Improved biosynthesis of 5-hydroxymethyl-2-furancarboxylic acid and furoic acid from biomass-derived furans with high substrate tolerance of recombinant Escherichia coli HMFOMUT whole-cells

Wang, Ziwei; Gong, Chunjie; He, Yiming

doi:10.1016/j.biortech.2020.122930

Cited by 48 publications

(24 citation statements)

References 24 publications

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“…A reasonable explanation was that the strategy developed in this study to enhance the catalytic performances of cells was valid and reliable, which was also evidenced by the difference between the crude activities of inducer-free cells and HMF-induced cells (Supplementary Figure 1). Compared to the microorganisms reported previously (Zhou et al, 2017;Shi et al, 2019;Cheng et al, 2020;Wang et al, 2020;Wen et al, 2020), biocatalysts of this study appeared to be much more prevailing in synthesis of FA from FAL, because of its high tolerance toward the substrate as well as good catalytic performances.…”

Section: Bioconversion Of Fal With High Loading Under Optimal Conditionsmentioning

confidence: 64%

“…However, contrarily to pure enzymes, side-reactions are inevitable for whole-cell biotransformation, which always is the most important issue to be considered (de Carvalho, 2011 ). In terms of the whole-cell bioconversion of FAL, oxidizing to FA and reducing to FOL usually occurred together in cells, and the concomitant FOL would greatly reduce the selectivity of FA (Wang et al, 2020 ). Figure 2A showed the effects of reaction pH on FA and FOL production when pH values varied from 5.5 to 7.5.…”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 94%

“…Selective oxidation of FAL and HMF to a variety of highly functionalized carboxylic acids and hydroxyl acids was an important aspect in their upgrading (Mariscal et al, 2016 ; Hu et al, 2018 ). FAL can be oxidized to furoic acid (FA) (Mariscal et al, 2016 ; Shi et al, 2019 ; Wang et al, 2020 ), while HMF to 5-hydroxymethylfuroic acid (HMFCA), 2,5-diformylfuran, 5-formylfuroic acid, and 2,5-furandicarboxylic acid (FDCA) (Qin et al, 2015 ; Hu et al, 2018 ). Up to now, chemocatalytic approaches for selective oxidation of FAL and HMF have been extensively studied, which often involve the use of toxic and hazardous reagents and solvents, the harsh conditions, as well as the low selectivity (Mariscal et al, 2016 ; Sajid et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

“…Based on catalytic promiscuity of enzymes, recombinant Escherichia coli strains harboring various heterogeneous enzymes were reported for FA production. These enzymes included 3-succinoylsemialdehyde-pyridine dehydrogenase (SAPDH) (Shi et al, 2019 ), horse liver alcohol dehydrogenase (Peng et al, 2019 ), and HMF oxidase (HMFO) variant (Wang et al, 2020 ). Likewise, microbes resistant to FAL were also isolated and implemented for FA production.…”

Section: Introductionmentioning

confidence: 99%

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Selective Biosynthesis of Furoic Acid From Furfural by Pseudomonas Putida and Identification of Molybdate Transporter Involvement in Furfural Oxidation

Zheng

Tan

et al. 2020

Front. Chem.

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Upgrading of furanic aldehydes to their corresponding furancarboxylic acids has received considerable interest recently. Herein we reported selective oxidation of furfural (FAL) to furoic acid (FA) with quantitative yield using whole-cells of Pseudomonas putida KT2440. The biocatalytic capacity could be substantially promoted through adding 5-hydroxymethylfurfural into media at the middle exponential growth phase. The reaction pH and cell dosage had notable impacts on both FA titer and selectivity. Based on the validation of key factors for FAL conversion, the capacity of P. putida KT2440 to produce FAL was substantially improved. In batch bioconversion, 170 mM FA was produced with selectivity nearly 100% in 2 h, whereas 204 mM FA was produced with selectivity above 97% in 3 h in fed-batch bioconversion. Particularly, the role of molybdate transporter in oxidation of FAL and 5-hydroxymethylfurfural was demonstrated for the first time. The furancarboxylic acids synthesis was repressed markedly by destroying molybdate transporter, which implied Mo-dependent enzyme/molybdoenzyme played pivotal role in such oxidation reactions. This research further highlights the potential of P. putida KT2440 as next generation industrial workhorse and provides a novel understanding of molybdoenzyme in oxidation of furanic aldehydes.

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Section: Bioconversion Of Fal With High Loading Under Optimal Conditionsmentioning

confidence: 64%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Selective Biosynthesis of Furoic Acid From Furfural by Pseudomonas Putida and Identification of Molybdate Transporter Involvement in Furfural Oxidation

Zheng

Tan

et al. 2020

Front. Chem.

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Recent advances in biotransformation of 5‐Hydroxymethylfurfural: challenges and future aspects

Saikia

Rathankumar

Kumar

et al. 2021

J of Chemical Tech & Biotech

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In a biorefinery process, 5-Hydroxymethylfurfural (HMF) is a promising biomass-derived chemical with diverse industrial applications. The biotransformation of HMF to different high-valued furanic derivatives, such as 2,5-furandicarboxylic acid, 2,5-diformylfuran, 5-hydroxymethyl-2-furancarboxylic acid, and 5-formyl-2-furancarboxylic acid, has led to the transition from fossil-derived products to bio-based chemicals through sustainable routes. Although, chemocatalytic conversion of HMF has been extensively studied in the recent years, the higher selectivity, environment friendliness, and mild reaction conditions of HMF bioconversion has established these processes as a promising alternative to harsh chemical conditions. To understand the present status and challenges of HMF bioconversion, this review retrospects and describes the recent advancements in the biotransformation of HMF and systematically assesses the recent findings and developments in the HMF biotransformation through various reaction schemes. Further, this review illustrates, in comprehensive detail, the merits and challenges of various biological processes and it proposes a few research trends for economical and facile HMF bioconversion for commercialization.

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Microbial Lipid Production from Lignocellulosic Biomass Pretreated by Effective Pretreatment

2021

Emerging Technologies for Biorefineries, Biofuels, and Value-Added Commodities

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Improved biosynthesis of 5-hydroxymethyl-2-furancarboxylic acid and furoic acid from biomass-derived furans with high substrate tolerance of recombinant Escherichia coli HMFOMUT whole-cells

Cited by 48 publications

References 24 publications

Selective Biosynthesis of Furoic Acid From Furfural by Pseudomonas Putida and Identification of Molybdate Transporter Involvement in Furfural Oxidation

Selective Biosynthesis of Furoic Acid From Furfural by Pseudomonas Putida and Identification of Molybdate Transporter Involvement in Furfural Oxidation

Recent advances in biotransformation of 5‐Hydroxymethylfurfural: challenges and future aspects

Microbial Lipid Production from Lignocellulosic Biomass Pretreated by Effective Pretreatment

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