Improved anther culture of maize (Zea mays)

Ting, Y. C.; Yu, M. J.; Zheng, Wan-Zhen

doi:10.1016/0304-4211(81)90003-1

Cited by 40 publications

(17 citation statements)

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“…Green and Philips (1975) have first reported regeneration from immature embryos of maize. Since then, maize regeneration has been reported from immature embryos (Duncan et al 1985;Bohorova et al 1995;Ishida et al 1996;Aguado-Santacruz et al 2007), mature embryos (Huang and Wei 2004;Al-Abed et al 2006), nodal regions (Vladimir et al 2006), leaf tissues (Conger et al 1987;Ahmadabadi et al 2007), anthers (Ting et al 1981; Barloy and Beckert 1993), tassel and ear meristems (Pareddy and Petolino 1990), protoplast (Morocz et al 1990), and shoot meristems (Sairam et al 2003). However, immature zygotic embryos are predominantly used for establishing regeneration-competent cells or callus cultures for genetic transformation (Ahmadabadi et al 2007).…”

Section: Introductionmentioning

confidence: 95%

Callus induction and whole plant regeneration in elite Indian maize (Zea mays L.) inbreds

Rakshit

Rashid

Sekhar

et al. 2009

Plant Cell Tiss Organ Cult

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Callus induction and regeneration ability of five elite maize inbred lines, CM 111, CM 117, CM 124, CM 125 and CM 300 were investigated using 14-day-old immature embryos as explants. Genotype, medium, source of auxin and their concentrations influenced induction of callus. Explants grown on Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid at 1 mg l -1 showed the highest frequency of callusing. Among all the media tested, explants grown on N6 medium gave the highest frequency of organogenic callus. Moreover, N6 supplemented with Dicamba promoted higher callus response in terms of both frequency of induction as well as quality, compared to N6 medium with 2,4-D. N6 supplemented with 2 mg l -1 Dicamba induced the highest frequency of organogenic callus. Among the five genotypes tested, CM 124, CM 125, and CM 300 gave the best callus. Explants of both CM 124 and CM 300 incubated on MS medium supplemented with 1 mg l -1 benzyladenine and 0.5 mg l -1 indole acetic acid promoted the highest frequency of shoot induction. Though CM 124 induced higher percentage of shoot formation than CM 300, the mean number of developed shoots per explant was higher for CM 300. The highest frequency of root formation was observed when shoots were grown on MS medium supplemented with 2 mg l -1 naphathalene acetic acid. Percentage of regenerated plants ranged from 54 to 66.

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Section: Introductionmentioning

confidence: 95%

Callus induction and whole plant regeneration in elite Indian maize (Zea mays L.) inbreds

Rakshit

Rashid

Sekhar

et al. 2009

Plant Cell Tiss Organ Cult

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“…The remaining successful species are tropical and subtropical crops and belong to the Panicoideae. Most studies on this subfamily have concerned Zea mays [1,8,15,19] with more limited reports on Coix lachryma-/obi [20], eennisetum [3,15], Saccharum [4,7,12], Setaria italica [2] and Sorghum bicolor [10]. However, in most of these studies, yields of microspore derivatives were very low and it has not been possible to reach definitive conclusions about the relative importance of most components of the cutture process.…”

Section: Introductionmentioning

confidence: 99%

Anther culture of Sorghum bicolor (L.) Moench

Rose

Dunwell

Sunderland

1986

Plant Cell Tiss Organ Cult

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Anther culture of Sorghum bicolor (L.) Moench I. Effect of panicle pretreatment, anther incubation temperature and 2,4-D concentration 15 Abstract. In a study of anther culture in Sorghum bicolor it was found that culture incubation temperature had a significant effect on yields of microspore-derived callus. A temperature of 33°C was superior to 25 °, 28 ° or 37 ° C. With fresh, unpretreated material, callus was produced only from anthers incubated at 33 ° C.Panicle pretreatment at 7 ° , 14 ° , 20 ° , 25 ° or 28 ° C either had no effect or significantly decreased callus yield.Comparison of culture media containing a range of concentrations of 2,4-dichlorophenoxyacetic acid (0-3.0 mg 1-1) showed that callus yield increased significantly with concentration up to 2.5 mg1-1 and then declined slightly. Although 1174 calluses were produced, subsequent differentiation was poor and only four albino plants were regenerated.

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“…Plant regeneration has also been reported from calli initiated from different explants, namely, anthers [2][3][4], glumes [5], immature inflorescence [6], immature tassels [7][8][9], leaf segments [10][11][12], protoplasts [13], seedling segments [14][15][16], shoot tips [17][18][19], shoot apical meristem [20], mature embryos [15,21,22], and immature embryos [23][24][25][26][27][28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Coleoptilar node - A season-independent explant source for in vitro culture in maize (Zea mays L.)

2018

J App Biol Biotech

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In vitro plant regeneration is preliminary requisite in the process of obtaining transgenic plants. Somatic embryogenesis is the most frequently used method of in vitro plant regeneration in maize. In this study, a reliable and reproducible maize regeneration method has been standardized using mature embryos. Inspite, the immature embryos are most suitable explants for somatic embryogenesis, obtaining these explants continuously is a laborious and tedious process, especially in summer. Hence, alternate explants of maize for in vitro regeneration have been chosen from different parts of embryonic axis. This study provides an evaluation of inbred lines and effect of hormonal combinations on total and embryogenic callus induction, their growth rate, and plantlet regeneration. Coleoptilar nodal explants from four inbred lines were cultured on different concentrations and combinations of 2,4-dichloro phenoxyacetic acid (2,4-D) along with kinetin (Kn). Explants grown on Murashige and Skoog medium supplemented with 2,4-D and Kn at a concentration of 5 and 1 mg/L, respectively, were found to be the best one for yielding a high frequency of embryogenic callus. Inbred line, NM81A was found to be the best one for in vitro culture among the four lines used. Combination of 6-benzylaminopurine and Kn at a concentration of 1 mg/L each promoted the highest frequency of shoot induction.

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Improved anther culture of maize (Zea mays)

Cited by 40 publications

References 1 publication

Callus induction and whole plant regeneration in elite Indian maize (Zea mays L.) inbreds

Callus induction and whole plant regeneration in elite Indian maize (Zea mays L.) inbreds

Anther culture of Sorghum bicolor (L.) Moench

Coleoptilar node - A season-independent explant source for in vitro culture in maize (Zea mays L.)

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