Impaired Eyeblink Conditioning and Decreased Hippocampal Volume in PDAPP V717F Mice

Weiss, Craig; Venkatasubramanian, Palamadai N.; Aguado, Allison; Power, John M.; Tom, Brian C.; Li, L.; Chen, K.S.; Disterhoft, John F.; Wyrwicz, Alice M.

doi:10.1006/nbdi.2002.0555

Cited by 64 publications

(44 citation statements)

References 38 publications

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“…This is an excellent system to examine interactions of stress and learning, but the mouse would be a better subject for the purpose of examining the genetic basis of these interactions. Several laboratories have already demonstrated EBC in the mouse (Chen et al 1999;Takehara et al 2002;Weiss et al 2002), including hippocampally dependent trace EBC (Takehara et al 2002;Tseng et al 2004). The aims of the present set of experiments were to determine if stressors that facilitate EBC in the rat also facilitate EBC in the mouse, and to determine if the facilitation is due in part to an increased excitability of CA1 hippocampal pyramidal neurons.…”

mentioning

confidence: 95%

Acute stress facilitates trace eyeblink conditioning in C57BL/6 male mice and increases the excitability of their CA1 pyramidal neurons

Weiss¹,

Sametsky²,

Sasse³

et al. 2005

Learn. Mem.

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The effects of stress (restraint plus tail shock) on hippocampus-dependent trace eyeblink conditioning and hippocampal excitability were examined in C57BL/6 male mice. The results indicate that the stressor significantly increased the concentration of circulating corticosterone, the amount and rate of learning relative to nonstressed conditioned mice, and the excitability of CA1 hippocampal pyramidal neurons. Behaviorally, there was no effect of the stressor on control mice that received unpaired presentations of the tone and periorbital shock, i.e., neither stressed nor nonstressed control mice showed an increase in conditioned responding that was above baseline levels. Biophysically, the stressor significantly decreased the amplitude of the post-burst afterhyperpolarization (AHP) and decreased spike frequency accommodation relative to cells from nonstressed control mice. The effect was significant for mice that were stressed either 1 h or 24 h earlier. The results suggest that the stressor increases the excitability of hippocampal pyramidal neurons and that the mechanism underlying this increase may contribute to the more rapid acquisition of hippocampally dependent eyeblink conditioning.The hippocampus is critically important for the formation of memories, as observed in human patients with temporal lobe damage (Scoville and Milner 1957;Daum et al. 1993;Zola and Squire 2001) and as demonstrated by lesion studies in several species, including rabbits (Solomon et al. 1986;Moyer Jr. et al. 1990), rats (Weiss et al. 1999a) and mice (Takehara et al. 2002;Tseng et al. 2004). Electrophysiological studies using both extracellular single neuron recordings (Berger et al. 1983;Weiss et al. 1996;McEchron and Disterhoft 1997) and intracellular recordings of the biophysical properties of hippocampal pyramidal neurons (Disterhoft et al. 1986;Moyer Jr. et al. 1996) have also revealed learning and memory-specific changes in the firing patterns and biophysical properties of these neurons. These neurons are particularly responsive to behaviorally salient stimuli, especially those that help inform the animal of where it is in space (Wilson and McNaughton 1993) and those that predict the temporal occurrence of another stimulus (Berger et al. 1976).This innate responsiveness is also sensitive to adverse environmental conditions or stressors, which can either facilitate or inhibit the formation of new memories (Kim and Yoon 1998;de Kloet et al. 1999). The relationship between facilitation and inhibition for a particular stressor is likely due to the stimulus intensity in terms of magnitude, frequency, or duration (Shors and Servatius 1997), as well its timing relative to another event (Shors 2001). This relationship is often referred to as having an inverted U function; i.e., some stress facilitates behavior, but too much stress is detrimental. A better understanding of the relation which governs the effects of stress and behavior will be beneficial for understanding the neurobiological basis for stress-related disorders (Brewin 20...

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confidence: 95%

Acute stress facilitates trace eyeblink conditioning in C57BL/6 male mice and increases the excitability of their CA1 pyramidal neurons

Weiss¹,

Sametsky²,

Sasse³

et al. 2005

Learn. Mem.

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“…At fields of at least 7 Tesla (T), and without using contrast agent, anatomical studies on experimental models have generally been carried out using T2-weighted sequences (Spittaels et al, 2002;Wolf et al, 2002;Weiss et al, 2002). Data can be acquired in any direction as an entire three-dimensional set (3D-MRI), or as a set of consecutive slices (2D-MRI).…”

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confidence: 99%

Increased Dosage of DYRK1A and Brain Volumetric Alterations in a YAC Model of Partial Trisomy 21

Sebrié

Chabert

Ledru

et al. 2008

The Anatomical Record

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A yeast artificial chromosome (YAC) transgenic murine model of partial trisomy 21 overexpressing five human genes-including DYRK1A, which encodes a serine threonine kinase involved in cell cycle controlhas been shown to present an increase in brain weight. We analyzed this new phenotype by measuring total and regional brain volumes at different ages, using a 7 Tesla magnetic resonance imaging volumetric approach. Volumetric measurements showed a total volume increase of 13.6% in adult mice. Changes in brain morphogenesis were already visible at a very early postnatal stage (postnatal days 2-7). Region-specific changes were characterized from postnatal day 15 to 5 months. These results, made it possible to define region-specific effects of DYRK1A overexpression, with the strongest increase seen in the thalamus-hypothalamus area (24%).

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“…Note that the entire unconditioned response was recorded. The raw EMG signal can be analyzed by thresholding the signal and counting spikes that cross the level (Weiss et al, 2002) or by integrating the activity and analyzing it as an analog signal . Figure 7 shows a different example of a response that was recorded simultaneously with both the infrared reflective sensor (7A) and with EMG recording wires (7B) from a conditioned mouse.…”

Section: Blink Detectormentioning

confidence: 99%

“…Most laboratories using rodents to study eyeblink conditioniong use a tether to allow limited movement within an arena and to connect signals from the subject to the equipment (Bangasser & Shors, 2007;Stanton, Peloso, Brown & Rodier, 2007;Freeman et al, 2007;TakeharaNishiuchi, 2006;Weiss et al, 2002). A periorbital shock is one such signal that is often used as an unconditioned stimulus (US) to evoke a blink.…”

Section: Introductionmentioning

confidence: 99%

Evoking blinks with natural stimulation and detecting them with a noninvasive optical device: A simple, inexpensive method for use with freely moving animals

Weiss

Disterhoft

2008

Journal of Neuroscience Methods

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Many laboratories studying eyeblinks in unanesthetized rodents use a periorbital shock to evoke the blink. The stimulus is typically delivered via a tether and usually obliterates detection of a full unconditioned response with electromyographic (EMG) recording. Here we describe the adapter we have used successfully for several years to deliver puffs of air to the cornea of freely moving rats during our studies of eyeblink conditioning. The stimulus evokes an unconditioned response that can be recorded without affecting the EMG signal. This allows a complete analysis of the unconditioned response which is important for studies examining reflex modification or the effect of drugs, genetic manipulations, or aging on the unconditioned blink reflex. We also describe an infrared reflective sensor that can be added to the tether to minimize the number of wires that need to be implanted around the eye, and which is relatively immune to electrical artifacts associated with a periorbital shock stimulus or other devices powered by alternating current. The responses recorded simultaneously by EMG wires and the optical sensor appear highly correlated and demonstrate that the optical sensor can measure responses that might otherwise be lost due to electrical interference from a shock stimulus.

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Impaired Eyeblink Conditioning and Decreased Hippocampal Volume in PDAPP V717F Mice

Cited by 64 publications

References 38 publications

Acute stress facilitates trace eyeblink conditioning in C57BL/6 male mice and increases the excitability of their CA1 pyramidal neurons

Acute stress facilitates trace eyeblink conditioning in C57BL/6 male mice and increases the excitability of their CA1 pyramidal neurons

Increased Dosage of DYRK1A and Brain Volumetric Alterations in a YAC Model of Partial Trisomy 21

Evoking blinks with natural stimulation and detecting them with a noninvasive optical device: A simple, inexpensive method for use with freely moving animals

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