Importance of Citrullination on Hair Protein Molecular Assembly During Trichocytic Differentiation

Kizawa, Kenji; Unno, Masaki; Heizmann, Claus W.; Takahara, Hidenari

doi:10.1007/978-1-4614-8317-5_8

Cited by 5 publications

(2 citation statements)

References 67 publications

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“…Some IFAP have also been shown to be modified by deimination ( Table 2 and Figure 1 c,d). This modification regulates their sensitivity to proteolysis [ 8 , 30 , 31 , 32 , 33 ] and their ability to be cross-linked by transglutaminases [ 34 , 35 , 36 ], which in turn modulates their structural organization following the loss of positive charges [ 13 ]. The first proteins demonstrated to be deiminated (or citrullinated) by PADs, identified by two-dimensional gel electrophoresis and immunodetection using a specific anti-modified citrulline (AMC) antiserum [ 17 ], were abundant proteins.…”

Section: Introductionmentioning

confidence: 99%

Deimination, Intermediate Filaments and Associated Proteins

Briot

Simon

Méchin

2020

IJMS

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Deimination (or citrullination) is a post-translational modification catalyzed by a calcium-dependent enzyme family of five peptidylarginine deiminases (PADs). Deimination is involved in physiological processes (cell differentiation, embryogenesis, innate and adaptive immunity, etc.) and in autoimmune diseases (rheumatoid arthritis, multiple sclerosis and lupus), cancers and neurodegenerative diseases. Intermediate filaments (IF) and associated proteins (IFAP) are major substrates of PADs. Here, we focus on the effects of deimination on the polymerization and solubility properties of IF proteins and on the proteolysis and cross-linking of IFAP, to finally expose some features of interest and some limitations of citrullinomes.

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Section: Introductionmentioning

confidence: 99%

Deimination, Intermediate Filaments and Associated Proteins

Briot

Simon

Méchin

2020

IJMS

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“…Post-translational protein modification within hair epithelial matrix cells is a crucial terminal differentiation process leading to proper hair shaft formation. 1) Hair texture and resilience are largely influenced by physical properties of outermost cuticle layers.…”

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confidence: 99%

Arachidonate 12-Lipoxygenase Inhibitors Promote S100A3 Citrullination in Cultured SW480 Cells and Isolated Hair Follicles

Kizawa

Fujimori

Kawai

2017

Biological & Pharmaceutical Bulletin

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The human hair shaft is covered with multiple scale-like cuticular layers. During the terminal differentiation stage of immature cuticular cells within the hair follicle, cysteine-rich calcium binding S100A3 protein is predominantly translated, and its arginine residues are converted to citrullines by peptidylarginine deiminases (PADI). In this study, we found several naturally occurring compounds (e.g., hinokitiol, escletin, and quercetin) elevate S100A3 citrullination in a human colorectal adenocarcinoma cell line (SW480). Selected compounds similarly promoted cuticular differentiation within isolated human hair follicles. Their promotive activities correlated with the previously reported inhibitory activities of arachidonate 12-lipoxygenase (ALOX12) in vitro. Microarray analysis revealed that ALOX12 inhibitor remarkably up-regulated heparin-binding epidermal growth factor-like growth factor (HBEGF). ALOX12 inhibitor and recombinant HBEGF similarly regulated expression of PADI genes in SW480 cells. In isolated hair follicles, arachidonic acid strongly promoted S100A3 citrullination along with elevation of HBEGF. These results suggest that ALOX12 inhibition efficiently triggers hair cuticle maturation by modulating arachidonate metabolism in concert with HBEGF.Key words S100A3 citrullination; hair cuticle; hinokitiol; arachidonate 12-lipoxygenase inhibitor; heparinbinding epidermal growth factor-like growth factor; human colorectal adenocarcinoma cell line From hair epithelial matrix cells, the cuticle, cortex, and medulla of the hair fiber and the inner root sheathes are formed. This trichogenic epithelial portion, together with the outer root sheath and dermal papilla, comprise an intact hair follicle. Post-translational protein modification within hair epithelial matrix cells is a crucial terminal differentiation process leading to proper hair shaft formation. 1) Hair texture and resilience are largely influenced by physical properties of outermost cuticle layers.2) Furthermore, a number of studies have shown a correlation between various hair shaft disorders and molecular defects.3) S100A3 protein is a cysteinerich Ca 2+

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Ca2+-Binding Proteins of the EF-Hand Superfamily: Diagnostic and Prognostic Biomarkers and Novel Therapeutic Targets

Heizmann

2019

Methods in Molecular Biology

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Importance of Citrullination on Hair Protein Molecular Assembly During Trichocytic Differentiation

Cited by 5 publications

References 67 publications

Deimination, Intermediate Filaments and Associated Proteins

Deimination, Intermediate Filaments and Associated Proteins

Arachidonate 12-Lipoxygenase Inhibitors Promote S100A3 Citrullination in Cultured SW480 Cells and Isolated Hair Follicles

Ca2+-Binding Proteins of the EF-Hand Superfamily: Diagnostic and Prognostic Biomarkers and Novel Therapeutic Targets

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