This paper investigates the breakdown of two microstrip antennas through experiments and particle-in-cell simulations. The breakdown thresholds of a microstrip dipole antenna and a double-layer patch microstrip antenna are investigated experimentally and found to be 15.6 and 30.8 kW, respectively. Ablation is observed on the surface of the antennas, indicating that the local electric field is particularly intense. To further understand the breakdown process, the particle-in-cell and Monte Carlo collision methods are combined to investigate the inception and development of partial discharge on the surface of the antennas. Under the criterion of continuous electron density growth, the breakdown thresholds of the dipole and double-layer patch antennas are estimated to be 19.4 and 52.9 kW in our simulations. The simulation results are in reasonable agreement with the experimental measurements. In the microstrip dipole antenna, the plasma evolution of the partial discharge is initiated near the tip, while the electric field close to the tip is distorted and a positive streamer directed toward the tip is observed. In the double-layer patch microstrip antenna, a more uniform discharge is observed at the edge of the circular patch in the simulations.