Important parameters affecting quality of vitrified donor oocytes

Teplá, O; Topurko, Zinovij; Mašata, Jaromír; Jirsová, S; Frolikova, Michaela; Komrsková, Kateřina; Minks, Adela; Tuřánek, Jaroslav; Lynnyk, Anna; Kratochvílová, Irena

doi:10.1016/j.cryobiol.2021.03.001

Cited by 3 publications

(1 citation statement)

References 46 publications

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“…The embryos were frozen on day 2 with at least 4 blastomeres, 1 day before transfer they warmed, and the 3-day-old embryos with at least 8 blastomeres were transferred. Cryopreservation and warming were performed using media and Kitazato protocol (Cryotop ® Kitazato, Japan) (Teplá et al, 2021).…”

Section: Assisted Reproductive Proceduresmentioning

confidence: 99%

Free centrifuge sorting method for high‐count sperm preparation improves biological characteristics of human spermatozoa and clinical outcome: A sibling oocytes study

et al. 2022

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Sperm processing for assisted reproductive technologies (ART) aims to separate immotile and debris from the motile spermatozoa in the semen. The purpose of this study was to assess the effect of free centrifuge sorting (FCS) approach based on a combination of rheotaxis and swim-up on sperm biological characteristics and ICSI clinical outcomes. Each semen sample was splitted into two equal parts for 67 ICSI cycles with donation oocytes. Parts were processed with the Direct Swim Up (DSU) (control) and with the FCS method (experimental). Sperm quality was assessed in terms of motility, fine morphology, mitochondrial membrane potential, lipid peroxidation and sperm DNA fragmentation. Also Following ICSI, the clinical outcomes were compared between the groups. Sperm progressive motility (93.5 ± 4.1% vs. 78.6 ± 8.2%; p < 0.001), the fraction of Class I (good) morphology (30.2 ± 9.4% vs. 23.7 ± 8.5%; p < 0.0001) and the rate of mitochondrial membrane potential (77.4 ± 7.2% vs. 66.9 ± 5.7%; p < 0.0001) were significantly higher in the FCS compared to DSU groups. The level of lipid peroxidation (0.5 ± 0.05% vs. 0.6 ± 0.06%; p < 0.0001) and concentration of DNA fragmentation (DF) (7.4 ± 1.6% vs. 15.4 ± 2.6%; p < 0.0001) were lower in sperm from the FCS group compared to DSU group. There were higher rates of high-quality embryo formation (p < 0.001), implantation and clinical pregnancy rates (p = 0.03) in the FCS group compared to the control group. The processing of seminal samples using FCS collected spermatozoa with better biological quality and resulted in higher reproductive outcomes in ICSI cycles.

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Section: Assisted Reproductive Proceduresmentioning

confidence: 99%

Free centrifuge sorting method for high‐count sperm preparation improves biological characteristics of human spermatozoa and clinical outcome: A sibling oocytes study

et al. 2022

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A comparison of the morphokinetic profiles of embryos developed from vitrified versus fresh oocytes

Montgomery

Montgomery²,

Campbell³

et al. 2023

Reproductive BioMedicine Online

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Timing of ICSI with Respect to Meiotic Spindle Status

Teplá

Topurko

Jirsová

et al. 2022

IJMS

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The aim of this study was to evaluate the efficiency of using meiotic spindle (MS) visibility and relative position to the polar body (PB) as indicators of oocyte maturation in order to optimize intracytoplasmic sperm injection (ICSI) timing. This was a cohort study of patients younger than 40 years with planned ICSI, the timing of which was determined by MS status, compared with those without MS evaluation. The angle between PB and MS and MS visibility were evaluated by optical microscope with polarizing filter. Oocytes with MS evaluation were fertilized according to MS status either 5–6 h after ovum pick-up (OPU) or 7–8 h after OPU. Oocytes without MS evaluation were all fertilized 5–6 h after OPU. For patients over 35 years visualization of MS influenced pregnancy rate (PR): 182 patients with MS visualization had 32% PR (58/182); while 195 patients without MS visualization had 24% PR (47/195). For patients under 35 years, visualization of MS did not influence PR: 140 patients with MS visualization had 41% PR (58/140), while 162 patients without MS visualization had 41% PR (66/162). Visualization of MS therefore appears to be a useful parameter for assessment of oocyte maturity and ICSI timing for patients older than 35.

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Important parameters affecting quality of vitrified donor oocytes

Cited by 3 publications

References 46 publications

Free centrifuge sorting method for high‐count sperm preparation improves biological characteristics of human spermatozoa and clinical outcome: A sibling oocytes study

Free centrifuge sorting method for high‐count sperm preparation improves biological characteristics of human spermatozoa and clinical outcome: A sibling oocytes study

A comparison of the morphokinetic profiles of embryos developed from vitrified versus fresh oocytes

Timing of ICSI with Respect to Meiotic Spindle Status

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