Although the occurrence, fate and behaviour of micropollutants in the urban water cycle is reported extensively in literature, the biotransformation of micropollutants in the sewer network has only received little attention. Rising main (RM) and gravity main sewer biofilms (GS) are capable of transforming sulfur species and organic compounds biochemically. Moreover, first laboratory-based and full-scale studies demonstrated the capability of sewer biofilms to transform illicit drugs, human drug metabolites and other human biomarkers, such as caffeine. However, the in-sewer biotransformation of pharmaceutically active compounds