Improved cellular infiltration into 3D interconnected microchannel scaffolds formed by using melt-spun sacrificial microfibers

Wang, Zongliang; Gao, Tianlin; Cui, Liguo; Wang, Yu; Zhang, Peibiao; Chen, Xuesi

doi:10.1039/c5ra25142g

Cited by 18 publications

(6 citation statements)

References 28 publications

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“…Using such bers, we have fabricated three-dimensional scaffolds for bone tissue engineering in our laboratory. [24][25][26] Mechanical properties…”

Section: Morphology and Diametersmentioning

confidence: 99%

Cotton-like micro- and nanoscale poly(lactic acid) nonwoven fibers fabricated by centrifugal melt-spinning for tissue engineering

et al. 2018

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“…Using such bers, we have fabricated three-dimensional scaffolds for bone tissue engineering in our laboratory. [24][25][26] Mechanical properties…”

Section: Morphology and Diametersmentioning

confidence: 99%

Cotton-like micro- and nanoscale poly(lactic acid) nonwoven fibers fabricated by centrifugal melt-spinning for tissue engineering

et al. 2018

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“…Material porosity in TESSs is important to allow cells to migrate and extend past the surface to form 3D cultures [ 41 , 42 ]. Studies have shown that scaffolds with 60–90% porosity are favorable for cutaneous healing applications as they provide sufficient oxygen and nutrient exchange, space for cellular activities, and the production of new ECM [ 43 , 44 ]. Adequate porosity also allows biomaterials to have sufficient nutrient transfer, molecular adsorption due to increased surface area, and mechanical properties [ 45 ].…”

Section: Resultsmentioning

confidence: 99%

Innervation of an Ultrasound-Mediated PVDF-TrFE Scaffold for Skin-Tissue Engineering

Westphal,

Bryan,

Krutko

et al. 2023

Biomimetics

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In this work, electrospun polyvinylidene-trifluoroethylene (PVDF-TrFE) was utilized for its biocompatibility, mechanics, and piezoelectric properties to promote Schwann cell (SC) elongation and sensory neuron (SN) extension. PVDF-TrFE electrospun scaffolds were characterized over a variety of electrospinning parameters (1, 2, and 3 h aligned and unaligned electrospun fibers) to determine ideal thickness, porosity, and tensile strength for use as an engineered skin tissue. PVDF-TrFE was electrically activated through mechanical deformation using low-intensity pulsed ultrasound (LIPUS) waves as a non-invasive means to trigger piezoelectric properties of the scaffold and deliver electric potential to cells. Using this therapeutic modality, neurite integration in tissue-engineered skin substitutes (TESSs) was quantified including neurite alignment, elongation, and vertical perforation into PVDF-TrFE scaffolds. Results show LIPUS stimulation promoted cell alignment on aligned scaffolds. Further, stimulation significantly increased SC elongation and SN extension separately and in coculture on aligned scaffolds but significantly decreased elongation and extension on unaligned scaffolds. This was also seen in cell perforation depth analysis into scaffolds which indicated LIPUS enhanced perforation of SCs, SNs, and cocultures on scaffolds. Taken together, this work demonstrates the immense potential for non-invasive electric stimulation of an in vitro tissue-engineered-skin model.

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“…In addition, no significant difference was noticed for cell proliferation at 7 d among all the different density fiber scaffolds. The reason might be the similar surface properties of PLA fibers and appropriate 3D structure providing enough space to fulfill cell ingrowth. , Hence, the MD fiber scaffolds bonded for 90 min was chosen for the following studies benefiting from the excellent mechanical strength, porosity, and internal fiber distribution. The cell permeability within the MD fiber scaffolds was then evaluated by cultivating cells for 7 d. From ESEM images of the cross section of the cell-scaffold construct, it was observed that a large amount of cells have been appeared on the fiber surface surrounded by the ECM even though the porosity of the MD scaffold was less than 85%, which could fully meet the needs of cell ingrowth.…”

Section: Discussionmentioning

confidence: 99%

Highly Permeable Gelatin/Poly(lactic acid) Fibrous Scaffolds with a Three-Dimensional Spatial Structure for Efficient Cell Infiltration, Mineralization and Bone Regeneration

Wang

Tang

Yakufu

et al. 2020

ACS Appl. Bio Mater.

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Three-dimensional (3D) fibrous scaffolds allowing sufficient cell infiltration are urgently needed for bone tissue engineering. In this study, a highly permeable 3D interconnected scaffold was fabricated by surface bonding of cotton-like nonwoven fibers with micro- and nanoscale architecture using gaseous chloroform. The results of physiochemical characterization indicated that bonding for 90 min with a fiber density of 0.15 g/cm3 could facilitate satisfactory porosity, supportive mechanical properties, and a 3D spatial microstructure for cell ingrowth. Coating with gelatin on the fibers induced highly efficient in vitro mineralization and in vivo bone formation as indicated by mineral deposition and repair of rabbit radius bone defect. The findings from this work demonstrated that these biofunctionalized fibrous scaffolds could bionically represent topographic nanofeatures and biological composition for cell binding affinities similar to those of the natural extracellular matrix (ECM). It can be concluded that the facile fabrication and modification strategy of 3D fibrous scaffolds exhibit promising prospect to fulfill the progressive needs in bone tissue engineering.

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Improved cellular infiltration into 3D interconnected microchannel scaffolds formed by using melt-spun sacrificial microfibers

Abstract: We report a novel fabrication method using melt-spun sacrificial microfibers to make 3D interconnected microchannel scaffolds for improved cellular infiltration.

Cited by 18 publications

References 28 publications

Cotton-like micro- and nanoscale poly(lactic acid) nonwoven fibers fabricated by centrifugal melt-spinning for tissue engineering

Cotton-like micro- and nanoscale poly(lactic acid) nonwoven fibers fabricated by centrifugal melt-spinning for tissue engineering

Innervation of an Ultrasound-Mediated PVDF-TrFE Scaffold for Skin-Tissue Engineering

Highly Permeable Gelatin/Poly(lactic acid) Fibrous Scaffolds with a Three-Dimensional Spatial Structure for Efficient Cell Infiltration, Mineralization and Bone Regeneration

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