“…Hydrocyclones have been employed to provide such a selection pressure; however, their relatively low hydraulic capacity (e.g., 120–3360 m 3 d − 1 ) limits their application in large systems to sidestream return activated sludge (RAS) where only a small portion, e.g., 5–7%, of the RAS flow can be processed for physical selection ( Avila et al., 2021 ; Partin, 2019 ; Regmi et al., 2022 ). This low strength selection pressure plus the high suspended solids content in RAS, e.g., 5000 mg L − 1 , compromised the physical selection efficiency of hydrocyclones for AGS ( Ford et al., 2016 ). As a result, a prolonged startup phase perhaps as long as a year was required to observe significant sludge settleability improvements in full-scale continuous flow reactors in which AGS formation is still a challenge ( Ford et al., 2016 ; Kent et al., 2018 ; Roche et al., 2022 ; Welling et al., 2015 ; Wett et al., 2015 ).…”