Improved Antioxidant and Anti-inflammatory Potential in Mice Consuming Sour Cherry Juice (Prunus Cerasus cv. Maraska)

Abstract

The present investigation tested the in vivo antioxidant efficacy (superoxide dismutase, SOD; catalase, CAT; glutathione peroxidase; Gpx), lipid peroxidation (LPO) and anti-inflammatory properties (cyclooxygenase-2; COX-2) of sour cherry juices obtained from an autochthonous cultivar (Prunus cerasus cv. Maraska) that is grown in coastal parts of Croatia. Antioxidant potential was tested in mouse tissue (blood, liver, and brain), LPO (liver, brain) and anti-inflammatory properties in glycogen elicited macrophages. Additionally, the concentration of cyanidin-3-glucoside, cyanidin-3-rutinoside, pelargonidin-3-glucoside, pelargonidin-3-rutinoside and total anthocyanins present in Prunus cerasus cv. Maraska cherry juice was determined. Mice were randomly divided into a control group (fed with commercial food pellets) and 2 experimental groups (fed with commercial food pellets with 10% or 50% of cherry juice added). Among the anthocyanins, the cyanidin-3-glucoside was present in the highest concentration. These results show antioxidant action of cherry juice through increased SOD (liver, blood) and Gpx (liver) activity and decreased LPO concentration. The study highlights cherry juice as a potent COX-2 inhibitor and antioxidant in the liver and blood of mice, but not in the brain. Thus, according to our study, Prunus cerasus cv. Maraska cherry juice might potentially be used as an antioxidant and anti-inflammatory product with beneficial health-promoting properties.

Potential application of pulsed electric fields to improve the recovery of bioactive compounds from sour cherries and their by-products

“…Sour cherries (Prunus cerasus L) (also called tart cherries) are an important source of anthocyanins and other phytochemicals that possess many potential biological properties (Damar et al, 2012;Šarić et al, 2009). Studies have demonstrated that sour cherries exhibit anticarcinogenic effect in various colon cancer models (Ferreti et al, 2010).…”

Sesamol suppresses the inflammatory response by inhibiting NF-κB/MAPK activation and upregulating AMP kinase signaling in RAW 264.7 macrophages

“…A recent study discovered that some natural products could ameliorate antioxidant responses and the inflammatory response in LPS-stimulated macrophages [9,15,16]. Sesamun indicum, Linn.…”

Antihyperlipidemic Effects of Sour Cherries Characterized by Different In Vitro Antioxidant Power and Polyphenolic Composition

“…The red skin and flesh of this fruit contain anthocyanins which have strong antioxidant and anti-inflammatory activities. Cherries were found to have anti-inflammatory effects, increase the antioxidant status and inhibit intestinal tumor development in ApcMin mice and reduce proliferation of human colon cancer cells [11][12][13]. In hyperlipidemic rats, the cherryenriched diet was associated with reduced fasting blood glucose, hyperlipidemia, hyperinsulinemia, and reduced fatty liver after 90 days of treatment, and also reduced several phenotypes of metabolic syndrome and both systemic and local inflammation (e.g., it decreased the retroperitoneal interleukin-6 and tumor necrosis factor-alpha activities) [14,15].…”