Implications and challenges of tuberculosis in wildlife ungulates in Portugal: A molecular epidemiology perspective

Cunha, Mónica V.; Matos, Filipa; Canto, Ana; Albuquerque, Teresa; Alberto, João R.; Aranha, José; Vieira-Pinto, Madalena; Botelho, Ana

doi:10.1016/j.rvsc.2011.03.009

Cited by 39 publications

(84 citation statements)

References 26 publications

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“…These studies showed that high levels of discrimination could be achieved by spoligotyping (discriminatory index [D] ϭ 0.89 and 0.87 in Portugal and Spain, respectively), although subsequent studies from Portugal found that discrimination actually increased with MIRU-VNTR typing (12,13).…”

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confidence: 99%

“…However, in some geographical settings, this cost-effective high-throughput technique does not offer adequate discrimination (2,3,4) and is thus complemented with mycobacterial interspersed repetitive-unit-variablenumber tandem-repeat (MIRU-VNTR) typing (5,6). The MIRU-VNTR technique has been evaluated in many countries using different sets of makers (2,4,(7)(8)(9)(10)(11)(12)(13)(14)(15).…”

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confidence: 99%

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Splitting of a Prevalent Mycobacterium bovis Spoligotype by Variable-Number Tandem-Repeat Typing Reveals High Heterogeneity in an Evolving Clonal Group

et al. 2013

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Bovine tuberculosis (Mycobacterium bovis) remains a concern in Spain; although herd prevalence has been considerably reduced since the systematic implementation of the national eradication program in the 1990s, infection still affected 1.33% of herds in 2011 (per the Ministry of the Environment and Rural and Marine Affairs). In consideration of the economic and public health implications of this zoonosis, tracing the source of infection by genotyping has become a key element within the program. The most widely used technique to type M. bovis isolates is direct variable repeat (DVR) spoligotyping (1). However, in some geographical settings, this cost-effective high-throughput technique does not offer adequate discrimination (2, 3, 4) and is thus complemented with mycobacterial interspersed repetitive-unit-variablenumber tandem-repeat (MIRU-VNTR) typing (5, 6). The MIRU-VNTR technique has been evaluated in many countries using different sets of makers (2, 4, 7-15).In recent surveys in the Iberian Peninsula, SB0121 was found to be the most frequent spoligotype of M. bovis (26.3% in Portugal [16] and 27.9% in Spain [17]). These studies showed that high levels of discrimination could be achieved by spoligotyping (discriminatory index [D] ϭ 0.89 and 0.87 in Portugal and Spain, respectively), although subsequent studies from Portugal found that discrimination actually increased with MIRU-VNTR typing (12,13).In this study, we used nine MIRU-VNTR markers to type a panel of randomly selected Spanish M. bovis isolates with the spoligotype SB0121. The allelic diversity and overall discriminatory power of the technique were evaluated, using several different formats. Additionally, MIRU-VNTR was applied to all the infected animals from farms infected with M. bovis SB0121 to evaluate if heterogeneity was underdetected. MATERIALS AND METHODSMycobacterial strains. The study was carried out in M. bovis isolates with spoligotype SB0121 that were sampled from across Spain between 1997 and 2010. To evaluate the discriminatory power of MIRU-VNTR, we used a panel (n ϭ 115) (see Table S1 in the supplemental material) comprising 111 isolates selected randomly from 3,863 M. bovis SB0121 isolates from the Spanish strain collection at the VISAVET Health Surveillance Centre affecting animals plus four additionally selected M. bovis isolates from less frequently affected species. The isolates originated from cattle (Bos taurus) (n ϭ 98), goats (Capra aegagrus hircus) (n ϭ 2), wild boars (Sus scrofa) (n ϭ 7), red deer (Cervus elaphus) (n ϭ 2), fallow deer (Dama dama) (n ϭ 2), badgers (Meles meles) (n ϭ 2 [18]), and domestic pigs (Sus scrofa domestica) (n ϭ 2).To evaluate potential heterogeneity within the farms infected by M. bovis SB0121, we randomly selected 15 farms from 1,342 cattle farms from which isolates were included in the strain collection to design a second panel that comprised all the SB0121 isolates obtained from infected animals from these farms (total n ϭ 176; number of isolates per farm, 2 to 31).

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Splitting of a Prevalent Mycobacterium bovis Spoligotype by Variable-Number Tandem-Repeat Typing Reveals High Heterogeneity in an Evolving Clonal Group

et al. 2013

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“…On the other hand, wild ungulate translocations for hunting purposes occur frequently and may spread M. bovis to areas where it is absent today. Interestingly, M. bovis was isolated from wild boar in Portugal in two areas widely out of the known distribution of the disease (Santos et al, 2009;Cunha et al, 2012), one of which coincides with the release site of red deer originating from a population harbouring the same genotype of M. bovis. This provides circumstantial evidence for the role of translocations on bTB geographical spread.…”

Section: Trendsmentioning

confidence: 99%

Wildlife Tuberculosis: A Systematic Review of the Epidemiology in Iberian Peninsula

Santos¹,

Correia-Neves²,

Almeida³

et al. 2012

Epidemiology Insights

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“…MAC infection in dogs is not frequent (4)(5)(6) and it diagnosis is difficult because of its nonspecific presentation (7). However, a few reports indicated that dogs infected by the MAC present with lymphadenopathy, hematologic abnormalities, and cutaneous granulomas (8)(9)(10).…”

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“…The MAC can cause infection in both humans and animals (7,12). In dogs, MAC infection has been recently described by PCR (5,13).…”

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Serological survey for antibodies against Mycobacterium avium complexin hunting dogs in East-Central Portugal

Coelho¹,

Matos²,

Figueira³

et al. 2016

Turk J Vet Anim Sci

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Implications and challenges of tuberculosis in wildlife ungulates in Portugal: A molecular epidemiology perspective

Cited by 39 publications

References 26 publications

Splitting of a Prevalent Mycobacterium bovis Spoligotype by Variable-Number Tandem-Repeat Typing Reveals High Heterogeneity in an Evolving Clonal Group

Splitting of a Prevalent Mycobacterium bovis Spoligotype by Variable-Number Tandem-Repeat Typing Reveals High Heterogeneity in an Evolving Clonal Group

Wildlife Tuberculosis: A Systematic Review of the Epidemiology in Iberian Peninsula

Serological survey for antibodies against Mycobacterium avium complexin hunting dogs in East-Central Portugal

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