Implantation of preadipocyte-loaded hyaluronic acid-based scaffolds into nude mice to evaluate potential for soft tissue engineering

Silk fibroin/hyaluronan scaffolds for human mesenchymal stem cell culture in tissue engineering

“…It has been previously reported that HA can increase the penetration of cells into scaffolds due to increased swelling in biological media [16]. For that reason, we tested the swelling behavior of our scaffolds (Fig.…”

“…In fact, several reports showed that scaffolds modified with HA may enhance cell penetration [16,29,34], through mechanisms unrelated to scaffold microstructure. The work of Holifield et al [19] has cast some light on this issue by showing that CD44 (i.e.…”

“…HA can also interact with cells through receptors on the plasmatic membrane such as CD44. In turn, this receptor activates several biological effects, such as modulation of angiogenic processes [14][15][16][17], induction of proinflammatory metalloprotease expression [18], enhancement of cell motility and invasion [19], and amplification of cell proliferation [20]. Other studies also indicated a key role of the interaction between HA and CD44 at several stages of embryogenesis [21], as well as for the development of adult tissues such as the growth plate or mature bone [22][23][24].…”

Autologous In Vivo Adipose Tissue Engineering in Hyaluronan-Based Gels—A Pilot Study

“…In previous studies, we have demonstrated that open hyaluronan sponges with interconnecting pores of 50 to 340 m had deeper penetration of adipocytes than either collagen sponges or nonwoven hyaluronan meshes [16]. Even larger scaffold pores were shown to allow better cell penetration, although maturation of the progenitor cells was compromised [18].…”

“…In total, 80 to 100 g clean adipose tissue was collected. This fat was cut into small pieces by sterile scissors and afterward digested for 4 h with 0.2% (wt /vol) collagenase Type 1 CLS under conditions described for human preadipocyte isolation [18] (1 mL of collagenase buffered solution/g of fat, shaking at 37°C). Cells were then seeded onto fibronectin-coated culture flasks in proliferation medium (DMEM/F12 supplemented with 1 nM bFGF, 100 U/mL penicillin and 100 g/mL streptomycin) with a seeding density of 3 ϫ 10 4 cells/cm 2 .…”