Improved antibacterial function of Rifampicin-loaded solid lipid nanoparticles on Brucella abortus

Abstract

The objective of this study was to assess the antibacterial activity of Rifampicin-loaded solid lipid nanoparticles on Brucella abortus 544. Rifampicin-loaded solid lipid nanoparticles were prepared by a modified microemulsion/sonication method and characterized. The results showed the average size about 319.7 nm, PI about 0.20 and zeta potential about 18.4 mv, encapsulation efficacy and drugloading were equal to 95.78 and 34.2%, respectively, with a spherical shape. Drug release lasted for 5 days. The antibacterial activity was statistically significant with p < .05 in bacterial and cell culture media compared to free Rifampicin. It can be concluded that solid lipid nanoparticles can be considered as a promising delivery system for improving the antibacterial activity of Rifampicin against Brucella abortus.

Formulation and pharmacokinetic evaluation of rifampicin solid lipid nanoparticles

“…The endothermic peak of RIF was shifted to a lower temperature in the physical mixture thermogram because the melting lipids tend to solubilize RIF. This result was previously observed by Ghaderkhani et al after the disappearance of the drug melting peak in the physical mixture thermogram of RIF and Stearic acid for SLNs preparation [23]. The thermogram of RIF loaded SLNs (F4) showed both endothermic and exothermic peak.…”

Solid Lipid Nanoparticles for Drug Delivery: Pharmacological and Biopharmaceutical Aspects

“…• Promoting accumulation in target cells by means of active targeting (Maretti et al, 2017;Costa et al, 2018;Vieira et al, 2018;Hosseini et al, 2019;Banerjee et al, 2020). • Increasing inhibitory effect (i.e., decreasing MIC) over bacterial strains (Severino et al, 2015;Pignatello et al, 2017;Ghaderkhani et al, 2019;Rodenak-Kladniew et al, 2019).…”

Olive Leaves (<i>Olea europaea</i> L) Extract Loaded Lipid Nanoparticles: Optimization of Processing Parameters by Box-Behnken Statistical Design, <i>in-vitro</i> Characterization, and Evaluation of Anti-oxidant and Anti-microbial Activity

“…The value of the zone of inhibition of OPL-SLNs against Pseudomonas aeruginosa and Staphylococcus aureus was observed 14.75±2.25 mm and 16.30±2.1 mm in 24 h respectively. Moreover, study findings indicated that all formulation containing OLP extract exhibited better anti-microbial efficiency towards Gram-positive Staphylococcus aureus as compared to Gram-negative Pseudomonas aeruginosa 40,47) . To the best of our knowledge, and the previous report showed that the major constituents for antimicrobial activity present in the olive extract are Cyclotrisiloxane hexamethyl (36.98％) , Cyclotetrasiloxane octamethyl (15.18％) , and Cyclopentasiloxane decamethyl (14.59％) 2) .…”

“…The antimicrobial study of OLP-loaded SLNs (optimized batch F9) was conducted by utilizing the agar well diffusion method. Bacterial strains like Staphylococcus aureus (Gram s positive) and Pseudomonas aeruginoza (Gram s negative) were used for the evaluation 40) . Nutrient agar plates were prepared under sterile conditions and bacterial suspension (100 µL, concentration around 10 6 CFU/mL) of each bacterium was placed individually on each sterile nutrient agar plate with the help of sterile swabs incubated at 37±0.5℃ for 1 h. Four wells around 8 mm diameters each were produced on each plate with the help of a sterile cork borer.…”