Implications of Aquaglyceroporin 7 in Energy Metabolism

Iena, Francesco Maria; Lebeck, Janne

doi:10.3390/ijms19010154

Cited by 31 publications

(27 citation statements)

References 89 publications

(178 reference statements)

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“…Even though measurements of enzyme activity should have been applied to fully elucidate the lipolytic rate in WAT in this study, the findings in the present study are in line with the notion that even though the overall lipolytic activity is increased in response to obesity, the lipolytic activity per kilogram fat mass is actually reduced in obesity (39). Studies in male rodents have led to the general assumption that the AT AQP7 abundance is increased in states with increased lipolytic rate such as fasting (40). However, in fasted male mice increased abundance of AQP7 protein was only found after 72 h, but not after 24 h of fasting (22).…”

Section: Discussionsupporting

confidence: 83%

Sex-Specific Effect of High-Fat Diet on Glycerol Metabolism in Murine Adipose Tissue and Liver

et al. 2020

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Obesity is associated with increased plasma glycerol levels. The coordinated regulation of glycerol channels in adipose tissue (AQP7) and the liver (AQP9) has been suggested as an important contributor to the pathophysiology of type-2-diabetes mellitus, as it would provide glycerol for hepatic synthesis of glucose and triglycerides. The regulation of AQP7 and AQP9 is influenced by sex. This study investigates the effect of a high-fat diet (HFD) on glycerol metabolism in mice and the influence of sex and GLP-1-receptor agonist treatment. Female and male C57BL/6JRj mice were fed either a control diet or a HFD for 12 or 24 weeks. Liraglutide was administered (1 mg/kg/day) to a subset of female mice. After 12 weeks of HFD, females had gained less weight than males. In adipose tissue, only females demonstrated an increased abundance of AQP7, whereas only males demonstrated a significant increase in glycerol kinase abundance and adipocyte size. 24 weeks of HFD resulted in a more comparable effect on weight gain and adipose tissue in females and males. HFD resulted in marked hepatic steatosis in males only and had no significant effect on the hepatic abundance of AQP9. Liraglutide treatment generally attenuated the effects of HFD on glycerol metabolism. In conclusion, no coordinated upregulation of glycerol channels in adipose tissue and liver was observed in response to HFD. The effect of HFD on glycerol metabolism is sex-specific in mice, and we propose that the increased AQP7 abundance in female adipose tissue could contribute to their less severe response to HFD.

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Section: Discussionsupporting

confidence: 83%

Sex-Specific Effect of High-Fat Diet on Glycerol Metabolism in Murine Adipose Tissue and Liver

et al. 2020

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“…As a pore-forming transmembrane protein, the aquaglyceroporin AQP7 facilitates the transport of glycerol across cell membranes [18]. AQP7 involves in rapid uid movement through the villus epithelium in the small and large intestine [19]. Therefore, FP intervention downregulated Gk to inhibit the synthesis of TG, and upregulated Aqp7 to facilitate the transport of glycerol that would be metabolized in other tissues.…”

Section: Fp Intervention Promoted Lipid Metabolism Of the Colonic Epimentioning

confidence: 99%

“…Caused by the complex interactions between genes and environmental factors, including food components, diet and/or way of life, obesity is one of the most serious public health challenges facing the world [1]. Over 340 million children and adolescents aged [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19] in 2016, as well as an estimated 38.2 million children under the age of 5 years in 2019, were overweight or obese [2]. Developing from an imbalance between energy expenditure and energy intake, obesity is a risk factor for the development of several clinical conditions, such as diabetes, cancers and cardiovascular disease [3].…”

Section: Introductionmentioning

confidence: 99%

Flaxseed Polysaccharide Altering Colonic Gene Expression of Lipid Metabolism and Energy Metabolism in Obese Rat

Lin¹,

Qi²,

Zhang³

et al. 2020

Preprint

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BackgroundObesity is one of the most serious public health challenges. Recently, we found that flaxseed polysaccharide (FP) had an anti-obesity effect through promoting lipid metabolism, inducing satiety and regulating gut microbiota, but how FP promote lipid metabolism through altering the colonic epithelial cells remains to be elucidated. In this study, a transcriptome study was performed to investigate the effect of FP altering the gene expression of colonic epithelial cells in an obese rat model. ResultsThe transcriptome analysis showed that 3,785 genes were differentially expressed after FP intervention in colonic epithelial cells, including 374 down-regulated and 3,411 up-regulated genes. Through KEGG analysis, we found out three classical pathways related to lipid metabolism and energy metabolism, including PPAR signaling pathway, nitrogen metabolism and oxidative phosphorylation (OXPHOS). Moreover, qRT-PCR results showed consistent expression trends of differential genes with transcriptome analysis. ConclusionsThe anti-obesity effect of FP may be achieved by regulating the expression of lipid metabolism- and energy metabolism-related proteins acting on the PPAR (peroxisome proliferator-activated receptor) signaling pathway, nitrogen metabolism and OXPHOS pathway in vivo.

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“…Glycerol is a necessary constituent of triglyceride (TG) backbones, and glycerol uptake together with release across the plasma membrane are two key steps in triglyceride synthesis (lipogenesis) and hydrolysis (lipolysis) in adipose, liver, and other metabolic organs, thus AQPs have emerged as key players in adipose biology and the development of obesity [4]. In this context, AQP7, the first identified AQP in adipose tissue, has been emerging as an important player in whole-body metabolism and the progress of obesity and diabetes [5]. In recent studies, expression of AQP3, AQP9, AQP10, and AQP11 has been detected in cultured adipocytes and adipose tissues [6][7][8].…”

Section: Introductionmentioning

confidence: 99%

AQP3 Facilitates Proliferation and Adipogenic Differentiation of Porcine Intramuscular Adipocytes

Wang

Yang

Yao

et al. 2020

Genes

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The meat quality of animal products is closely related to the intramuscular fat content. Aquaglyceroporin (AQP) defines a class of water/glycerol channels that primarily facilitate the passive transport of glycerol and water across biological membranes. In this study, the AQP3 protein of the AQP family was mainly studied in the adipogenic function of intramuscular adipocytes in pigs. Here, we found that AQP3 was increased at both mRNA and protein levels upon adipogenic stimuli in porcine intramuscular adipocytes in vitro. Western blot results showed knockdown of AQP3 by siRNA significantly suppressed the expression of adipogenic genes (PPARγ, aP2, etc.), repressed Akt phosphorylation, as well as reducing lipid accumulation. Furthermore, deletion of AQP3 by siRNA significantly downregulated expression of cell cycle genes (cyclin D, E), and decreased the number of EdU-positive cells as well as cell viability. Collectively, our data indicate that AQP3 is of great importance in both adipogenic differentiation and proliferation in intramuscular adipocytes, providing a potential target for modulating fat infiltration in skeletal muscles.

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Implications of Aquaglyceroporin 7 in Energy Metabolism

Cited by 31 publications

References 89 publications

Sex-Specific Effect of High-Fat Diet on Glycerol Metabolism in Murine Adipose Tissue and Liver

Sex-Specific Effect of High-Fat Diet on Glycerol Metabolism in Murine Adipose Tissue and Liver

Flaxseed Polysaccharide Altering Colonic Gene Expression of Lipid Metabolism and Energy Metabolism in Obese Rat

AQP3 Facilitates Proliferation and Adipogenic Differentiation of Porcine Intramuscular Adipocytes

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