Impaired bone healing pattern in mice with ovariectomy-induced osteoporosis: A drill-hole defect model

He, Yang; Zhang, Ge; Pan, Xiaohua; Liu, Zhong; Zheng, Lizhen; Chan, C. W.; Lee, Kwong-Man; Cao, Yongping; Li, Gang; Wei, Lei; Hung, L.K.; Leung, Kwok‐Sui; Qin, Ling

doi:10.1016/j.bone.2011.03.720

Cited by 172 publications

(155 citation statements)

References 51 publications

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“…reported previously by Yi-Xin He et al, (2011), who had studied the bone healing pattern in mice by inducing ovariectomy-induced osteoporosis [21]. This fact was also confirmed by Aerssens et al, (1993), who observed the affect of oestrogen and progesterone indirectly by performing ovariectomies in female rats [22].…”

Section: Resultssupporting

confidence: 63%

Influence of Anabolic Steroid on Tibial Fracture Healing in Rabbits – A Study on Experimental Model

Ahmad¹

2013

JCDR

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Introduction: Anabolic steroid is an established drug for treating catabolic states. The said drug has been shown to restore the lean body mass, to prevent osteoporosis and to correct the impaired immune response, but only few studies have evaluated the effect of the same on fracture healing. Methods:Fracture was produced by an impact device in 24 rabbits which were divided into experimental (I) and control (II) groups of 12 animals each. The experimental groups were further divided into the subgroups IA and IB of equal animals, which received Nandrolone Decanoate 10 mg/kg intramuscularly, biweekly for 2 weeks and 4 weeks respectively.

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Section: Resultssupporting

confidence: 63%

Influence of Anabolic Steroid on Tibial Fracture Healing in Rabbits – A Study on Experimental Model

Ahmad¹

2013

JCDR

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“…In the rodent drill-hole defect model, however, intramembranous ossification is likely dominant. 17,44 Thus, the feasibility of the present technique in conjunction with histological procedures should be confirmed in bone fractures with variable patterns of distraction and mechanical instability that will generate complexly intertwined vascular and bone structures compared with those presently observed.…”

Section: Discussionmentioning

confidence: 85%

“…High-resolution X-ray micro-computed tomography (mCT) combined with a vascular casting technique has been used for three-dimensional (3D) imaging of bone microvasculature in rodents. [14][15][16][17][18] In this technique, barium sulfate-gelatin or lead chromate-loaded silicon has been used as a vascular contrast-casting agent, and bone was often decalcified for extracting microvessels as the reconstructed absorption values in microvessels and bone do not differ much. Decalcification is disadvantageous for investigating the involvement of angiogenesis in bone repair.…”

mentioning

confidence: 99%

Subtraction micro-computed tomography of angiogenesis and osteogenesis during bone repair using synchrotron radiation with a novel contrast agent

Matsumoto

Goto

Sato

2013

Laboratory Investigation

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Quantitative three-dimensional (3D) imaging of angiogenesis during bone repair remains an experimental challenge. We developed a novel contrast agent containing 0.07-to 0.1-mm particles of zirconium dioxide (ZrCA) and established subtraction mCT using synchrotron radiation (sSRCT) for quantitative imaging of angiogenesis and bone repair. This method was applied to a rat model of tibial bone repair 3 days (DAY3; n ¼ 2), 5 days (DAY5; n ¼ 8), or 10 days (DAY10; n ¼ 8) after drill-hole injury. Using the same drill-hole defect model, its potential use was illustrated by comparison of bone repair between hindlimbs subjected to mechanical unloading (n ¼ 6) and normal weight bearing (n ¼ 6) for 10 days. Following vascular casting with ZrCA, the defect site was scanned with 17.9-and 18.1-keV X-rays. In the latter, image contrast between ZrCA-filled vasculature and bone was enhanced owing to the sharp absorption jump of zirconium dioxide at 18.0 keV (k-edge). The two scan data sets were reconstructed with 2.74-mm voxel resolution, registered by mutual information, and digitally subtracted to extract the contrast-enhanced vascular image. K 2 HPO 4 phantom solutions were scanned at 17.9 keV for quantitative evaluation of bone mineral. Angiogenesis had already started, but new bone formation was not found on DAY3. New bone emerged near the defect boundary on DAY5 and took the form of trabecular-like structure invaded by microvessels on DAY10. Vascular and bone volume fractions, blood vessel and bone thicknesses, and mineralization were higher on DAY10 than on DAY5. All these parameters were found to be decreased after 10 days of hindlimb unloading, indicating the possible involvement of angiogenesis in bone repair impairment caused by reduced mechanical stimuli. In conclusion, the combined technique of sSRCT and ZrCA vascular casting is suitable for quantitative 3D imaging of angiogenesis and its surrounding bone regeneration. This method will be useful for better understanding the linkage between angiogenesis and bone repair.

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“…In this study, we selected the distal femur, proximal tibia, and lumbar vertebrae for the representative areas because most of the articles analyzed these areas for their micro-CT analysis among the various trabecular bone-rich regions. 19,[26][27][28][29][30][31][32] Moreover, anatomically these areas are relatively larger in marrow cavity and hence have a greater quantity of trabecular bone; this makes quantification easier and more reproducible. For example, in the femur and tibia, distal femur is bigger than the proximal femur and proximal tibia is bigger than the distal tibia.…”

Section: Discussionmentioning

confidence: 99%

Guidelines for Dual Energy X-Ray Absorptiometry Analysis of Trabecular Bone-Rich Regions in Mice: Improved Precision, Accuracy, and Sensitivity for Assessing Longitudinal Bone Changes

Shi

Lee

Uyeda

et al. 2016

Tissue Engineering Part C: Methods

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Trabecular bone is frequently studied in osteoporosis research because changes in trabecular bone are the most common cause of osteoporotic fractures. Dual energy X-ray absorptiometry (DXA) analysis specific to trabecular bone-rich regions is crucial to longitudinal osteoporosis research. The purpose of this study is to define a novel method for accurately analyzing trabecular bone-rich regions in mice via DXA. This method will be utilized to analyze scans obtained from the International Space Station in an upcoming study of microgravity-induced bone loss. Thirty 12-week-old BALB/c mice were studied. The novel method was developed by preanalyzing trabecular bone-rich sites in the distal femur, proximal tibia, and lumbar vertebrae via high-resolution X-ray imaging followed by DXA and micro-computed tomography (micro-CT) analyses. The key DXA steps described by the novel method were (1) proper mouse positioning, (2) region of interest (ROI) sizing, and (3) ROI positioning. The precision of the new method was assessed by reliability tests and a 14-week longitudinal study. The bone mineral content (BMC) data from DXA was then compared to the BMC data from micro-CT to assess accuracy. Bone mineral density (BMD) intra-class correlation coefficients of the new method ranging from 0.743 to 0.945 and Levene's test showing that there was significantly lower variances of data generated by new method both verified its consistency. By new method, a Bland-Altman plot displayed good agreement between DXA BMC and micro-CT BMC for all sites and they were strongly correlated at the distal femur and proximal tibia (r = 0.846, p < 0.01; r = 0.879, p < 0.01, respectively). The results suggest that the novel method for site-specific analysis of trabecular bone-rich regions in mice via DXA yields more precise, accurate, and repeatable BMD measurements than the conventional method.

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Impaired bone healing pattern in mice with ovariectomy-induced osteoporosis: A drill-hole defect model

Cited by 172 publications

References 51 publications

Influence of Anabolic Steroid on Tibial Fracture Healing in Rabbits – A Study on Experimental Model

Influence of Anabolic Steroid on Tibial Fracture Healing in Rabbits – A Study on Experimental Model

Subtraction micro-computed tomography of angiogenesis and osteogenesis during bone repair using synchrotron radiation with a novel contrast agent

Guidelines for Dual Energy X-Ray Absorptiometry Analysis of Trabecular Bone-Rich Regions in Mice: Improved Precision, Accuracy, and Sensitivity for Assessing Longitudinal Bone Changes

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