Impedance spectroscopy of bilayer lipid membranes self-assembled on agar support – interaction with HDL

Legin, Michal; Laputková, Galina; Sabó, Ján; Vojcíková, L

doi:10.33549/physiolres.931305

Cited by 7 publications

(3 citation statements)

References 17 publications

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“…The measurements were performed in HEPES buffer solutions (pH 7.4) containing one of the following electroactive couples: Fe(CN) 6 3–/4– , Ru(NH 3 ) 6 3+/2+ and NAD + /NADH. In the case of the psBLMs, the measurements were performed in a normal three electrode system, with the working electrode (Au, 0.020 cm 2 ) being placed in one side of the psBLM and the reference (Ag/AgCl, 3.5 M KCl) and counter (Pt flag, 1 cm 2 ) being placed on the other side of the psBLM (Figure ). ,− Both chambers were filled with the same working solution.…”

Section: Methodsmentioning

confidence: 99%

Electron Transport in Supported and Tethered Lipid Bilayers Modified with Bioelectroactive Molecules

Campos

Kataky

2012

J. Phys. Chem. B

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Tethered bilayer lipid membranes (tBLMs) are commonly used as model membranes. However, in biophysical studies, free-standing membranes (“black” lipid membranes) are more realistic models of cellular processes. In this article, we discuss the rates of electron transfer in both types of bilayer lipid membranes. These BLMs were then modified using two very important mitochondrial membrane-associated molecules: ubiquinone-10 (UQ10) and α-tocopherol (VitE). The electron transfer rates in the unmodified films were studied with three redox couples, Fe(CN)6(3-/4-), Ru(NH3)6(3+/2+), and NAD+/NADH, using electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The rate of electron transfer in the modified films was studied using the biologically relevant NAD+/NADH electroactive couple using the same methods. It is shown that when the BLMs are modified with only UQ10, it is possible to observe electron transfer. However, when the antioxidant VitE is added to the modification, the electron transfer provided by UQ10 is inhibited. Following initial studies using CV, a comparison of electron transfer theory and data was used to investigate this phenomenon in more detail, using EIS data. The standard rate constant caused by electron tunneling across the film, k(th)(0), depends on the value of β used. Two different values of the potential independent electron tunneling coefficient, β, were fitted, and it is shown that a β value half of those usually reported in literature (refereed here as β(app)) gives better agreement between the theory and the experimental results. The unmodified films present k(th)(0) values on the order of 10(-15) cm s(-1) when β = 0.72 Å(-1) and k(th)(0) values on the order of 10(-9) cm s(-1) when β(app) = 0.38 Å(-1). For the modified films, the values of k(th)(0) are on the order of 10(-15) cm s(-1) when β = 0.72 Å(-1) and 10(-9) cm s(-1) for β(app) = 0.38 Å(-1). The experimental electron transfer rate constant, k(app)(0), is on the order of 10(-8) cm s(-1) for unmodified and modified (with (i) UQ10, (ii) VitE, and (iii) UQ10 + VitE) films.

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Section: Methodsmentioning

confidence: 99%

Electron Transport in Supported and Tethered Lipid Bilayers Modified with Bioelectroactive Molecules

Campos

Kataky

2012

J. Phys. Chem. B

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“…[ 18 ] Explicitly probing the response of multiple sinusoidal frequencies allows for analysis in frequency‐space, enabling deconvolution of electrical elements like capacitors and resistors. [ 16–18,21 ] Here, we utilize dEIS to disentangle the system's memristive and memcapacitive properties arising from molecular and conformational changes in the lipid bilayer. By separating these processes, we provide: (1) new insights into the design of new neuromorphic devices; (2) an understanding as to how to build multiple memelement functionalities into a single two‐terminal device; (3) demonstration of memristive behavior from dielectric loss change and not solely ion transport through the bilayer; and (4) a proof‐of‐principle demonstration for the use of dEIS in evaluating the next‐generation of biomimetic electronic devices.…”

Section: Introductionmentioning

confidence: 99%

“…On the other hand, dEIS interrogates the temporal development of kinetics and dynamics in electrochemical systems, such as DIBs, by applying a multisine probe to monitor changes in the frequency-resolved electrical responses (impedance) as functions of time. [15][16][17][18] Analysis of the dynamic complex impedance spectra enables observation of time-dependent capacitance and resistance changes that give rise to the pinched hysteresis observed in these memelements.…”

Section: Introductionmentioning

confidence: 99%

Disentangling Memristive and Memcapacitive Effects in Droplet Interface Bilayers Using Dynamic Impedance Spectroscopy

Sacci

Scott

Liu

et al. 2022

Adv Elect Materials

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The underlying principles for generating intelligent behavior in living organisms are fundamentally different from those in traditional solid‐state circuits. Biomimetic neuromorphic equivalents based on biological membranes offer novel implementation of tunable plasticity and diverse mechanisms to control functionality. Here, dynamic electrochemical impedance spectroscopy (dEIS) to probe diphytanoylphosphatidylcholine (DPhPC) droplet interface bilayers (DIBs) to better understand the differences in molecular level structure/dynamics that give rise to hysteretic loops and neuromorphic, memelement behaviors in lipid bilayers in response to electrical biasing is used. Importantly, this system does not have ion‐conducting channels and is, therefore, not expected to show memristive behavior. Surprisingly, both memristive and memcapacitive behaviors by measuring the time‐dependent complex impedance of DPhPC DIBs are detected. It is shown that nonlinear memristance can originate from structural changes in the bilayer, affecting its dielectric properties. This novel dEIS application allows for the simultaneous analysis of the system's changing memristive and memcapacitive properties, which originate from different molecular restructuring processes. Moreover, and importantly, access to this type of information increases the number of neuromorphic processes supported simultaneously in a single two‐terminal device.

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Layer‐by‐Layer Synthesis of Thick Mesoporous TiO₂ Films with Vertically Oriented Accessible Nanopores and Their Application for Lithium‐Ion Battery Negative Electrodes

Nagpure

Khan

Islam

et al. 2018

Adv Funct Materials

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TiO 2 films of varying thicknesses (up to ≈1.0 µm) with vertically oriented, accessible 7-9 nm nanopores are synthesized using an evaporation-induced self-assembly layer-by-layer technique. The hypothesis behind the approach is that epitaxial alignment of hydrophobic blocks of surfactant templates induces a consistent, accessible mesophase orientation across a multilayer film, ultimately leading to continuous, vertically aligned pore channels. Characterization using grazing incidence X-ray scattering, scanning electron microscopy, and impedance spectroscopy indicates that the pores are oriented vertically even in relatively thick films (up to 1 µm). These films contain a combination of amorphous and nanocrystalline anatase titania of value for electrochemical energy storage. When applied as negative electrodes in lithium-ion batteries, a capacity of 254 mAh g −1 is obtained after 200 cycles for a single-layer TiO 2 film prepared on modified substrate, higher than on unmodified substrate or nonporous TiO 2 film, due to the high accessibility of the vertically oriented channels in the films. Thicker films on modified substrate have increased absolute capacity because of higher mass loading but a reduced specific capacity because of transport limitations. These results suggest that the multilayer epitaxial approach is a viable way to prepare high capacity TiO 2 films with vertically oriented continuous nanopores.insertion of Li + between two electrodes with simultaneous removal and addition of electrons to store or release electrical energy. [2] However, the performance of LIBs depends strongly on the electrode materials and their interaction with the electrolyte. [3] Although many high capacity LIB negative electrode materials are available including Si, Ge, and Sn, they cannot be used in bulk form due to their low range of operating voltage, high stress generated by intercalation of Li + , and the consumption of Li + by unstable solid-electrolyte interface layers during cycling. [4] TiO 2 is a suitable candidate for negative electrodes in LIBs for applications requiring high rate performance and high electrolyte solution stability. [5] Although the theoretical capacity of TiO 2 (330 mAh g −1 ) [6] is lower than that of Sn, Si, or graphite, TiO 2 negative electrodes offer better safety, [6,7] which is one of the major criteria for practical use of batteries. [1b] The relatively high Li + discharge voltage plateau (1.7 V) of TiO 2 avoids the formation of solid-electrolyte interphase layers and electroplating of Li + during cycling. [8] TiO 2 also possesses low volume expansion during Li + insertion, and therefore low strain, which makes it less susceptible to structural deformation and the associated loss of performance on cycling. [6] However, the major barrier in using bulk TiO 2 in LIBs is its poor Li + ionic and electrical Nanoporous Films [+] Present address: Intel Corp.,

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Impedance spectroscopy of bilayer lipid membranes self-assembled on agar support – interaction with HDL

Cited by 7 publications

References 17 publications

Electron Transport in Supported and Tethered Lipid Bilayers Modified with Bioelectroactive Molecules

Electron Transport in Supported and Tethered Lipid Bilayers Modified with Bioelectroactive Molecules

Disentangling Memristive and Memcapacitive Effects in Droplet Interface Bilayers Using Dynamic Impedance Spectroscopy

Layer‐by‐Layer Synthesis of Thick Mesoporous TiO₂ Films with Vertically Oriented Accessible Nanopores and Their Application for Lithium‐Ion Battery Negative Electrodes

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Impedance spectroscopy of bilayer lipid membranes self-assembled on agar support – interaction with HDL

Cited by 7 publications

References 17 publications

Electron Transport in Supported and Tethered Lipid Bilayers Modified with Bioelectroactive Molecules

Electron Transport in Supported and Tethered Lipid Bilayers Modified with Bioelectroactive Molecules

Disentangling Memristive and Memcapacitive Effects in Droplet Interface Bilayers Using Dynamic Impedance Spectroscopy

Layer‐by‐Layer Synthesis of Thick Mesoporous TiO2 Films with Vertically Oriented Accessible Nanopores and Their Application for Lithium‐Ion Battery Negative Electrodes

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Product

Resources

About

Layer‐by‐Layer Synthesis of Thick Mesoporous TiO₂ Films with Vertically Oriented Accessible Nanopores and Their Application for Lithium‐Ion Battery Negative Electrodes