Impacts of Citric Acid and Malic Acid on Fermentation Quality and Bacterial Community of Cassava Foliage Silage

Li, Mao; Zhang, Youmin; Zhang, Qing; Zi, Xuejuan; Lv, Renlong; Tang, Jun; Zhou, Hanlin

doi:10.3389/fmicb.2020.595622

Cited by 32 publications

(27 citation statements)

References 33 publications

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“…Bacillus , a type of facultatively anaerobic and Gram-positive bacteria, can secrete bacteriocin and inhibit the activities of undesirable bacteria. In addition, some species of Bacillus can produce LA ( Li M. et al, 2020 ). Weissella , a type of heterofermentative LAB, converts 1 mol glucose to 1 mol AA, 1 mol LA, and 1 mol CO 2 ( Wang M. et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

“…Malic acid has antioxidant and antibacterial functions and is widely used in food, pharmaceutical, healthcare, cosmetics, and other industries. Moreover, malic acid, which can be used as a carbohydrate source, provides energy for LAB and accelerates LAB growth ( Li M. et al, 2020 ). Notably, the relative abundance of differential pathways caused by glucose was lower compared with those of pathways caused by malic acid, cellulase, and B. subtilis .…”

Section: Discussionmentioning

confidence: 99%

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Additives Altered Bacterial Communities and Metabolic Profiles in Silage Hybrid Pennisetum

et al. 2022

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This study was conducted to investigate the effects of different additives on the fermentation quality, nutrient composition, bacterial communities, and metabolic profiles of the silage of hybrid Pennisetum. The experiment was conducted using five treatments, i.e., CK, control group, MA, 1% malic acid of fresh matter (FM) basis, GL, 1% glucose of FM basis, CE, 100 U/g FM cellulase, and BS, 106 cfu/g FM Bacillus subtilis, with six replicates each treatment. After a 120-day fermentation, 30 silage packages were opened for subsequent determination. As a result, all four additives had positive effects on the fermentation quality and nutrient composition of the silage of hybrid Pennisetum. The high-throughput sequencing of V3–V4 regions in 16S rRNA was performed, and results showed that Firmicutes and Proteobacteria were the dominant phyla and that Aquabacterium and Bacillus were the dominant genera. MA, GL, CE, and BS treatment resulted in 129, 21, 25, and 40 differential bacteria, respectively. The four additives upregulated Bacillus smithii but downregulated Lactobacillus rossiae. Metabolic profiles were determined by UHPLC-Q/TOF-MS technology and the differential metabolites caused by the four additives were 47, 13, 47, and 18, respectively. These metabolites played antioxidant, antibacterial, and anti-inflammatory functions and involved in pathways, such as the citrate cycle, carbon fixation in photosynthetic organisms, and glyoxylate and dicarboxylate metabolism. In conclusion, silage additives promoted fermentation quality and nutrient composition by altering bacterial communities and metabolic profiles. This study provided potential biomarkers for the improvement of silage quality.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Additives Altered Bacterial Communities and Metabolic Profiles in Silage Hybrid Pennisetum

et al. 2022

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“…Within silage, Paenibacillus species (spore-forming bacteria) could enhance growth of LAB, tolerate acidic conditions and produce bacteriocins that inhibit yeast and molds ( Xu et al, 2018 ; Singh et al, 2019 ). However, it is important to note that in some cases Paenibacillus can compete with LAB for fermentable sugars, limiting its growth ( Li et al, 2020 ). In addition, they have a vast lignocellulolytic potential that may improve the digestion of complex carbohydrates in the bovine rumen ( Ticona et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

“…However, the presence of FAE was not detected within the reconstructed MAGs. Interestingly, Cellulosimicrobium and Paenibacillus species have been cataloged as desirable microorganisms in cassava foliage silage ( Li et al, 2020 ). Finally, this study reported the design and characterization of a mixed consortium, obtained from the M. maximus phyllosphere, composed of LAB and LB.…”

Section: Discussionmentioning

confidence: 99%

Top-Down Enrichment Strategy to Co-cultivate Lactic Acid and Lignocellulolytic Bacteria From the Megathyrsus maximus Phyllosphere

et al. 2021

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Traditionally, starting inoculants have been applied to improve ensiling of forage used for livestock feed. Here, we aimed to build up a bioinoculant composed of lactic acid-producing and lignocellulolytic bacteria (LB) derived from the Megathyrsus maximus (guinea grass) phyllosphere. For this, the dilution-to-stimulation approach was used, including a sequential modification of the starting culture medium [Man, Rogosa, and Sharpe (MRS) broth] by addition of plant biomass (PB) and elimination of labile carbon sources. Along 10 growth-dilution steps (T1–T10), slight differences were observed in terms of bacterial diversity and composition. After the sixth subculture, the consortium started to degrade PB, decreasing its growth rate. The co-existence of Enterobacteriales (fast growers and highly abundance), Actinomycetales, Bacillales, and Lactobacillales species was observed at the end of the selection process. However, a significant structural change was noticed when the mixed consortium was cultivated in higher volume (500ml) for 8days, mainly increasing the proportion of Paenibacillaceae populations. Interestingly, Actinomycetales, Bacillales, and Lactobacillales respond positively to a pH decrease (4–5), suggesting a relevant role within a further silage process. Moreover, gene-centric metagenomic analysis showed an increase of (hemi)cellulose-degrading enzymes (HDEs) during the enrichment strategy. Reconstruction of metagenome-assembled genomes (MAGs) revealed that Paenibacillus, Cellulosimicrobium, and Sphingomonas appear as key (hemi)cellulolytic members (harboring endo-glucanases/xylanases, arabinofuranosidases, and esterases), whereas Enterococcus and Cellulosimicrobium have the potential to degrade oligosaccharides, metabolize xylose and might produce lactic acid through the phosphoketolase (PK) pathway. Based on this evidence, we conclude that our innovative top-down strategy enriched a unique bacterial consortium that could be useful in biotechnological applications, including the development/design of a synthetic bioinoculant to improve silage processes.

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“…The microbial count of each sample was determined by the method of Cai et al (1999) , and described by Li et al (2020) . In brief, each sample of 10 g was mixed with 90 ml sterile saline, shaken for 30 min and then filtered through sterile gauze.…”

Section: Methodsmentioning

confidence: 99%

Effects of Delayed Harvest and Additives on Fermentation Quality and Bacterial Community of Corn Stalk Silage

Guo

et al. 2021

Front. Microbiol.

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This study aimed to investigate the effects of delayed harvest and additives on the fermentation quality and bacterial community of corn stalk silage in South China. The corn stalks after ear harvest at the 0 day (D0), 7 days (D7), and 15 days (D15) were used to produce small-bale silages. The silages at each harvest time were treated without (control, CK) or with Lactobacillus plantarum (LP) and sodium benzoate (BF). The results showed that delayed harvest increased pH and acetic acid content and reduced lactic acid content in corn stalk silage (p < 0.05). Compared with CK, the additives decreased the contents of butyric acid and ammonia nitrogen (NH3-N; p < 0.05). The silage treated with LP increased the content of lactic acid and decreased pH (p < 0.05); the silage treated with BF decreased counts of coliform bacteria and yeasts and increased residual water soluble carbohydrates (WSC) content (p < 0.05). Single Molecule, Real-Time sequencing (SMRT) revealed that the abundance of L. plantarum increased, while the abundance of Lactobacillus brevis and Lactobacillus ginsenosidimutans decreased with the delayed harvest. Additives influenced the bacterial community structure of corn stalk silage, revealed by enhanced bacterial diversity on D0 and reduced on D7 (p < 0.05). Our research indicated that delayed harvest could exert a positive effect on acetic acid production, and additives could inhibit the butyric acid fermentation and protein degradation of corn stalk silage by shifting bacterial community composition.

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Impacts of Citric Acid and Malic Acid on Fermentation Quality and Bacterial Community of Cassava Foliage Silage

Cited by 32 publications

References 33 publications

Additives Altered Bacterial Communities and Metabolic Profiles in Silage Hybrid Pennisetum

Additives Altered Bacterial Communities and Metabolic Profiles in Silage Hybrid Pennisetum

Top-Down Enrichment Strategy to Co-cultivate Lactic Acid and Lignocellulolytic Bacteria From the Megathyrsus maximus Phyllosphere

Effects of Delayed Harvest and Additives on Fermentation Quality and Bacterial Community of Corn Stalk Silage

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