Implication of ESR signals from ceruloplasmin (Cu2+) and transferrin (Fe3+) in pleural effusion of lung diseases

Hirota, Yoshio; Haida, Munetaka; Mohtarami, Forogh; Takeda, K.; Iwamoto, Tokuzen; Shioya, Sumie; Tsuji, Chizuko; Hasumi, K; Nakazawa, Hiroe

doi:10.1016/s0928-4680(99)00033-4

Cited by 7 publications

(5 citation statements)

References 25 publications

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“…In the EPR spectra of whole blood occurs also the line for which g = 2.05 (Fig.2). This corresponds to the literature value: g = 2.049 assigned to Cu 2+ ions in ceruloplasmin (Hirota et al, 2000;Pocklington & Foster 1977;Foster, Pocklington, Miller & Mallard, 1973). This is perpendicular hyperfine line of the Cu 2+ signal (Kleinhans, Kline, Dugan & Williams 1983), most intensive component, which comes from the Cu type II (Kouoh Elombo et al, 2000).…”

Section: Resultssupporting

confidence: 84%

“…14 -4.16. Comparison with the literature shows that this signal originates from Fe 3+ in transferrin (g = 4.2) (Hirota, Haida, Mohtarami, Takeda, Iwamoto, Shioya, Tsuji, Hasumi, Nakazawa, 2000;Pocklington & Foster, 1977), which is also confirmed by the very characteristic shape. The first component (counting in the direction of increasing values of magnetic field induction), for which g = 4.30 -4.37, has the lowest amplitude and the narrowest line width.…”

Section: Resultssupporting

confidence: 72%

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EPR Study of Paramagnetic Centers in Human Blood

Kubiak

Krzyminiewski

Dobosz

2013

Current Topics in Biophysics

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Electron paramagnetic resonance investigations of paramagnetic centers in whole human blood were carried out at 170 K using X-band EPR spectrometer. The study included a group of patients and healthy volunteers. The EPR signals from high spin Fe3+ ions in transferrin (g = 4.2) and Cu2+ ions in ceruloplasmin (g = 2.05) are characteristic of each frozen blood sample. An overview of all recorded spectra revealed in several cases additional lines derived from high spin Fe3+ ions in methemoglobin (g = 5.8 - 6), free radicals (g = 2.002 - 2.005) and various low spin ferriheme complexes (g = 2.21 - 2.91). The lines from cytochromes (g = 3.03 and 3.27) were observed only twice. The EPR measurements have not confirmed the correlation between the occurrence of a particular type of low-spin iron complex and a specific disease entity. Moreover, the presence of EPR lines from trivalent iron also did not differentiate patients from healthy volunteers.

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Section: Resultssupporting

confidence: 84%

Section: Resultssupporting

confidence: 72%

EPR Study of Paramagnetic Centers in Human Blood

Kubiak

Krzyminiewski

Dobosz

2013

Current Topics in Biophysics

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“…The g factor, assigned to the line formed by the merger of two components with highest amplitudes, is in the g range of 4.14–4.16. Comparison with the literature shows that this signal originates from Fe 3+ in transferrin ( g = 4.2) (Hirota et al 2000 ; Kubiak et al 2013 ; Pocklington and Foster 1977 ), which is also confirmed by the very characteristic shape. Two non-heme iron atoms are in the high-spin state S = 5/2 and their EPR spectrum is characteristic of the compounds in which iron is present in the rhombic symmetry system (Krzyminiewski et al 2011 ; Pinkowitz and Aisen 1972 ) in an octahedral surrounding (Krzyminiewski et al 2011 ).…”

Section: Resultssupporting

confidence: 64%

“…Two distinct EPR lines were detected in all frozen samples. Firstly, a line corresponding to g = 2.05 which corresponds to the literature value g = 2.049, which is assigned to Cu 2+ ions in ceruloplasmin (Foster et al 1973 ; Hirota et al 2000 ; Pocklington and Foster 1977 ). The characteristic shape of the signal also confirms the origin of the line.…”

Section: Resultsmentioning

confidence: 86%

“…It can also catalyze the reoxidation of Cu + to Cu 2+ . Only Cu 2+ ions can be studied by the method of electron paramagnetic resonance, because Cu + ions are EPR silent (Hirota et al 2000 ). The hCP protein contains six copper ions: three paramagnetic ions (Cu type I) in mononuclear sites and a trinuclear cluster with one paramagnetic (Cu type II) and two antiferromagnetically coupled ions (Cu type III) (Healy and Tipton 2007 ).…”

Section: Introductionmentioning

confidence: 99%

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The influence of radiotherapy on ceruloplasmin and transferrin in whole blood of breast cancer patients

Krzyminiewski

Dobosz

Kubiak

2017

Radiat Environ Biophys

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Ceruloplasmin and transferrin are proteins which play a potential role in the process of breast cancer development. These molecules contain Cu2+ (ceruloplasmin) or Fe3+ ions (transferrin) and thus constitute paramagnetic centers, which can be studied using electron paramagnetic resonance method. The aim of the study was to determine how paramagnetic centers in whole blood of breast cancer patients change under the influence of radiation therapy. Samples of whole blood were taken from 17 women with breast cancer treated with radiotherapy. The measurements were carried out at 170 K using X-band electron paramagnetic resonance (EPR) spectrometer Bruker EMX-10. Two distinct EPR lines, derived from high-spin Fe3+ in transferrin and Cu2+ from ceruloplasmin, were revealed in all frozen samples. The amplitude and integrated intensity of the EPR signal from Cu2+ in ceruloplasmin significantly decreased in all patients after the delivery of the radiation fraction. When comparing the integral intensity of the signal from Fe3+ in transferrin, three different situations were identified which are patient specific: a significant increase, an insignificant change, or a significant decrease after the irradiation. A decreased level of Cu2+ from ceruloplasmin in patients after radiotherapy means a low level of ceruloplasmin in the plasma or an increased content of reduced Cu+ ions. Differences in the integrated intensity of the EPR signal from transferrin translate directly into the amount of bound iron. The observed changes could indicate how well the organism fights against cancer and how easily it adapts to the situation of biochemical stress.

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Proteomics analysis of serous fluids and effusions: Pleural, pericardial, and peritoneal

Tyan

Liao

2007

Proteomics Clinical Apps

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Serous body fluids are normally present in small amounts in the cavities lined by serous membranes, and effusions are the term usually used to represent an excess of these fluids. Thus, any changes in the effusions may accurately reflect various pathological processes. In this review, we will introduce the present status of effusion analysis. Proteomics allows the global study of protein expression and offers a comprehensive view to analyze effusion proteins at a system level. It deals with the study of proteins, their structures, localizations, PTM, functions, and interactions with other proteins. This paper reviews the variety of analytical methods that have been used for the analysis of effusions. The proteins expressed in effusions, which have been identified by proteomics methods are discussed.

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Implication of ESR signals from ceruloplasmin (Cu2+) and transferrin (Fe3+) in pleural effusion of lung diseases

Cited by 7 publications

References 25 publications

EPR Study of Paramagnetic Centers in Human Blood

EPR Study of Paramagnetic Centers in Human Blood

The influence of radiotherapy on ceruloplasmin and transferrin in whole blood of breast cancer patients

Proteomics analysis of serous fluids and effusions: Pleural, pericardial, and peritoneal

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