pilot Study to Investigate the Antimicrobial Activity of Pulsed UVA and UVC

Microbiological Analysis of Manufacturing Processes and Microbial Hazard Assessment of Quality and Safety of Commercial Salted Shrimp (Saeu-jeot)

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