Importance of the experimental setup in research on attractiveness of odours in moths: an example with <i><scp>B</scp>usseola fusca</i>

Calatayud, Paul‐André; Ahuya, Peter; Rü, B. Le

doi:10.1111/eea.12201

Cited by 16 publications

(10 citation statements)

References 33 publications

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“…Dean & Moraes () concluded from their study that the different rates of volatile emission by Bt and non‐Bt plants could be attributed to the differences in feeding behaviour of Helicoverpa zea (Boddie) larvae on the two treatments, and not due to genetic differences between plants. Other studies have also shown that B. fusca females rely more on physical stimuli than volatile stimuli when selecting a host plant (Calatayud et al, , ), displaying the same orientation towards host and non‐host plants in wind tunnel tests. Busseola fusca females will also oviposit on artificial stems (with no chemostimuli) in no‐choice tests (Khan & Saxena, ), suggesting that plant‐chemical stimuli are not essential in the process of selecting a host plant for oviposition.…”

Section: Discussionmentioning

confidence: 99%

Preference of Bt‐resistant and susceptible Busseola fusca moths and larvae for Bt and non‐Bt maize

Visser

Plessis

Erasmus

et al. 2019

Entomologia Exp Applicata

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The sustainability of genetically engineered insecticidal Bacillus thuringiensis Berliner (Bt) maize, Zea mays L. (Poaceae), is threatened by the evolution of resistance by target pest species. Several Lepidoptera species have evolved resistance to Cry proteins expressed by Bt maize over the last decade, including the African maize stem borer, Busseola fusca (Fuller) (Lepidoptera: Noctuidae). The insect resistance management (IRM) strategy (i.e., the high‐dose/refuge strategy) deployed to delay resistance evolution is grounded on certain assumptions about the biology and ecology of a pest species, for example, the interactions between the insect pest and crop plants. Should these assumptions be violated, the evolution of resistance within pest populations will be rapid. This study evaluated the assumption that B. fusca adults and larvae select and colonize maize plants at random, and do not show any preference for either Bt or non‐Bt maize. Gravid female B. fusca moths of a resistant and susceptible population were subjected to two‐choice oviposition preference tests using stems of Bt and non‐Bt maize plants. Both the number of egg batches as well as the total number of eggs laid on each stem were recorded. The feeding preference of Bt‐resistant and susceptible neonate B. fusca larvae were evaluated in choice test bioassays with whorl leaf samples of specific maize cultivars. Although no differential oviposition preference was observed for either resistant or susceptible female moths, leaf damage ratings indicated that neonate larvae were able to detect Bt toxins and that they displayed feeding avoidance behaviour on Bt maize leaf samples.

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Section: Discussionmentioning

confidence: 99%

Preference of Bt‐resistant and susceptible Busseola fusca moths and larvae for Bt and non‐Bt maize

Visser

Plessis

Erasmus

et al. 2019

Entomologia Exp Applicata

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“…The behaviour of 3 rd instar larvae from CCc, VVv, CVv, VVc and CVc ( Fig 1 ; the last letter is in lower case because the insects were reared only up to the 3 rd instar on the control diet [c] or diet enriched with vanillin [v]) were analyzed after 5h of starvation. A Y-tube olfactometer, described by Ngi-Song et al [ 27 ], which has been shown to be useful for demonstrating differences in attractiveness to odours in moths [ 28 ], was used (length of stem: 18 cm; length of each arm: 34 cm; diameter: 4 cm). The closed ends of each chamber were connected with tubing to each arm of the Y-tube.…”

Section: Methodsmentioning

confidence: 99%

Influence of Dietary Experience on the Induction of Preference of Adult Moths and Larvae for a New Olfactory Cue

et al. 2015

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In Lepidoptera, host plant selection is first conditioned by oviposition site preference of adult females followed by feeding site preference of larvae. Dietary experience to plant volatile cues can induce larval and adult host plant preference. We investigated how the parent’s and self-experience induce host preference in adult females and larvae of three lepidopteran stem borer species with different host plant ranges, namely the polyphagous Sesamia nonagrioides, the oligophagous Busseola fusca and the monophagous Busseola nairobica, and whether this induction can be linked to a neurophysiological phenotypic plasticity. The three species were conditioned to artificial diet enriched with vanillin from the neonate larvae to the adult stage during two generations. Thereafter, two-choice tests on both larvae and adults using a Y-tube olfactometer and electrophysiological (electroantennography [EAG] recordings) experiments on adults were carried out. In the polyphagous species, the induction of preference for a new olfactory cue (vanillin) by females and 3rd instar larvae was determined by parents’ and self-experiences, without any modification of the sensitivity of the females antennae. No preference induction was found in the oligophagous and monophagous species. Our results suggest that lepidopteran stem borers may acquire preferences for new olfactory cues from the larval to the adult stage as described by Hopkins’ host selection principle (HHSP), neo-Hopkins’ principle, and the concept of ‘chemical legacy.’

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“…Responses of C. partellus to plant produced volatiles and authentic standards were tested in a two-arm glass olfactometer as described in Calatayud et al (2014). The Y-tube olfactometer had 2.4 cm internal diameter, 3.2 cm external diameter, 20 cm long arm length and 17 cm short arms' length, it had a 75°angle at the Y-junction.…”

Section: The Y-tube Ofactometer Bioassay (Two-choice Test)mentioning

confidence: 99%

Responses of stemborer Chilo partellus to volatiles emitted by maize landraces exposed to signal grass (Brachiaria brizantha)

Magara

Mutyambai

Charles

et al. 2020

Journal of Plant Interactions

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This study sought to evaluate the oviposition responses of Chilo partellus (Lepidoptera: Crambidae) on maize plants exposed to Brachiaria brizantha Stapf following oviposition by C. partellus and nonexposed maize. Two Kenyan maize landraces (Jowi and Nyamula), Latin America landrace (Cuba 91) and WH505 (hybrid variety) (control) were studied. The result demonstrated that C. partellus deposited a significantly lower number of eggs and egg batches on Nyamula, Jowi and Cuba 91 maize plants exposed to B. brizantha with C. partellus eggs compared to nonexposed ones and the exposed hybrid variety. This was because they emitted electrophysiologically active compounds such as (E)-4, 8-dimethyl-1, 3, 7-nonatriene, decanal, (E)-caryophyllene, linalool, linalool (plus nananal), E-β-fernesene, methyl salicylate and (3E, 7E)-4, 8, 12-trimethyl-1, 3, 7, 11-tri-decatetraene that deterred C. partellus from ovipositing more eggs on these plants. Therefore, herbivore-induced plant volatiles (HIPVs) of B. brizantha can be employed to protect the maize crop against C. partellus.

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Importance of the experimental setup in research on attractiveness of odours in moths: an example with Busseola fusca

Cited by 16 publications

References 33 publications

Preference of Bt‐resistant and susceptible Busseola fusca moths and larvae for Bt and non‐Bt maize

Preference of Bt‐resistant and susceptible Busseola fusca moths and larvae for Bt and non‐Bt maize

Influence of Dietary Experience on the Induction of Preference of Adult Moths and Larvae for a New Olfactory Cue

Responses of stemborer Chilo partellus to volatiles emitted by maize landraces exposed to signal grass (Brachiaria brizantha)

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