Impact of Zinc- or Copper-Doped Mesoporous Bioactive Glass Nanoparticles on the Osteogenic Differentiation and Matrix Formation of Mesenchymal Stromal Cells

Westhauser, Fabian; Decker, Simon; Nawaz, Qaisar; Rehder, Felix; Wilkesmann, Sebastian; Moghaddam, Arash; Kunisch, Elke; Boccaccını, Aldo R.

doi:10.3390/ma14081864

Cited by 28 publications

(25 citation statements)

References 51 publications

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“…In this study, three different B-doped ICIE16-based BGs, namely 3B-BG, 4B-BG and 8B-BG were evaluated regarding their biocompatibility, as well as their influence on osteogenic differentiation and angiogenesis. An indirect cultivation setting was used to assess the impact of B-doped BGs on cells that are not directly adjacent to the material, but only exposed to the BGs’ IDPs 36 , 37 . The study was divided into an in vitro and an in ovo part.…”

Section: Discussionmentioning

confidence: 99%

“…The study was divided in an in vitro and an in ovo part. In the in vitro part, the influence of IDPs of the BGs on BMSCs was assessed using an indirect cultivation setting as published previously 36 , 37 . The different BGs were added to cell culture medium (CCM; DMEM high glucose, 10% FCS, 100 µg/ml penicillin/streptomycin) at a concentration of 1 mg/ml and incubated under standard cell culture conditions.…”

Section: Methodsmentioning

confidence: 99%

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In vitro and in ovo impact of the ionic dissolution products of boron-doped bioactive silicate glasses on cell viability, osteogenesis and angiogenesis

Decker

Arango‐Ospina

Rehder

et al. 2022

Sci Rep

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Due to the pivotal role of angiogenesis in bone regeneration, the angiogenic properties of biomaterials are of high importance since they directly correlate with the biomaterials’ osteogenic potential via ‘angiogenic-osteogenic coupling’ mechanisms. The impact of bioactive glasses (BGs) on vascularization can be tailored by incorporation of biologically active ions such as boron (B). Based on the ICIE16-BG composition (in mol%: 49.5 SiO2, 36.3 CaO, 6.6 Na2O, 1.1 P2O5, 6.6 K2O), three B-doped BGs have been developed (compositions in mol%: 46.5/45.5/41.5 SiO2, 36.3 CaO, 6.6 Na2O, 1.1 P2O5, 6.6 K2O, 3/4/8 B2O3). The influence of B-doping on the viability, cellular osteogenic differentiation and expression of osteogenic and angiogenic marker genes of bone marrow-derived mesenchymal stromal cells (BMSCs) was analyzed by cultivating BMSCs in presence of the BGs’ ionic dissolution products (IDPs). Furthermore, the influence of the IDPs on angiogenesis was evaluated in ovo using a chorioallantoic membrane (CAM) assay. The influence of B-doped BGs on BMSC viability was dose-dependent, with higher B concentrations showing limited negative effects. B-doping led to a slight stimulation of osteogenesis and angiogenesis in vitro. In contrast to that, B-doping significantly enhanced vascularization in ovo, especially in higher concentrations. Differences between the results of the in vitro and in ovo part of this study might be explained via the different importance of vascularization in both settings. The implementation of new experimental models that cover the ‘angiogenic-osteogenic coupling’ mechanisms is highly relevant, for instance via extending the application of the CAM assay from solely angiogenic to angiogenic and osteogenic purposes.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

In vitro and in ovo impact of the ionic dissolution products of boron-doped bioactive silicate glasses on cell viability, osteogenesis and angiogenesis

Decker

Arango‐Ospina

Rehder

et al. 2022

Sci Rep

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“…Another promising approach for the treatment of large bone defects could be the combination of S53P4 with autologous/allogenic bone grafts and local or systemic antibiotics. Newly designed bioactive glasses containing high concentrations of lithium ions [ 54 ] or copper-doped glasses [ 55 ] could also be interesting materials to study in this context. Their altered properties compared to S53P4 could lead to more osteoconductive or osteoinductive potential.…”

Section: Discussionmentioning

confidence: 99%

Treatment of Infection-Related Non-Unions with Bioactive Glass—A Promising Approach or Just Another Method of Dead Space Management?

et al. 2022

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The treatment of infected and non-infected non-unions remains a major challenge in trauma surgery. Due to the limited availability of autologous bone grafts and the need for local anti-infective treatment, bone substitutes have been the focus of tissue engineering for years. In this context, bioactive glasses are promising, especially regarding their anti-infective potential, which could reduce the need for local and systemic treatment with conventional antibiotics. The aim of this study was to investigate the osteoinductive and osteoconductive effects, as well as the anti-infectious potential, of S53P4 using a standardized non-union model, which had not been investigated previously. Using an already established sequential animal model in infected and non-infected rat femora, we were able to investigate bioactive glass S53P4 under realistic non-union conditions regarding its osteoinductive, osteoconductive and anti-infective potential with the use of µCT scans, biomechanical testing and histological, as well as microbiological, analysis. Although S53P4 did not lead to a stable union in the non-infected or the infected setting, µCT analysis revealed an osteoinductive effect of S53P4 under non-infected conditions, which was diminished under infected conditions. The osteoconductive effect of S53P4 remained almost negligible in histological analysis, even 8 weeks after treatment. Additionally, the expected anti-infective effect could not be demonstrated. Our data suggested that S53P4 should not be used in infected non-unions, especially in those with large bone defects.

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“…The co-doping of the Zn and Mn metallic ions provides synergetic effect. Zn is proven to be an excellent antibacterial agent thus it will hinder the formation of biofilm on the implant [39,60]. Furthermore, Mn is an excellent osteogenic material widely used in bone tissue engineering [24,61].…”

Section: Epd Of Zein/zn-mn Mbgns (Doe Approach)mentioning

confidence: 99%

“…Zn and Mn are added for antibacterial and osteogenic effect, respectively. The doping of metallic ions in MBGNs to produce synergetic antimicrobial effect and osteogenesis is the recent trend [38,39].…”

Section: Introductionmentioning

confidence: 99%

Zn-Mn Doped Mesoporous Bioactive Glass Nanoparticles Loaded Zein Coatings for Bioactive and Antibacterial Orthopedic Implants

Batool¹,

Kazemi²,

Irfan³

et al. 2022

Preprint

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In recent years, natural polymers have replaced synthetic polymers for antibacterial orthopedic applications owing to their excellent biocompatibility and biodegradability. Zein is a biopolymer found in corn. The lacking mechanical stability of zein is overcome by incorporating bioceramics e.g. mesoporous bioactive glass nanoparticles (MBGNs). In present study, pure zein and zein/Zn-Mn MBGNs composite coatings were deposited via electrophoretic deposition (EPD) on 316L stainless steel (SS). Zn and Mn were co-doped in MBGNs in order to make use of their antibacterial and osteogenic potential, respectively. A Taguchi design of experiment (DoE) study was established to evaluate the effect of various working parameters on the morphology of the coatings. It was observed that coatings deposited at 20 V for 5 min with 4 g/L concentration (conc.) of Zn-Mn MBGNs showed highest deposition yield. Uniform coatings with highly dispersed MBGNs were obtained adopting these optimized parameters. Scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), X-Ray diffraction (XRD), and fourier transform infrared spectroscopy (FTIR) were employed to investigate the morphology and elemental composition of zein/Zn-Mn MBGNs composite coating. Surface properties i.e. coating roughness and wettability analysis concluded that composite coatings were appropriate for cell attachment and proliferation. For adhesion strength various techniques including tape test, bend test, pencil hardness test, and tensile test were performed. Wear and corrosion analysis highlighted the mechanical and chemical stability of coatings. Colony forming unit (CFU) test showed that zein/Zn-Mn MBGNs composite coating was highly effective against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) due to the presence of Zn. The formation of hydroxyapatite (HA) like structure upon immersion in simulated body fluid (SBF) validated the in vitro bioactivity of the coating. It was concluded that zein/Zn-Mn MBGNs coating synthesized in this work can be used for bioactive and antibacterial orthopedic applications.

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Impact of Zinc- or Copper-Doped Mesoporous Bioactive Glass Nanoparticles on the Osteogenic Differentiation and Matrix Formation of Mesenchymal Stromal Cells

Cited by 28 publications

References 51 publications

In vitro and in ovo impact of the ionic dissolution products of boron-doped bioactive silicate glasses on cell viability, osteogenesis and angiogenesis

In vitro and in ovo impact of the ionic dissolution products of boron-doped bioactive silicate glasses on cell viability, osteogenesis and angiogenesis

Treatment of Infection-Related Non-Unions with Bioactive Glass—A Promising Approach or Just Another Method of Dead Space Management?

Zn-Mn Doped Mesoporous Bioactive Glass Nanoparticles Loaded Zein Coatings for Bioactive and Antibacterial Orthopedic Implants

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