Importance of chromatographic and spectrophotometric methods in determining authenticity, classification and bioactivity of honey

Güzelmeriç, Etil; Ciftci, Irem; Yuksel, Parla Isil; Yeşilada, Erdem

doi:10.1016/j.lwt.2020.109921

Cited by 25 publications

(23 citation statements)

References 19 publications

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“…Among them, indole-3-carboxylic acid and caffeic acid were 6.36 ± 0.01 and 180.69 ± 0.01 µg/mL, respectively, which was about 28 times the content difference. LODs of 0.029 and 0.134 mg/L, and LOQs of 0.086 and 0.403 mg/L of caffeic acid and cholorogenic acid, which are contained present in high amount in Gynura procumbens, which is lower than the previous studies (caffeic acid-LOD (0.08 µg/mL), LOQ (0.27 ug/mL), cholorogenic acid-LOD (0.79 ug/mL), LOQ (2.62 ug/mL)) [33]. Since lower limits of detection (LOD) and limits of quantitation (LOQ) indicate that the precision of the analytical equipment is better, the LOD and LOQ results of this study are considered to have high precision.…”

Section: Methods Performance Validation and Quantificationcontrasting

confidence: 68%

Potential Antioxidant and Anti-Inflammatory Function of Gynura procumbens Polyphenols Ligand

Kim

Vetrivel

et al. 2021

IJMS

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The antioxidant and anti-inflammatory potentials of polyphenols contained in Gynura procumbens (GP) extract were systematically analyzed. Polyphenols in GP were analyzed for nine peaks using high-performance liquid chromatography (HPLC) combined with mass spectrometry (MS), and quantitatively determined through each standard. A total of nine polyphenolic compounds were identified in the samples and their MS data were tabulated. To determine the potential of bioactive ingredients targeting DPPH and COX-2, we analyzed them by ultrafiltration combined with LC. The results identified the major compounds exhibiting binding affinity for DPPH and COX-2. Caffeic acid, kynurenic acid, and chlorogenic acid showed excellent binding affinity to DPPH and COX-2, suggesting that they can be considered as major active compounds. Additionally, the anti-inflammatory effect of GP was confirmed in vitro. This study will not only be used to provide basic data for the application of GP to the food and pharmaceutical industries, but will also provide information on effective screening methods for other medicinal plants.

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Section: Methods Performance Validation and Quantificationcontrasting

confidence: 68%

Potential Antioxidant and Anti-Inflammatory Function of Gynura procumbens Polyphenols Ligand

Kim

Vetrivel

et al. 2021

IJMS

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“…Thin layer chromatography–direct bioautography (TLC–DB) is a well-known method to detect the antibacterial activity of dissolved samples, but it is less frequently used with honey samples [ 52 ]. Thus, as the first step, we optimized the TLC–DB assay successfully, which allowed us to detect the inhibition zones of honey samples against respiratory tract pathogens as shown for P. aeruginosa in Figure 2 .…”

Section: Resultsmentioning

confidence: 99%

“…Since the latter technique was used only in a few instances with honey, e.g. [ 52 ], our research group had to optimize the process for use with honey samples. Optimizing TLC–DB for two Haemophilus species involved altering conditions such as incubation time, and the composition of agar for growing the bacterium, the necessity of which was also confirmed in our previous study [ 65 ].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Antibacterial and Antibiofilm Activity of Hungarian Honeys against Respiratory Tract Bacteria

Balázs

Nagy-Radványi

Filep

et al. 2021

Foods

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Honey is a rich source of carbohydrates, while minor compounds such as amino acids and polyphenols contribute to its health-promoting effects. Honey is one of the oldest traditional remedies applied for microbial infections, due to its antibacterial, anti-inflammatory, and antioxidant properties. The aim of this study was to investigate the antibacterial and antibiofilm effects of Hungarian black locust, linden, and sunflower honeys against the most common biofilm-forming respiratory tract pathogens Haemophilus spp., Pseudomonas aeruginosa, and Streptococcus pneumoniae. The unifloral character of all three honey types was confirmed by melissopalynological analysis. The antibacterial activity of each honey sample against each bacterium strain was proven with agar well diffusion assay and thin layer chromatography—direct bioautography. Kinetics and mechanisms of antibacterial action were clarified with time-kill assay and membrane degradation study. The anti-biofilm activity was evidenced using crystal violet assay. In each assay, linden honey was the most effective, followed by sunflower and black locust honey. In addition, each honey sample had greater potential to suppress respiratory tract bacteria, compared to major sugar components. In conclusion, honey in general and linden honey in particular, can have a role in the treatment of respiratory tract infections caused by biofilm-forming bacteria.

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“…In addition, high-performance thin-layer chromatographic (HPTLC) analysis of phenolic compounds in pine, chestnut and sunflower types of honey from Turkey showed that each type of honey had a characteristic HPTLC fingerprint. The main compounds in sunflower honey samples were chlorogenic acid, quercetin and caffeic acid; chestnut honey samples were rich in phenolic acids, while quercetin and caffeic acid were characteristic markers for the pine honey samples [ 10 ]. Moreover, the content of these bioactive compounds may vary to a large extent depending upon the geographical origin.…”

Section: Bioactive Compounds In Bee Productsmentioning

confidence: 99%

Bioactives from Bee Products and Accompanying Extracellular Vesicles as Novel Bioactive Components for Wound Healing

Peršurić

Pavelić

2021

Molecules

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In recent years, interest has surged among researchers to determine compounds from bee products such as honey, royal jelly, propolis and bee pollen, which are beneficial to human health. Mass spectrometry techniques have shown that bee products contain a number of proven health-promoting compounds but also revealed rather high diversity in the chemical composition of bee products depending on several factors, such as for example botanical sources and geographical origin. In the present paper, we present recent scientific advances in the field of major bioactive compounds from bee products and corresponding regenerative properties. We also discuss extracellular vesicles from bee products as a potential novel bioactive nutraceutical component. Extracellular vesicles are cell-derived membranous structures that show promising potential in various therapeutic areas. It has been extensively reported that the use of vesicles, which are naturally formed in plant and animal cells, as delivery agents have many advantages. Whether the use of extracellular vesicles from bee products represents a new solution for wound healing remains still to be elucidated. However, promising results in specific applications of the bee products in wound healing and tissue regenerative properties of extracellular vesicles provide a good rationale to further explore this idea.

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Importance of chromatographic and spectrophotometric methods in determining authenticity, classification and bioactivity of honey

Cited by 25 publications

References 19 publications

Potential Antioxidant and Anti-Inflammatory Function of Gynura procumbens Polyphenols Ligand

Potential Antioxidant and Anti-Inflammatory Function of Gynura procumbens Polyphenols Ligand

In Vitro Antibacterial and Antibiofilm Activity of Hungarian Honeys against Respiratory Tract Bacteria

Bioactives from Bee Products and Accompanying Extracellular Vesicles as Novel Bioactive Components for Wound Healing

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