Impedimetric ultrasensitive detection of trypsin based on hybrid aptamer-2DMIP using a glassy carbon electrode modified by nickel oxide nanoparticle

Roushani, Mahmoud; Zalpour, Neda

doi:10.1016/j.microc.2021.106955

Cited by 29 publications

(6 citation statements)

References 47 publications

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“…They can be synthesized in the presence of a template (analyte) and are a promising alternative to biomolecules in the design of biosensors [119]. Since the principle of creating biosensors based on MIPs differs from the immobilization of working electrodes based on other materials, including polymers [113,119,[123][124][125][126][127], in this section, we highlight MIPs as a material for electrode modification.…”

Section: Polymersmentioning

confidence: 99%

Hybrid Impedimetric Biosensors for Express Protein Markers Detection

Sitkov,

Ryabko,

Moshnikov

et al. 2024

Micromachines

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Impedimetric biosensors represent a powerful and promising tool for studying and monitoring biological processes associated with proteins and can contribute to the development of new approaches in the diagnosis and treatment of diseases. The basic principles, analytical methods, and applications of hybrid impedimetric biosensors for express protein detection in biological fluids are described. The advantages of this type of biosensors, such as simplicity and speed of operation, sensitivity and selectivity of analysis, cost-effectiveness, and an ability to be integrated into hybrid microfluidic systems, are demonstrated. Current challenges and development prospects in this area are analyzed. They include (a) the selection of materials for electrodes and formation of nanostructures on their surface; (b) the development of efficient methods for biorecognition elements’ deposition on the electrodes’ surface, providing the specificity and sensitivity of biosensing; (c) the reducing of nonspecific binding and interference, which could affect specificity; (d) adapting biosensors to real samples and conditions of operation; (e) expanding the range of detected proteins; and, finally, (f) the development of biosensor integration into large microanalytical system technologies. This review could be useful for researchers working in the field of impedimetric biosensors for protein detection, as well as for those interested in the application of this type of biosensor in biomedical diagnostics.

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Section: Polymersmentioning

confidence: 99%

Hybrid Impedimetric Biosensors for Express Protein Markers Detection

Sitkov,

Ryabko,

Moshnikov

et al. 2024

Micromachines

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“…The limit of detection of the MIP–aptamer was over half (2.4 nm) that of MIPs (4.1 nm). In addition, Roushani et al [ 55 ] detected ultra-trace trypsin in human serum and urine by using a MIP–aptamer. Satisfactory results were obtained with recoveries ranging from 94.0 to 114.0%.…”

Section: Application Of Mip–aptamer In the Molecular Recognition Of C...mentioning

confidence: 99%

Molecularly Imprinting–Aptamer Techniques and Their Applications in Molecular Recognition

Zhou

Liu

2022

Biosensors

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Molecular imprinting–aptamer techniques exhibit the advantages of molecular imprinting and aptamer technology. Hybrids of molecularly imprinted polymer–aptamer (MIP–aptamer) prepared by this technique have higher stability, binding affinity and superior selectivity than conventional molecularly imprinted polymers or aptamers. In recent years, molecular imprinting–aptamer technologies have attracted considerable interest for the selective recognition of target molecules in complex sample matrices and have been used in molecular recognition such as antibiotics, proteins, viruses and pesticides. This review introduced the development of molecular imprinting–aptamer-combining technologies and summarized the mechanism of MIP–aptamer formation. Meanwhile, we discussed the challenges in preparing MIP–aptamer. Finally, we summarized the application of MIP–aptamer to the molecular recognition in disease diagnosis, environmental analysis, food safety and other fields.

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“…A simple and effective strategy to potentially solve this problem is a double recognition strategy involving MIP and aptamer. Roushani et al, in particular, have advanced this field and used such a strategy to detect small molecules, proteins, and viruses. − For example, a nanohybrid probe was constructed based on double recognition by an aptamer-based MIP grafted onto a multiwalled carbon nanotubes-chitosan nanocomposites; when this probe was used to detect the core antigen of hepatitis C virus, it reached an impressive detection limit of 1.67 fg mL –1 . Similarly, an artificial receptor biosensor for the recognition of ultratrace trypsin was designed using a hybrid method including both MIP and aptamer technologies.…”

Section: Introductionmentioning

confidence: 99%

“…Similarly, an artificial receptor biosensor for the recognition of ultratrace trypsin was designed using a hybrid method including both MIP and aptamer technologies. The investigation of imprinting factors with or without aptamers showed that the inclusion of aptamer technology significantly increased the throughput of the sensor . By introducing aptamer strings and MIP, a dual-recognition biosensor was also constructed to selectively detect intact SARS-CoV-2 virus.…”

Section: Introductionmentioning

confidence: 99%

“…The investigation of imprinting factors with or without aptamers showed that the inclusion of aptamer technology significantly increased the throughput of the sensor. 28 By introducing aptamer strings and MIP, a dual-recognition biosensor was also constructed to selectively detect intact SARS-CoV-2 virus. This sensor was found to exhibit a higher selectivity than single-element sensors.…”

Section: ■ Introductionmentioning

confidence: 99%

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Non-autofluorescence Detection of H5N1 Virus Using Photochemical Aptamer Sensors Based on Persistent Luminescent Nanoparticles

Chen

Cai

Gong³

et al. 2022

ACS Appl. Mater. Interfaces

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Fluorescence sensing is limited in practical applications owing to multiple autofluorescent substances in complex biological samples such as serum. In this paper, the luminescence decay effect of persistent luminescent nanoparticles (PLNPs) was used to avoid the interference of autofluorescence in complex biological samples, and a non-autofluorescence molecularly imprinted polymer aptamer sensor (MIP-aptasensor) was designed to detect H5N1 virus. The proposed MIP-aptasensor consists of a magnetic MIP and aptamer-functionalized persistent luminescent nanoparticle Zn2GeO4:Mn2+-H5N1 aptamer (ZGO-H5N1 Apt). Upon simultaneous recognition of H5N1 virus, strong persistent luminescent signal changes were produced. Using the unique luminescent characteristics of PLNPs and the high selectivity of imprinted polymers and aptamers, the designed MIP-aptasensor effectively eliminates the autofluorescence background interference of serum samples and realizes the non-autofluorescence detection of H5N1 virus with high sensitivity (a limit of detection of 0.0128 HAU mL–1, 1.16 fM) and selectivity (the imprinting factor for the target H5N1 virus was 6.72). This tool provides a strategy for the design of sensors and their application in complex biological samples.

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Impedimetric ultrasensitive detection of trypsin based on hybrid aptamer-2DMIP using a glassy carbon electrode modified by nickel oxide nanoparticle

Cited by 29 publications

References 47 publications

Hybrid Impedimetric Biosensors for Express Protein Markers Detection

Hybrid Impedimetric Biosensors for Express Protein Markers Detection

Molecularly Imprinting–Aptamer Techniques and Their Applications in Molecular Recognition

Non-autofluorescence Detection of H5N1 Virus Using Photochemical Aptamer Sensors Based on Persistent Luminescent Nanoparticles

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