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Background. This study aimed to investigate the endodontic debridement efficacy of different sodium hypochlorite (NaOCl) irrigation regimens with and without ultrasonic agitation, followed by ethylenediaminetetraacetic acid (EDTA) via scanning electron microscopy (SEM) after using a rotary instrumentation system. Methods. Mandibular premolars (n=50) were randomly divided into five experimental groups (n=10) for root canal instrumentation with ProTaper Universal rotary system up to F3. The root canal system was treated with intracanal-heated NaOCl (100°C) or preheated NaOCl (55°C), followed by ultrasonic agitation and EDTA treatment. Samples irrigated with conventional needle irrigation (CNI) using normal saline solution were used as controls. Debridement efficacy was analyzed by SEM. A five-point scale was used to estimate the presence/absence of debris for each canal segment (coronal, middle, and apical). The results were analyzed using one-way ANOVA and post hoc Tukey tests (P<0.05). Results. The experimental groups exhibited less debris compared to CNI with saline (P<0.05). The amount of debris decreased significantly for the group with NaOCl intracanal heating compared to extraoral heating. Ultrasonic agitation further enhanced the root canal debridement efficacy of NaOCl. Conclusion. In summary, intracanal heating of NaOCl with and without ultrasonic agitation followed by EDTA appears to be a promising method to flush debris from the root canal system.

Aim: This study aimed to evaluate the influence of humidity on the bond strength of AH Plus, BioRoot RCS, and Nanoseal-S sealers. Materials and Methods: Sixty root slices, each 1 ± 0.1 mm thick, were prepared from the middle-third of 20 mandibular premolars. Three holes, 0.8 mm wide each, were drilled on each slice. These holes were subjected to standardized irrigations and dried. Each hole in each slice was filled with the three individual sealers, AH Plus, BioRoot RCS, and Nanoseal-S. The samples were then stored in saline for 7 days at 37°C. The push-out bond strength of each sealer in each hole of all samples was tested. The differences in push-out bond strengths between the three sealer samples were assessed using the unpaired t-test and post hoc Turkey's HSD test. The significance level was set at 5%. Results: The results of the post hoc Tukey's HSD test (P < 0.05) indicated that the mean and median push-out bond strength were highest for BioRoot RCS, irrespective of the moisture conditions. Conclusion: Humidity conditions did not influence the bond strength of AH Plus, BioRoot RCS, and Nanoseal-S sealers.

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