Importance of the Spreading Solvent Evaporation Time in Langmuir Monolayers

Orbulescu, Jhony; Leblanc, Roger M.

doi:10.1021/jp9012587

Cited by 15 publications

(17 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For adsorption experiments to different lipid monolayers, the lipids were spread at the airwater interface to reach the desired final surface pressure. Thirty minutes were required for solvent evaporation and film stabilization [17]. Then bacillomycin D was injected at different concentrations into the subphase.…”

Section: Adsorption Experiments At Constant Surface Areamentioning

confidence: 99%

Conformational analyses of bacillomycin D, a natural antimicrobial lipopeptide, alone or in interaction with lipid monolayers at the air–water interface

Nasir

Besson

2012

Journal of Colloid and Interface Science

View full text Add to dashboard Cite

Section: Adsorption Experiments At Constant Surface Areamentioning

confidence: 99%

Conformational analyses of bacillomycin D, a natural antimicrobial lipopeptide, alone or in interaction with lipid monolayers at the air–water interface

Nasir

Besson

2012

Journal of Colloid and Interface Science

View full text Add to dashboard Cite

“…Thirty minutes was required for solvent evaporation and film stabilization. 15 Mycosubtilin was then injected at a final concentration of 0.545 μM into the subphase. The lipopeptide adsorption at the airÀwater interface, measured by tensiometry, was followed as an increase in surface pressure.…”

Section: ' Materials and Methodsmentioning

confidence: 99%

“…Lipids (in hexane–ethanol at 0.545 mM) were spread at the air–water interface to reach the desired final surface pressure. Thirty minutes was required for solvent evaporation and film stabilization . Mycosubtilin was then injected at a final concentration of 0.545 μM into the subphase.…”

Section: Methodsmentioning

confidence: 99%

Specific Interactions of Mycosubtilin with Cholesterol-Containing Artificial Membranes

Nasir

Besson

2011

Langmuir

View full text Add to dashboard Cite

Mycosubtilin is a natural antimicrobial lipopeptide produced by Bacillus subtilis strains. It is characterized by its hemolytic and strong antifungal activities. Mycosubtilin interacts with the plasma membranes of sensitive cells. However, the molecular mechanisms of its biological activities have not been completely elucidated. Our purpose was therefore to analyze the interactions of mycosubtilin with biological membranes by using biomimetic membranes such as Langmuir monolayers and multilayers. Structural changes of mycosubtilin, involving its peptide backbone and the side chain of its tyrosyl residue, were observed when the lipopeptide was interacting with cholesterol-containing multilayers. The interactions of mycosubtilin with monolayers constituted by pure lipids and by phosholipid/cholesterol or phospholipid/sphingomyelin/cholesterol were also examined. An original behavior of mycosubtilin toward cholesterol-containing monolayers was found. However, this original behavior was lost when mycosubtilin was interacting with pure cholesterylacetate monolayers. This suggests the involvement of the alcohol group of cholesterol in mycosubtilin-cholesterol interactions within membranes. Moreover, mycosubtilin induced changes in the organization and morphology of cholesterol-containing monolayers, and large condensed domains with different levels of condensation appeared only in the case of DPPC/sphingomyelin/cholesterol monolayer.

show abstract

“…Maximum surface pressure did not exceed 0.2 mN/m upon compression during the three cycles, indicating that the spreading solvent is not surface active. As mentioned in section 2.3.9.1 in Chapter 2, spreading solvents such as chloroform, toluene, and benzene have been previously shown to not significantly surface active when the maximum changes in surface pressure upon the compression 105. 4.3.2.…”

mentioning

confidence: 97%

“…-9. Maximum surface pressure did not exceed 0.4 mN/m upon compression during the three cycles, indicating that the spreading solvent is not surface active.Orbulescu et al105 have shown that spreading solvents such as chloroform, toluene, and benzene were not significantly surface active, when the maximum changes in surface pressure upon the compression of the pure solvent spread on the water subphase at 20 ± 0.5°C were less than 0.5 mN/m. Our data were in good agreement withOrbulescu et al, where maximum changes in surface pressure upon the compression of the spreading solvent (n-hexane:methanol 95:5 v/v) on the air-liquid interface were less than 0.4 mN/m.To ensure that the spreading solvent n-hexane:methanol (95:5 v/v) has the ability to efficiently spread DPPC at the air-liquid interface, γ min and γ max were measured during three compression-expansion cycles.Table 2-10 summarizes the surface properties of the air-liquid interface before and after the addition of DPPC.…”

mentioning

confidence: 99%

Development of a physiologically-relevant in vitro system to study exhaled bioaerosols

Hamed¹

View full text Add to dashboard Cite

Airborne infectious diseases remain a major global health threat. The primary vector for their transmission is coarse and fine droplets, known as bioaerosols, exhaled from infected individuals during natural respiratory maneuvers, such as breathing, coughing and sneezing. Unfortunately, our current knowledge of the mechanisms by which these exhaled bioaerosols are formed in the lungs is in its infancy. In particular, progress in this field has been hindered by the complex structure of the respiratory fluid and the resulting lack of understanding of the biophysical properties of the fluid. 2011 All Rights Reserved Graduate College ii To my beloved parents, Azzam Hamed and Bahia Nazer, and my brothers, Raed, Rami and Fadi for their continuous support and patience iii ACKNOWLEDGMENTS I would like to thank my advisor Dr. Jennifer Fiegel for her unconditional support and help from the initial to the final level. This work would not have been possible without her. I have gained tremendous knowledge and experience from interacting with her and being part of her research group. I also would like to thank my committee members, Drs. ) for their help, fun time and scientific discussions. I have learned a lot from the intellectual exchanges and laboratory meetings we had, in addition to the interesting conversations about life and science. I am also greatly thankful for the undergraduate students, Laura Northrup, Alexander Benson and Kyle Merrill, who helped me in this work and gave me the opportunity to guide them. In addition, I would like to thank my colleague Mohammed Saleh who helped me in analyzing the results of the bioaerosol study. His effort is appreciated. I express my love and heartfelt gratitude to my parents, despite being thousands of miles away; their love, understanding, patience and support were essential throughout this challenging process. I am heartily thankful to my brothers Raed, Rami and Fadi who supported me, believed in me and provided me with strength throughout these years. Finally, I would like to show my gratitude to all my friends with whom I shared an enjoyable time during these years. iv ABSTRACT Airborne infectious diseases remain a major global health threat. The primary vector for their transmission is coarse and fine droplets, known as bioaerosols, exhaled from infected individuals during natural respiratory maneuvers, such as breathing, coughing and sneezing. Unfortunately, our current knowledge of the mechanisms bywhich these exhaled bioaerosols are formed in the lungs is in its infancy. In particular, progress in this field has been hindered by the complex structure of the respiratory fluid and the resulting lack of understanding of the biophysical properties of the fluid.

show abstract

Importance of the Spreading Solvent Evaporation Time in Langmuir Monolayers

Abstract: Supporting InformationAll UV measurements were performed on a Hewlett-Packard 8452A diode array spectrophotometer fitted for air-water interface measurements. A detailed description of the experimental setup was published elsewhere 1 .

Cited by 15 publications

References 10 publications

Conformational analyses of bacillomycin D, a natural antimicrobial lipopeptide, alone or in interaction with lipid monolayers at the air–water interface

Conformational analyses of bacillomycin D, a natural antimicrobial lipopeptide, alone or in interaction with lipid monolayers at the air–water interface

Specific Interactions of Mycosubtilin with Cholesterol-Containing Artificial Membranes

Development of a physiologically-relevant in vitro system to study exhaled bioaerosols

Contact Info

Product

Resources

About