BACKGROUND: Slow-release fertilizer is widely used in cereal crop production because it is ecofriendly and laborsaving. Effects of different application stages (zero-, three-, and six-leaf stages, denoted as SN0, SN3, and SN6, respectively) of slow-release (N/P 2 O 5 /K 2 O = 225/75/75 kg ha −1 ) fertilizer on physicochemical properties of starch from spring-sown waxy maize were investigated in 2018 and 2019. Application of traditional fertilizer (NCK, compound fertilizer; N/P 2 O 5 /K 2 O = 75/75/75 kg ha −1 ) at sowing time and urea (N = 150 kg ha −1 ) at six-leaf stage was designated as the control.RESULTS: In comparison to the NCK, SN0 reduced grain starch content by 4.9%. Meanwhile, SN3 and SN6 did not affect this parameter. Nevertheless, all treatments, particularly SN6, increased average starch granule size. The slow-release fertilizer reduced proportion of chains with degree of polymerization (DP) > 24. Relative to NCK, SN6 increased starch crystallinity in both years, whereas SN0 and SN3 increased it in 2018 but reduced it in 2019. SN0 reduced peak, trough, and final viscosities, whereas SN3 and SN6 produced similar starch viscosities to those produced by NCK. No fertilizer mode affected gelatinization parameters, but SN6 produced a low retrogradation percentage. In comparison to data for 2018, starch produced in 2019 showed a small granule size, and a high proportion of short amylopectin chains. These properties endowed starch with high viscosity and low retrogradation percentage.CONCLUSION: In spring-sown waxy maize production, applying slow-release fertilizer at the six-leaf stage produced starch with high viscosity and low retrogradation tendency by enlarging granule size, increasing crystallinity, and reducing the proportion of long chains.