Imperative role of dental pulp stem cells in regenerative therapies: A systematic review

Kabir, Ramchandra; Gupta, Manish; Aggarwal, Avanti; Sharma, Deepak; Sarin, Anurag; Kola, Mohammed Zaheer

doi:10.4103/1117-6806.127092

Cited by 38 publications

References 73 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Generation of functional hepatocyte-like cells from human deciduous periodontal ligament stem cells

Vasanthan

Jayaraman

Kunasekaran

et al. 2016

Sci Nat

View full text Add to dashboard Cite

Human deciduous periodontal ligament stem cells have been introduced for as an easily accessible source of stem cells from dental origin. Although recent studies have revealed the ability of these stem cells in multipotential attribute, their efficiency of hepatic lineage differentiation has not been addressed so far. The aim of this study is to investigate hepatic lineage fate competence of periodontal ligament stem cells through direct media induction. Differentiation of periodontal ligament stem cells into hepatocyte-like cells was conducted by the exposure of two phase media induction. First phase was performed in the presence of hepatocyte growth factors to induce a definitive endoderm formation. In the subsequent phase, the cells were treated with oncostatin M and dexamethosone followed by insulin and transferrin to generate hepatocyte-like cells. Hepatic-related characters of the generated hepatocyte-like cells were determined at both mRNA and protein level followed by functional assays. Foremost changes observed in the generation of hepatocyte-like cells were the morphological features in which these cells were transformed from fibroblastic shape to polygonal shape. Temporal expression of hepatic markers ranging from early endodermal up to late markers were detected in the hepatocyte-like cells. Crucial hepatic markers such as glycogen storage, albumin, and urea secretion were also shown. These findings exhibited the ability of periodontal ligament stem cells of dental origin to be directed into hepatic lineage fate. These cells can be regarded as an alternative autologous source in the usage of stem cell-based treatment for liver diseases.

show abstract

Generation of functional hepatocyte-like cells from human deciduous periodontal ligament stem cells

Vasanthan

Jayaraman

Kunasekaran

et al. 2016

Sci Nat

View full text Add to dashboard Cite

show abstract

CD146 Expression Influences Periapical Cyst Mesenchymal Stem Cell Properties

Paduano

Marrelli

Palmieri

et al. 2016

Stem Cell Rev and Rep

View full text Add to dashboard Cite

Recent studies have identified a new human dental derived progenitor cell population with multi-lineage differentiation potential referred to as human periapical cyst mesenchymal stem cells (hPCy-MSCs). In the present study, we compared two subpopulations of hPCy-MSCs characterised by the low or high expression of CD146 to establish whether this expression can regulate their stem cell properties. Using flow cytometry, we evaluated the stem cell marker profile of hPCy-MSCs during passaging. Furthermore, CD146 and CD146 cells were sorted by magnetic beads and subsequently both cell populations were evaluated for differences in their proliferation, self-renewal, stem cell surface markers, stemness genes expression and osteogenic differentiation potential.We found that hPCy-MSCs possessed a stable expression of several mesenchymal stem cell surface markers, whereas CD146 expression declined during passaging.In addition, sorted CD146 cells proliferated significantly faster, displayed higher colony-forming unit-fibroblast capacity and showed higher expression of Klf4 when compared to the CD146 subset. Significantly, the osteogenic potential of hPCy-MSCs was greater in the CD146 than in CD146 population. These results demonstrate that CD146 is spontaneously downregulated with passaging at both mRNA and protein levels and that the high expression of CD146 reduces the proliferative, self-renewal and osteogenic differentiation potential of hPCy-MSCs. In conclusion, our study demonstrates that changes in the expression of CD146 can influence the stem cell properties of hPCy-MSCs.

show abstract

Regenerative Potential of DPSCs and Revascularization: Direct, Paracrine or Autocrine Effect?

Mattei

Martellucci

Pulcini

et al. 2021

Stem Cell Rev and Rep

View full text Add to dashboard Cite

A new source of mesenchymal stem cells has recently been discovered, the so-called dental pulp derived stem cells (DPSCs) which therefore could represent potentially tools for regenerative medicine. DPSC originate from the neural crest and are physiologically involved in dentin homeostasis; moreover, they contribute to bone remodeling and differentiation into several tissues including cartilage, bone, adipose and nervous tissues. DPSCs have also been shown to influence the angiogenesis process, for example through the release of secretory factors or by differentiating into vascular and/or perivascular cells. Angiogenesis, that has a pivotal role in tissue regeneration and repair, is defined as the formation of new vessels from preexisting vessels and is mediated by mutual and reciprocal interactions between endothelial cells and perivascular cells. It is also known that co-cultures of perivascular and endothelial cells (ECs) can form a vascular network in vitro and also in vivo. Since DPSCs seem to have characteristics similar to pericytes, understanding the possible mechanism of interaction between DPSCs and ECs during neo-angiogenesis is dramatically important for the development of advanced clinical application in the field of regeneration. Graphical abstract

show abstract

Imperative role of dental pulp stem cells in regenerative therapies: A systematic review

Cited by 38 publications

References 73 publications

Generation of functional hepatocyte-like cells from human deciduous periodontal ligament stem cells

Generation of functional hepatocyte-like cells from human deciduous periodontal ligament stem cells

CD146 Expression Influences Periapical Cyst Mesenchymal Stem Cell Properties

Regenerative Potential of DPSCs and Revascularization: Direct, Paracrine or Autocrine Effect?

Contact Info

Product

Resources

About