Improved Biobutanol Production in 2-L Simultaneous Saccharification and Fermentation with Delayed Yeast Extract Feeding and in-situ Recovery

Abstract

Simultaneous saccharification and fermentation (SSF) with delayed yeast extract feeding (DYEF) was conducted in a 2-L bioreactor equipped with in-situ recovery using a gas stripping in order to enhance biobutanol production from lignocellulosic biomass of oil palm empty fruit bunch (OPEFB). This study showed that 2.88 g/L of biobutanol has been produced from SSF with a similar yield of 0.23 g/g as compared to separate hydrolysis and fermentation (SHF). An increase of 42% of biobutanol concentration was observed when DYEF was introduced in the SSF at 39 h of fermentation operation. Biobutanol production was further enhanced up to 11% with a total improvement of 72% when in-situ recovery using a gas stripping was implemented to reduce the solvents inhibition in the bioreactor. In overall, DYEF and in-situ recovery were able to enhance biobutanol production in SSF.

Bibliometric Analysis on Biobutanol Production Research Trends from 2010-2022 Using Scopus Database

“…Biobutanol has good characteristics such as less corrosive, low volatility, high heat value, high viscosity and high hydrophobicity which are comparable to gasoline, demonstrating the benefit of selecting biobutanol as the next generation of biofuels (Jiang et al 2018;Jiménez-Bonilla & Wang 2018;Khalifa et al 2018;Peabody & Kao 2016;Salleh et al 2019). This clearly depicts the fundamental reasons behind the choice of utilizing biobutanol as an alternative source of renewable energy.…”

Thermophysical Properties of 1-Butanol at High Pressures

“…However, much attention has been paid to the use of 1-butanol as a fuel. 1-Butanol can be used as an additive for gasoline, diesel, and kerosene or as an alternative fuel [1][2][3][4]. Studies show that the use of 1-butanol for this purpose is much more advantageous than the use of ethanol.…”

“…1-Butanol has higher energy density, lower than ethanol vapor pressure and improved miscibility with gasoline, lower solubility in water and it is less corrosive than ethanol [2,5,6]. Currently, 1-butanol is produced almost entirely from petroleum and the global demand for 1-butanol is about 2.8 metric tons per year [1]. The International Energy Agency forecasts that the demand for biofuels will be 690 million tons per year in 2050 [1].…”

“…Currently, 1-butanol is produced almost entirely from petroleum and the global demand for 1-butanol is about 2.8 metric tons per year [1]. The International Energy Agency forecasts that the demand for biofuels will be 690 million tons per year in 2050 [1]. The strong renewed interest in 1-butanol as a sustainable vehicle fuel has led to the development of improved biobutanol production processes including both biotechnological production processes as well as separation techniques [1,[7][8][9][10][11].…”

“…The International Energy Agency forecasts that the demand for biofuels will be 690 million tons per year in 2050 [1]. The strong renewed interest in 1-butanol as a sustainable vehicle fuel has led to the development of improved biobutanol production processes including both biotechnological production processes as well as separation techniques [1,[7][8][9][10][11]. Various species of the Clostridium bacteria such as C. acetobutylicum, C. beijerinckii, C. saccharoperbutylacetonicum and C. saccharobutylicum are mainly used for 1-butanol production [7].…”