Implantation of canine umbilical cord blood-derived mesenchymal stem cells mixed with beta-tricalcium phosphate enhances osteogenesis in bone defect model dogs

Abstract

This study was performed to evaluate the osteogenic effect of allogenic canine umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) mixed with beta-tricalcium phosphate (β-TCP) in orthotopic implantation. Seven hundred milligrams of β-TCP mixed with 1 × 106 UCB-MSCs diluted with 0.5 ml of saline (group CM) and mixed with the same volume of saline as control (group C) were implanted into a 1.5 cm diaphyseal defect and wrapped with PLGC membrane in the radius of Beagle dogs. Radiographs of the antebrachium were made after surgery. The implants were harvested 12 weeks after implantation and specimens were stained with H&E, toluidine blue and Villanueva-Goldner stains for histological examination and histomorphometric analysis of new bone formation. Additionally, UCB-MSCs were applied to a dog with non-union fracture. Radiographically, continuity between implant and host bone was evident at only one of six interfaces in group C by 12 weeks, but in three of six interfaces in group CM. Radiolucency was found only near the bone end in group C at 12 weeks after implantation, but in the entire graft in group CM. Histologically, bone formation was observed around β-TCP in longitudinal sections of implant in both groups. Histomorphometric analysis revealed significantly increased new bone formation in group CM at 12 weeks after implantation (p < 0.05). When applied to the non-union fracture, fracture healing was identified by 6 weeks after injection of UCB-MSCs. The present study indicates that a mixture of UCB-MSCs and β-TCP is a promising osteogenic material for repairing bone defects.

Evaluation of the role of autogenous bone-marrow–derived mesenchymal stem cell transplantation for the repair of mandibular bone defects in rabbits

“…This increased osteogenesis could be attributable mainly to conversion of mesenchymal stem cells into osteoblasts, as reported by Jiang et al (Jiang et al, 2010). Also, Jang et al (Jang et al, 2008) recorded increased osteogenesis and new bone formation through differentiation of MSCs to osteoblast progenitors when tricalcium phosphate (TCP) was loaded onto canine umbilical cordederived mesenchymal stem cells.…”

Prospective Isolation of Mesenchymal Stem Cells from Multiple Mammalian Species Using Cross-Reacting Anti-Human Monoclonal Antibodies

“…For the initial screening of cultured MSCs from the nonhuman species, we used a panel of 43 mAb that contained the following commercially available mAb against human CD3,13,14,19,29,31,34,44,45,49,55,71,73,90,117,146, HLA-ABC, HLA-DR, Sca-1 [45] (Becton Dickinson), CD105 (Ancell Corp.), CD235a (Dako), CD271 (Clone 20.4 against low-affinity nerve growth factor receptor-allophycocyanin; Miltenyi Biotec), ALP (clone B4-78-c; Hybridoma Bank), KDR (R&D Systems), SSEA-4 (clone MC-813-70; Hybridoma Bank), W8B2 against human MSCs antigen-1 (MSCA-1= W8B2-phycoerythrin (PE); Biolegend), W4A5 against MSCs, and neural progenitor cells (W4A5-PE; Biolegend). Additionally, we used a series of mAb that showed reactivity with the CD271 þ subpopulation in human BM with putative MSCs specificity [46].…”

“…In orthopedic research, MSCs from goat [6][7][8][9][10][11], dog [12][13][14][15], sheep [16][17][18][19], and pig [20,21] are studied with the aim to correct defects of cartilage and bone. In cardiac research MSCs from dog [22][23][24], pig [25,26], and sheep [27,28] are studied for heart valve reconstruction and for cellular therapy to treat myocardial infarctions.…”

Mesenchymal Stem Cell-Based Bone Engineering for Bone Regeneration

“…For example, the construct made of porous cylindrical PLA scaffolds and autologus adipose-derived stem cells have been reported to successfully regenerate skull defects in New Zealand white rabbits (Bella et al, 2008). Similarly, Jang et al have noted the efficiency of construct comprised of beta TCPs and canine umbilical cord blood MSCs in canine cortical defect regeneration (Jang et al, 2008).…”