Improved ceramic-based multisite microelectrode for rapid measurements of l-glutamate in the CNS

Burmeister, Jason J.; Pomerleau, François; Palmer, Michael R.; Day, Brian K.; Huettl, Peter; Gerhardt, Greg A.

doi:10.1016/s0165-0270(02)00172-3

Cited by 199 publications

(236 citation statements)

References 23 publications

Supporting

Mentioning

228

Contrasting

Unclassified

Order By: Relevance

“…MEAs were assembled and selected for in vivo recordings as previously described (Burmeister et al, 2002(Burmeister et al, , 2000. Preparation of the MEA for recording has been extensively described in Hascup et al, 2006 andBurmeister et al, 2002.…”

Section: Microelectrode Array Designmentioning

confidence: 99%

“…Preparation of the MEA for recording has been extensively described in Hascup et al, 2006 andBurmeister et al, 2002. Briefly, the platinum recording sites were dip coated with Nafion (Sigma-Aldrich, St Louis, MO) to repel anions (Burmeister and Gerhardt, 2001).…”

Section: Microelectrode Array Designmentioning

confidence: 99%

“…This meant the sentinel sites could not record any converted glutamate but could record any electroactive interferents (at + 0.7 V vs a Ag/AgCl reference electrode) not repelled by Nafion. When the current recorded on the sentinel sites was subtracted from the current on the glutamate recording sites, the resulting signal represents the resting glutamate levels in brain tissue (Hascup et al, 2010;Burmeister et al, 2002;Burmeister and Gerhardt, 2001). …”

Section: Microelectrode Array Designmentioning

confidence: 99%

“…For a complete review of the studies conducted in this rat model, the reader is referred to Overstreet et al, 2005. As alterations in glutamatergic neurotransmission may be an underlying cause in the pathology of depression, we decided to study resting glutamate levels in the FSL rats compared with the control FRL rats. We used an enzyme-based microelectrode array (MEA), selective for measuring glutamate with low limits of detection (o0.2 mM), fast temporal resolution (500 ms) (Burmeister et al, 2000(Burmeister et al, , 2002(Burmeister et al, , 2004Burmeister and Gerhardt, 2001) and minimal damage to surrounding brain tissue (50-100 mm) (Hascup et al, 2009). Using these MEAs in combination with pharmacological treatments, we have shown that resting glutamate levels in the medial prefrontal cortex (PFC) are at least 50% neuronally derived.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Resting Glutamate Levels and Rapid Glutamate Transients in the Prefrontal Cortex of the Flinders Sensitive Line Rat: A Genetic Rodent Model of Depression

Hascup

Stephens

et al. 2011

Neuropsychopharmacol

Self Cite

View full text Add to dashboard Cite

Despite the numerous drugs targeting biogenic amines for major depressive disorder (depression), the search for novel therapeutics continues because of their poor response rates (B30%) and slow onset of action (2-4 weeks). To better understand role of glutamate in depression, we used an enzyme-based microelectrode array (MEA) that was selective for glutamate measures with fast temporal (2 Hz) and high spatial (15 Â 333 mm) resolution. These MEAs were chronically implanted into the prefrontal cortex of 3-to 6-month-old and 12-to 15-month-old Flinders Sensitive Line (FSL) and control Flinders Resistant Line (FRL) rats, a validated genetic rodent model of depression. Although no changes in glutamate dynamics were observed between 3 and 6 months FRL and FSL rats, a significant increase in resting glutamate levels was observed in the 12-to 15-month-old FSL rats compared with the 3-to 6-month-old FSL and age-matched FRL rats on days 3-5 post-implantation. Our MEA also recorded, for the first time, a unique phenomenon in all the four rat groups of fluctuations in resting glutamate, which we have termed glutamate transients. Although these events lasted only for seconds, they did occur throughout the testing paradigm. The average concentration of these glutamate-burst events was significantly increased in the 12-to 15-month-old FSL rats compared with 3-to 6-month-old FSL and age-matched FRL rats. These studies lay the foundation for future studies of both tonic and phasic glutamate signaling in rat models of depression to better understand the potential role of glutamate signaling in depression.

show abstract

Section: Microelectrode Array Designmentioning

confidence: 99%

Section: Microelectrode Array Designmentioning

confidence: 99%

Section: Microelectrode Array Designmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Resting Glutamate Levels and Rapid Glutamate Transients in the Prefrontal Cortex of the Flinders Sensitive Line Rat: A Genetic Rodent Model of Depression

Hascup

Stephens

et al. 2011

Neuropsychopharmacol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Ceramicbased microelectrode arrays (MEAs) that contained four platinum (Pt) recording surfaces were prepared to selectively measure Glu (Figure 1a). These electrodes were fabricated for in vivo recordings using published methods (Burmeister et al, 2000(Burmeister et al, , 2002Nickell et al, 2005). All four sites were electroplated with meta-phenylenediamine (mPD) by applying a potential of + 0.5 V to the Pt sites vs a silver/silver chloride (Ag/AgCl) reference electrode (Bioanalytical Systems, RE-5) in a deoxygenated 0.05 M phosphate-buffered saline (PBS, pH 7.1-7.4) with 5.0 mM mPD.…”

Section: Microelectrode Array Preparation and In Vitro Calibrationsmentioning

confidence: 99%

Decreased Dopamine D4 Receptor Expression Increases Extracellular Glutamate and Alters Its Regulation in Mouse Striatum

Thomas

Grandy

Gerhardt

et al. 2008

Neuropsychopharmacol

Self Cite

View full text Add to dashboard Cite

To better understand the effect of the dopamine D4 receptor (DRD4) on glutamate (Glu) neurotransmission in the brain, we utilized transgenic mice with partial or complete removal of functional DRD4 plasma membrane expression (DRD4 + /À and DRD4À/À, respectively). We measured resting extracellular Glu levels, Glu clearance kinetics, and KCl-evoked release of Glu in the striatum and nucleus accumbens core of these mice using in vivo amperometry coupled to a novel microelectrode array configured for sub-second detection of Glu. Recordings from DRD4À/À and DRD4 + /À mice were compared with their wild-type littermates (DRD4 + / + ). Resting extracellular levels of Glu were increased in the striatum of DRD4À/À mice (po0.01). Glu clearance kinetics were significantly decreased in the dorsal striatum of DRD4À/À mice (po0.05). KCl-evoked overflow of Glu was reliably measured but unchanged in the striatum of the three groups. By contrast, no changes in resting Glu, Glu uptake kinetics, or KCl-evoked release of Glu were observed in the nucleus accumbens core among the three genotypes. These data indicate that the DRD4 receptor is involved in modulation of Glu neurotransmission, primarily in the striatum. A better understanding of Glu control by the DRD4 may improve our understanding of the physiological role of the DRD4 in disorders such as attention-deficit/hyperactivity disorder and schizophrenia.

show abstract

Enzyme‐Based Biosensors: Synthesis and Applications

Cao

Chen²,

Jin

et al. 2011

Biosensor Nanomaterials

View full text Add to dashboard Cite

Improved ceramic-based multisite microelectrode for rapid measurements of l-glutamate in the CNS

Cited by 199 publications

References 23 publications

Resting Glutamate Levels and Rapid Glutamate Transients in the Prefrontal Cortex of the Flinders Sensitive Line Rat: A Genetic Rodent Model of Depression

Resting Glutamate Levels and Rapid Glutamate Transients in the Prefrontal Cortex of the Flinders Sensitive Line Rat: A Genetic Rodent Model of Depression

Decreased Dopamine D4 Receptor Expression Increases Extracellular Glutamate and Alters Its Regulation in Mouse Striatum

Enzyme‐Based Biosensors: Synthesis and Applications

Contact Info

Product

Resources

About