Implantation of green tea catechin α‐tricalcium phosphate combination enhances bone repair in rat skull defects

Rodriguez, Reena; Kondo, Hisamoto; Nyan, Myat; Jia, Hao; Miyahara, Takashige; Ohya, Keiichi; Kasugai, Shohei

doi:10.1002/jbm.b.31848

Cited by 46 publications

(42 citation statements)

References 54 publications

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“…In addition, EGCG has been demonstrated to improve the bone microstructure and quality in aged, orchidectomized rats (17). Rodriguez et al (18) found that a combination of α-tricalcium phosphate particles and EGCG effectively stimulated bone regeneration. Furthermore, in vitro tests revealed that EGCG increased the formation of mineralized bone nodules by human osteoblast-like cells (19).…”

Section: Introductionmentioning

confidence: 99%

Role of (-)-epigallocatechin-3-gallate in the osteogenic differentiation of human bone marrow mesenchymal stem cells: An enhancer or an inducer?

Jin

et al. 2015

Experimental and Therapeutic Medicine

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Epidemiological investigations have revealed that the consumption of green tea, which is a rich source of (-)-epigallocatechin-3-gallate (EGCG), is associated with a reduced risk of osteoporosis. A number of in vitro and in vivo studies have also demonstrated that EGCG exerts a significant positive effect on osteogenesis; however, the single effect of EGCG on osteogenic differentiation has been seldom studied. EGCG was hypothesized to function as an enhancer or an inducer. In the present study, the effect of EGCG on the osteogenic differentiation of primary human bone marrow mesenchymal stem cells (hBMSCs), without other additives, was investigated. Three groups of stem cells were analyzed, which included a negative control group (hBMSCs cultured with culture medium only), an experimental group (cells treated with culture medium containing 2.5, 5 and 10 µM EGCG), and a positive control group (cells cultured with osteogenesis-induced culture medium). After 3, 7, 14 and 21 days, cell proliferation, alkaline phosphatase (ALP) activity and the expression of associated osteogenic genes were analyzed. The results revealed that ALP activity and the expression of associated osteogenic genes, with the exception of bone morphogenetic protein 2 (BMP2), were not affected by EGCG treatment alone. These results indicated that EGCG itself had little effect on the osteogenic differentiation of MSCs; however, EGCG was able to enhance osteogenesis in the presence of osteoinductive agents through the upregulation of BMP2 expression. Additionally, EGCG was shown to promote cell growth, demonstrating its safety as a therapeutic agent. Therefore, the present study indicated that treatment with EGCG was dependent on other osteogenic inducers.

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Section: Introductionmentioning

confidence: 99%

Role of (-)-epigallocatechin-3-gallate in the osteogenic differentiation of human bone marrow mesenchymal stem cells: An enhancer or an inducer?

Jin

et al. 2015

Experimental and Therapeutic Medicine

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“…On the contrary, MAR is a more dynamic and objective bone histomorphometric marker that considers the time-course bone remodeling activity, and relies on two very distinct fluorescent colors (red versus green). 27,29 As such, high MAR values (6-8 mm/day) were expected for the autograft.…”

Section: Discussionmentioning

confidence: 95%

“…The distance between mineral apposition layers was measured at the interface between the native bone and the implanted scaffold (INBS), both anteriorly, posteriorly, medially, and laterally with Nikon NIS Elements software tools. 27 The mean distance of the four regions was subject to statistical analysis and the overall mean distance was calculated for each rat. The mineral apposition rate (MAR) at INBS or bone healing/ regeneration was calculated for 8 and 12 weeks by dividing the overall mean distance by the interlabeling period of 32 and 60 days, respectively.…”

Section: Assessing Mineral Apposition Rate By Fluorescence Microscopymentioning

confidence: 99%

Titanium-Enriched Hydroxyapatite–Gelatin Scaffolds with Osteogenically Differentiated Progenitor Cell Aggregates for Calvaria Bone Regeneration

Ferreira

Padilla

Urkasemsin

et al. 2013

Tissue Engineering Part A

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Adequate bony support is the key to re-establish both function and esthetics in the craniofacial region. Autologous bone grafting has been the gold standard for regeneration of problematic large bone defects. However, poor graft availability and donor-site complications have led to alternative bone tissue-engineering approaches combining osteoinductive biomaterials and three-dimensional cell aggregates in scaffolds or constructs. The goal of the present study was to generate novel cell aggregate-loaded macroporous scaffolds combining the osteoinductive properties of titanium dioxide (TiO 2 ) with hydroxyapatite-gelatin nanocomposites (HAP-GEL) for regeneration of craniofacial defects. Here we investigated the in vivo applicability of macroporous (TiO 2 )-enriched HAP-GEL scaffolds with undifferentiated and osteogenically differentiated multipotent adult progenitor cell (MAPC and OD-MAPC, respectively) aggregates for calvaria bone regeneration. The silane-coated HAP-GEL with and without TiO 2 additives were polymerized and molded to produce macroporous scaffolds. Aggregates of the rat MAPC were precultured, loaded into each scaffold, and implanted to rat calvaria criticalsize defects to study bone regeneration. Bone autografts were used as positive controls and a poly(lacticco-glycolic acid) (PLGA) scaffold for comparison purposes. Preimplanted scaffolds and calvaria bone from pig were tested for ultimate compressive strength with an Instron 4411 Ò and for porosity with microcomputerized tomography (mCT). Osteointegration and newly formed bone (NFB) were assessed by mCT and nondecalcified histology, and quantified by calcium fluorescence labeling. Results showed that the macroporous TiO 2 -HAP-GEL scaffold had a comparable strength relative to the natural calvaria bone (13.8 -4.5 MPa and 24.5 -8.3 MPa, respectively). Porosity was 1.52 -0.8 mm and 0.64 -0.4 mm for TiO 2 -HAP-GEL and calvaria bone, respectively. At 8 and 12 weeks postimplantation into rat calvaria defects, greater osteointegration and NFB were significantly present in the TiO 2 -enriched HAP-GEL constructs with OD-MAPCs, compared to the undifferentiated MAPCloaded constructs, cell-free HAP-GEL with and without titanium, and PLGA scaffolds. The tissue-engineered TiO 2 -enriched HAP-GEL constructs with OD-MAPC aggregates present a potential useful therapeutic approach for calvaria bone regeneration.

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“…These findings are confirmed by micro-CT-based analysis and histological evaluation. An earlier report showed that -TCP particle implantation resulted high density areas at the defect margin and between the particles 17) . Moreover, collagen implantation resulted sporadic calcification in the defects 15) .…”

Section: Discussionmentioning

confidence: 93%

Histochemical and Radiological Study of Bone Regeneration by the Combinatorial Use of Tetrapod-Shaped Artificial Bone and Collagen

Uryu

Matsumoto

Namaki

et al. 2015

J. Hard Tissue Biology.

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The purpose of this study was to examine the potential application of a tetrapod-shaped artificial bone, Tetrabone (TB), along with collagen, in bone regeneration. Nine-week-old male Wistar rats (n = 60) were used. An 8 mm-diameter defect was filled with TB, collagen, or a combination mixture of TB and collagen, and left untreated in the control group. The results showed higher volumes of the newly formed bones in the combination group than in the TB group or control group at each time point by micro-CT analysis (P < 0.05). The TB group showed bone formation that extended from the existing bone. In contrast, the collagen group showed disseminated bone formation in the defect. Runx2-and TGF--positive cells were identified at collagen and TB surfaces. The bone formation of the combination group had characteristics of both TB and collagen groups. The combination group showed bone formation from the existing bone edge and in the entire part of the defect. Thus, the combinatorial use of TB and collagen would be an effective strategy for the treatment of bone defect.

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Implantation of green tea catechin α‐tricalcium phosphate combination enhances bone repair in rat skull defects

Abstract: The combination of α-TCP particles and 0.2 mg EGCG stimulates maximum bone regeneration in rat calvarial defects, and this combination would be potentially effective as bone graft material.

Cited by 46 publications

References 54 publications

Role of (-)-epigallocatechin-3-gallate in the osteogenic differentiation of human bone marrow mesenchymal stem cells: An enhancer or an inducer?

Role of (-)-epigallocatechin-3-gallate in the osteogenic differentiation of human bone marrow mesenchymal stem cells: An enhancer or an inducer?

Titanium-Enriched Hydroxyapatite–Gelatin Scaffolds with Osteogenically Differentiated Progenitor Cell Aggregates for Calvaria Bone Regeneration

Histochemical and Radiological Study of Bone Regeneration by the Combinatorial Use of Tetrapod-Shaped Artificial Bone and Collagen

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