Impaired induction of vasoactive intestinal polypeptide after sciatic nerve injury in the streptozotocin-diabetic rat

Calcutt, Nigel A.; Mizisin, Andrew P.; Yaksh, Tony L.

doi:10.1016/0022-510x(93)90128-l

Cited by 13 publications

(4 citation statements)

References 65 publications

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“…After nerve transection, VIP synthesis increases in dorsal root ganglion neurons and levels rise quickly in nerve segments proximal to the lesion where regeneration begins (Nielsch and Kean, 1989;Doughty et al, 1991;Rayan et al, 1991;Kashiba et al, 1992;Calcutt et al, 1993;Noguchi et al, 1993). In a previous study, we showed that supernatants prepared from proximal segments of nerves 24-48 hr after transection increased laminin production in Schwann cells cultured in the absence of neurons (Zhang et al, 1994).…”

Section: Discussionmentioning

confidence: 98%

“…In the PNS, VIP is synthesized in autonomic and dorsal root ganglion neurons, transported in their axons and peripheral processes, and has been identified in their synapses and endings (Giachetti and Said, 1979;Lundberg et al, 1981;Hokfelt et al, 1982;Fahrenkrug, 1982;Rayan et al, 1991;Calcutt et al, 1993). After nerve transection, VIP expression is upregulated in sensory neurons and in nerve segments proximal to the lesion where axon regeneration begins (Nielsch and Keen, 1989;Doughty et al, 1991;Rayan et al, 1991;Kashiba et al, 1992;Calcutt et al , 1993;Noguchi et al, 1993). Tissue culture and in vivo studies have shown that Schwann cells ensheathing regenerating neurites synthesize laminin, a basal lamina constituent and a promoter of neurite growth (Cornbrooks at al., 1983;Lander, 1987;Bunge et al, 1989).…”

Section: Introductionmentioning

confidence: 99%

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Vasoactive intestinal peptide: Mediator of laminin synthesis in cultured Schwann cells

et al. 1996

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To learn more about neuropeptide‐induced glial responses which accompany axon regeneration, we studied effects of VIP on laminin production by cultured Schwann cells. Schwann cells were isolated from sciatic nerves of neonatal mice, purified, and incubated for 5 days in either control medium (DMEM + 15% FCS) or control medium containing 10−7 ‐10−11 M VIP. At 10−7 and 10−8 M VIP, laminin levels measured by enzyme‐linked immunosorbent assay were significantly higher (55% and 35%) than those in control cultures. Lower VIP concentrations (10−9 ‐10−11 M) produced smaller increases which were not significant. Low‐affinity VIP receptors which mediated this effect were demonstrated on Schwann cells by radioligand binding studies. The increased Schwann cell synthesis of laminin induced by VIP was blocked when either a VIP antagonist or a VIP receptor antagonist was added to the VIP‐containing incubation medium. In contrast to astrocytes, when Schwann cells were loaded with fura‐2, VIP did not increase cytosolic Ca2+. This indicates that Schwann cells and astrocytes may have different intracellular transduction pathways; their receptor subtypes also may differ. We suggest that the VIP‐induced increase in laminin synthesis which we have observed in cultured Schwann cells may also occur in vivo and might be an important component of axon‐Schwann cell interactions during nerve regeneration. © 1996 Wiley‐Liss, Inc.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Vasoactive intestinal peptide: Mediator of laminin synthesis in cultured Schwann cells

et al. 1996

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“…Impaired nerve fiber regeneration is one of the salient pathological features in human diabetic neuropathy, and its correction is a major target for the therapeutic approach. In experimental diabetic animal models, extension of nerve bundles after transection and recovery of nerve function after crush or frozen injuries were delayed. Underlying mechanisms for the impaired fiber regeneration in diabetes have been ascribed to hyperglycemia‐related metabolic abnormalities, including the polyol pathway, glycation and oxidative stress.…”

Section: Introductionmentioning

confidence: 99%

Impaired nerve fiber regeneration in axotomized peripheral nerves in streptozotocin‐diabetic rats

Nishida

Yamagishi

Mizukami

et al. 2013

J of Diabetes Invest

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Aim/Introduction: Impaired nerve fiber regeneration is a salient feature of diabetic neuropathy. Its pathogenesis is still unclear. We attempted to characterize the structure of regenerated myelinated fibers after transection in streptozotocin-diabetic rats. Materials and Methods: Streptozotocin-diabetic rats underwent transection of the sciatic nerve. Two and 4 weeks postaxotomy, regenerated myelinated fibers of the cut end and fibers at its proximal site were morphometrically examined. Non-diabetic control rats with axotomy were also examined for comparison. Results: At 4 weeks post-axotomy, diabetic rats showed an increased myelinated fiber density and total fiber number with a trend toward reduced fiber size at the cut end compared with those in control rats. The average number of myelin lamellae relative to axonal size in regenerated fibers at the cut end was significantly reduced in diabetic rats compared with that in control rats. The proximal site showed a reduced size of fibers and axons in both diabetic and control rats to a similar extent compared with those in a non-axotomized state. At 2 weeks post-axotomy, these findings were less apparent. Conclusions: The nerves of diabetic rats when axotomized undergo impaired regeneration characterized by increased fiber density with hypomyelination. (J Diabetes Invest,

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“…After the experiments related to NCV, animals were anesthetized with 400 mg/kg chloral hydrate and following cardiac perfusion by using saline and bouin's fixative [34]. Then a section of sciatic nerve (1 cm) was removed and kept in bouin's fixative.…”

Section: Histopathological Evaluationmentioning

confidence: 99%

Neuroprotective Effect of Cerebrolysin on Diabetic Neuropathy: A Study on Male Rats

Mohtashami¹

2014

J Diabetes Metab

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Objective: Diabetes mellitus with 10% prevalence in human population leads to disorders of peripheral nervous system in many affected patients. It causes various polyneuropathies in which nerve conductionvelocity decreases. The aim of this study was to investigate the effect of cerebrolysinon the treatment of neural injuries resulted from hyperglycemia.Method: Diabetes was induced in male rats weighing 250 ± 25 gr by intraperitoneal injection of 65 mg/kg streptozocin (STZ). Six weeks after STZ injection and appearance of neuropathy in diabetic rats, animals were divided into four groups: experimental, vehicle, diabetic and control. The experimental and vehicle groups received respectively single dose of 5 mg/kg day -1 cerebrolysinand saline intraperitoneally for two weeks. At the end, in order to find the efficacy of cerebrolysin, all groups underwent behavioral and electrophysiological tests as well as histological investigation.Results: Metabolic parameters in different groups showed inefficacy of cerebrolysin in the treatment of metabolic disorders of diabetes. However, electrophysiological investigations showed efficacy of cerebrolysin in the treatment of diabetic neuropathy in rats. Moreover, investigation on morphologic structure of sciatic nerve was evident of the return of axon degenerative changes and myelin splitting in nerve fibers in cerebrolysin-received group. The results of behavioral studies showed increase in recovery in cerebrolysin group. Conclusion:According to the results, treatment of diabetic neuropathy with daily injection of 5 mg/kg cerebrolysin for two weeks improves rats' condition. J ou rna l o f D ia be tes & M e ta bolism

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Impaired induction of vasoactive intestinal polypeptide after sciatic nerve injury in the streptozotocin-diabetic rat

Cited by 13 publications

References 65 publications

Vasoactive intestinal peptide: Mediator of laminin synthesis in cultured Schwann cells

Vasoactive intestinal peptide: Mediator of laminin synthesis in cultured Schwann cells

Impaired nerve fiber regeneration in axotomized peripheral nerves in streptozotocin‐diabetic rats

Neuroprotective Effect of Cerebrolysin on Diabetic Neuropathy: A Study on Male Rats

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