Implant size and mechanical properties influence the failure of the adhesive bond between cartilage implants and native tissue in a finite element analysis

Vahdati, Akbar; Wagner, Diane R.

doi:10.1016/j.jbiomech.2013.03.019

Cited by 33 publications

(24 citation statements)

References 38 publications

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“…Early implant loss and delamination are major challenges when fixing cartilage tissue engineering implants in cartilage defects via adhesives . Considering the central mechanical function of cartilage, early restoration of the physical hardness by stimulating calcification at the bottom of the repair tissue seems highly favorable.…”

Section: Discussionmentioning

confidence: 99%

Stimulation of a calcified cartilage connecting zone by GDF‐5‐augmented fibrin hydrogel in a novel layered ectopic in vivo model

et al. 2017

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Tissue engineering approaches for reconstructing full-depth cartilage defects need to comprise a zone of calcified cartilage to tightly anchor cartilage constructs into the subchondral bone. Here, we investigated whether growth and differentiation factor-5-(GDF-5)-augmented fibrin hydrogel can induce a calcified cartilage-layer in vitro that seamlessly connects cartilage-relevant biomaterials with bone tissue. Human bone marrow stromal cells (BMSCs) were embedded in fibrin hydrogel and subjected to chondrogenesis with TGF-β with or without GDF-5 before constructs were implanted subcutaneously into SCID mice. A novel layered ectopic in vivo model was developed and GDF-5-augmented fibrin with BMSCs was used to glue hydrogel and collagen constructs onto bone disks to investigate formation of a calcified cartilage connecting zone. GDF-5 significantly enhanced ALP activity during in vitro chondrogenesis while ACAN and COL2A1 mRNA, proteoglycan-, collagen-type-II- and collagen-type-X-deposition remained similar to controls. Pellets pretreated with GDF-5 mineralized faster in vivo and formed more ectopic bone. In the novel layered ectopic model, GDF-5 strongly supported calcified cartilage formation that seamlessly connected with the bone. Pro-chondrogenic and pro-hypertrophic activity makes GDF-5-augmented fibrin an attractive bioactive hydrogel with high potential to stimulate a calcified cartilage connecting zone in situ that might promote integration of cartilage scaffolds with bone. Thus, GDF-5-augmented fibrin hydrogel promises to overcome poor fixation of biomaterials in cartilage defects facilitating their long-term regeneration. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 2214-2224, 2018.

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Section: Discussionmentioning

confidence: 99%

Stimulation of a calcified cartilage connecting zone by GDF‐5‐augmented fibrin hydrogel in a novel layered ectopic in vivo model

et al. 2017

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“…Normal articular cartilage can bear many millions of cycles of high force loading [3], but with exercise of increasing energy and force, the problem of aging becomes increasingly serious. Approximately 65% of the total population had suffered injury to their articular cartilage [4]. Because of the lack of blood vessels, nerves, and lymphatic tissue, cartilage cannot easily repair itself after injury.…”

Section: Introductionmentioning

confidence: 99%

“…Because of the lack of blood vessels, nerves, and lymphatic tissue, cartilage cannot easily repair itself after injury. In recent years, tissue engineering techniques to repair articular cartilage defects have attracted much attention [4, 5]. This technology is expected to be the most effective method to cure the cartilage defect.…”

Section: Introductionmentioning

confidence: 99%

Study of the Mechanical Environment of Chondrocytes in Articular Cartilage Defects Repaired Area under Cyclic Compressive Loading

Liu

Duan

Zhang

et al. 2017

Journal of Healthcare Engineering

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COMSOL finite element software was used to establish a solid-liquid coupling biphasic model of articular cartilage and a microscopic model of chondrocytes, using modeling to take into account the shape and number of chondrocytes in cartilage lacuna in each layer. The effects of cyclic loading at different frequencies on the micromechanical environment of chondrocytes in different regions of the cartilage were studied. The results showed that low frequency loading can cause stress concentration of superficial chondrocytes. Moreover, along with increased frequency, the maximum value of stress response curve of chondrocytes decreased, while the minimum value increased. When the frequency was greater than 0.2 Hz, the extreme value stress of response curve tended to be constant. Cyclic loading had a large influence on the distribution of liquid pressure in chondrocytes in the middle and deep layers. The concentration of fluid pressure changed alternately from intracellular to peripheral in the middle layer. Both the range of liquid pressure in the upper chondrocytes and the maximum value of liquid pressure in the lower chondrocytes in the same lacunae varied greatly in the deep layer. At the same loading frequency, the elastic modulus of artificial cartilage had little effect on the mechanical environment of chondrocytes.

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“…The large swelling ratios generally associated with these low modulus single‐component hydrogels are also a major concern for an implantable load‐bearing scaffold. Large changes in the hydrogel dimensions could lead to altered mechanical properties and cell behavior, and a higher likelihood of implant failure with the surrounding tissue …”

Section: Introductionmentioning

confidence: 99%

“…Large changes in the hydrogel dimensions could lead to altered mechanical properties and cell behavior, and a higher likelihood of implant failure with the surrounding tissue. [25][26][27] Blending of prepolymers has been used to incorporate complementary materials that can enhance the viability and biosynthesis of encapsulated cells. For example, chondroitin sulfate, when blended and cross-linked into either poly(ethylene glycol) (PEG) or poly(vinyl alcohol) (PVA)-based hydrogels, improved the overall modulus and acted as a degradable linkage that also promoted higher cell proliferation and matrix accumulation during culture.…”

Section: Introductionmentioning

confidence: 99%

Chondrocyte Generation of Cartilage‐Like Tissue Following Photoencapsulation in Methacrylated Polysaccharide Solution Blends

Hayami

Waldman

Amsden

2016

Macromolecular Bioscience

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Chondrocyte-seeded, photo-cross-linked hydrogels prepared from solutions containing 50% mass fractions of methacrylated glycol chitosan or methacrylated hyaluronic acid (MHA) with methacrylated chondroitin sulfate (MCS) are cultured in vitro under static conditions over 35 d to assess their suitability for load-bearing soft tissue repair. The photo-cross-linked hydrogels have initial equilibrium moduli between 100 and 300 kPa, but only the MHAMCS hydrogels retain an approximately constant modulus (264 ± 5 kPa) throughout the culture period. Visually, the seeded chondrocytes in the MHAMCS hydrogels are well distributed with an apparent constant viability in culture. Multicellular aggregates are surrounded by cartilaginous matrix, which contain aggrecan and collagen II. Thus, co-cross-linked MCS and MHA hydrogels may be suited for use in an articular cartilage or nucleus pulposus repair applications.

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Implant size and mechanical properties influence the failure of the adhesive bond between cartilage implants and native tissue in a finite element analysis

Cited by 33 publications

References 38 publications

Stimulation of a calcified cartilage connecting zone by GDF‐5‐augmented fibrin hydrogel in a novel layered ectopic in vivo model

Stimulation of a calcified cartilage connecting zone by GDF‐5‐augmented fibrin hydrogel in a novel layered ectopic in vivo model

Study of the Mechanical Environment of Chondrocytes in Articular Cartilage Defects Repaired Area under Cyclic Compressive Loading

Chondrocyte Generation of Cartilage‐Like Tissue Following Photoencapsulation in Methacrylated Polysaccharide Solution Blends

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