Impedance spectroscopy using maximum length sequences: Application to single cell analysis

Gawad, Shady; Sun, Tao; Green, Nicolas G.; Morgan, Hywel

doi:10.1063/1.2737751

Cited by 51 publications

(37 citation statements)

References 36 publications

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“…Though its robustness and high accuracy results, it is still more common in laboratory tests than in online field equipment. [6], [7] and [8] investigate identification methods based on broadband signals. [6] aims to identify biological impedance while [7] and [8] work on battery impedance measurements.…”

Section: Introductionmentioning

confidence: 99%

Classical EIS and square pattern signals comparison based on a well-known reference impedance

Nazer

Cattin

Granjon

et al. 2013

2013 World Electric Vehicle Symposium and Exhibition (EVS27)

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Section: Introductionmentioning

confidence: 99%

Classical EIS and square pattern signals comparison based on a well-known reference impedance

Nazer

Cattin

Granjon

et al. 2013

2013 World Electric Vehicle Symposium and Exhibition (EVS27)

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“…In contrast to measurements of single cells at discrete frequencies, Morgan et al have developed a method for broad band multifrequency analysis of cells using pseudorandom signal generated by a maximum length sequence (MLS) system (76)(77)(78)(79)(80). This method permits the characterization of cells within 1 ms at 512 discrete frequencies ranging from 976 Hz to 500 kHz.…”

Section: Single Cell Dielectric Spectroscopy: Multifrequency Analysismentioning

confidence: 99%

Microfluidic impedance‐based flow cytometry

Berardino²,

et al. 2010

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Microfabricated flow cytometers can detect, count, and analyze cells or particles using microfluidics and electronics to give impedance-based characterization. Such systems are being developed to provide simple, low-cost, label-free, and portable solutions for cell analysis. Recent work using microfabricated systems has demonstrated the capability to analyze micro-organisms, erythrocytes, leukocytes, and animal and human cell lines. Multifrequency impedance measurements can give multiparametric, high-content data that can be used to distinguish cell types. New combinations of microfluidic sample handling design and microscale flow phenomena have been used to focus and position cells within the channel for improved sensitivity. Robust designs will enable focusing at high flowrates while reducing requirements for control over multiple sample and sheath flows. Although microfluidic impedance-based flow cytometers have not yet or may never reach the extremely high throughput of conventional flow cytometers, the advantages of portability, simplicity, and ability to analyze single cells in small populations are, nevertheless, where chip-based cytometry can make a large impact. ' 2010International Society for Advancement of Cytometry Key terms microfluidics; impedance characterization; label free; single cell analysis; hydrodynamic focusing; sorting MICROFLUIDIC flow cytometers can bring many advantages to the field of flow cytometry (FCM). Compared to typical flow cytometry channel sizes, the miniaturized dimensions permit microfluidic systems to analyze single cells, to identify cellular variability in gene expression, or drug response within a cell population. Chipbased cytometers can have lower size and costs than conventional benchtop instruments, and may be portable. Today, the developmental aim for microfluidic systems is to reach the same sensitivity and capability for multiparametric analyses as delivered by conventional flow cytometers. Many efforts have been made to improve existing devices and to create new miniaturized high-end instruments. Microfluidic chips can incorporate on-chip cell preparation, cell culture, lysis, and modules for optical, electrophoretic, or genomic analysis (1). They are also suitable for analysis of cells in suspension as well as adherent cells.Miniaturized cytometry devices will have high impact in the development of point-of-care devices in developing countries. Accurate CD4 1 T-cell counts are used to monitor human immunodeficiency virus (HIV)-infected patients, and various thresholds of the number of CD4 1 T lymphocytes per ll of whole blood are used to start antiretroviral therapy (2-5). A simple, single-purpose CD4 cell counting device, which does not require standard laboratory equipment or trained laboratory personnel, could help some of the 33 million HIV-infected people worldwide monitor the stage of infection (6)(7)(8). In this application, increased analysis throughput is a secondary concern compared to increased sensitivity and specificity. Portable, miniatu...

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“…For that reason, several types of broadband excitation signals (e.g., Maximal Length Sequence (Gawad et al, 2007), multisine (Sanchez et al 2011)) are preferable. However, in this chapter, we will focus on the use of chirp excitation due to several advantages of it: wide and flat amplitude spectrum (Nahvi & Hoyle, 2009) together with independent scalability in the time and frequency domain.…”

Section: Fig 1 Formation Of the Electrical Bioimpedance Of Tissuementioning

confidence: 99%