Importance of phospholipid bilayer integrity in the analysis of protein–lipid interactions

Drücker, Patrick; Gerke, Volker; Galla, Hans‐Joachim

doi:10.1016/j.bbrc.2014.09.079

Cited by 6 publications

(2 citation statements)

References 40 publications

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“…[2,6] Limitations of monolayers not simulating the double leaflet bilayer property of membranes explains the heightened relative popularity of SLBs, which are extended planar lipid bilayers often generated by Langmuir-Blodgett transfer or liposome (lipid vesicle) fusion onto a SiO 2 or modified gold surface. [7] SLBs are undoubtedly suitable for reconstituting certain peripheral and integral membrane proteins (which protrude through one membrane side) for use in surface sensitive measurement approaches including quartz crystal microbalance with dissipation monitoring [8] and total internal reflection fluorescence [6,9] of protein association properties. However, integral membrane proteins take up space on both intra-and extracellular sides of the lipid bilayer sheet to varying degrees, a property which is often better accommodated by tethered lipid bilayers making use of macromolecules such as polymer cushions.…”

Section: Membrane Mimetic Technology: a Brief Historymentioning

confidence: 99%

Polymer Nanodiscs and Their Bioanalytical Potential

Farrelly

Martin

Thang

2021

Chemistry A European J

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Membrane proteins (MPs) play a pivotal role in cellular function and are therefore predominant pharmaceutical targets. Although detailed understanding of MP structure and mechanistic activity is invaluable for rational drug design, challenges are associated with the purification and study of MPs. This review delves into the historical developments that became the prelude to currently available membrane mimetic technologies before shining a spotlight on polymer nanodiscs. These are soluble nanosized particles capable of encompassing MPs embedded in a phospholipid ring. The expanding range of reported amphipathic polymer nanodisc materials is presented and discussed in terms of their tolerance to different solution conditions and their nanodisc properties. Finally, the analytical scope of polymer nanodiscs is considered in both the demonstration of basic nanodisc parameters as well as in the elucidation of structures, lipidprotein interactions, and the functional mechanisms of reconstituted membrane proteins. The final emphasis is given to the unique benefits and applications demonstrated for native nanodiscs accessed through a detergent free process.

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Section: Membrane Mimetic Technology: a Brief Historymentioning

confidence: 99%

Polymer Nanodiscs and Their Bioanalytical Potential

Farrelly

Martin

Thang

2021

Chemistry A European J

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“…SLBs can easily be obtained on a broad range of native sufaces, such as glass, [ 31 ] quartz, [ 31a ] mica, [ 32 ] silica, [ 20a , 32d , 33 ] porous silica nanospheres, [ 34 ] ITO, [ 35 ] Si 3 N 4 , [ 36 ] photo-oxidized polystyrene surfaces, [ 37 ] as well as single crystals of TiO 2 [ 33b , 38 ] and SrTiO 2.…”

Section: Solid Substratesmentioning

confidence: 99%

Photosynthetic Proteins in Supported Lipid Bilayers: Towards a Biokleptic Approach for Energy Capture

Wang

Roth

Han

et al. 2015

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In nature, plants and some bacteria have evolved an ability to convert solar energy into chemical energy usable by the organism. This process involves several proteins and the creation of a chemical gradient across the cell membrane. To transfer this process in a laboratory environment several conditions have to be met: i) proteins need to be reconstituted into a lipid membrane, ii) the proteins need to be orientated and functional, and finally iii) the lipid membrane should be capable of maintaining chemical and electrical gradients. Investigating the processes of photosynthesis and energy generation in vivo is a difficult task due to the complexity of the membrane and its associated proteins. Solid supported lipid bilayers provide a good model system for systematic investigation of the different components involved in photosynthetic pathway.In this review, we describe the progress made to date in the development of supported lipid bilayer systems suitable for the investigation of membrane proteins and in particular for the reconstitution of proteins involved in light capture.

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