Improved cotyledonary node method using an alternative explant derived from mature seed for efficient Agrobacterium-mediated soybean transformation

Paz, Margie M.; Martı́nez, Juan Carlos; Kalvig, Andrea B.; Fonger, Tina M.; Wang, Kan

doi:10.1007/s00299-005-0113-2

Cited by 65 publications

(80 citation statements)

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“…Complexity of Agrobacterium species and labor intensive procedure of cell-culture and difficulties associated with shoot regeneration in some plants still need to be improved for improving transformation frequency. Among factors, Sonication-Assisted Agrobacterium-mediated Transformation (SAAT) (Joersbo and Brunstedt 1992;Trick and Finer 1998;Santarem et al 1998) and vacuuminfiltration (Charity et al 2002;Park et al 2005;Paz et al 2006) methods have been reported to enhance the efficiency of Agrobacterium-mediated transformation of plant species. Hence in the present study, we have tested sonication and vacuum-infiltration of explants to study their influence on transgenic efficiency.…”

Section: Introductionmentioning

confidence: 99%

Agrobacterium-mediated transformation in chickpea (Cicer arietinum L.) with an insecticidal protein gene: optimisation of different factors

Indurker

Misra

Eapen

2010

Physiol Mol Biol Plants

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Agrobacterium-mediated transformation in chickpea was developed using strain LBA4404 carrying nptII, uidA and cryIAc genes and transformants selected on Murashige and Skoog's basal medium supplemented with benzyladenine, kinetin and kanamycin. Integration of transgenes was demonstrated using polymerase chain reaction and Southern blot hybridization of T 0 plants. The expression of CryIAc delta endotoxin and GUS enzyme was shown by enzyme linked immunosorbent assay and histochemical assay respectively. The transgenic plants (T 0 ) showed more tolerance to infection by Helicoverpa armigera compared to control plants. Various factors such as explant source, cultivar type, different preculture treatment period of explants, co-cultivation period, acetosyringone supplementation, Agrobacterium harboring different plasmids, vacuum infiltration and sonication treatment were tested to study the influence on transformation frequency. The results indicated that use of epicotyl as explant, cultivar ICCC37, Agrobacterium harboring plasmid pHS102 as vector, preculture of explant for 48 h, cocultivation period of 2 days at 25°C and vacuum infiltration for 15 min produced the best transformation results. Sonication treatment of explants with Agrobacteria for 80 s was found to increase the frequency of transformation.

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Section: Introductionmentioning

confidence: 99%

Agrobacterium-mediated transformation in chickpea (Cicer arietinum L.) with an insecticidal protein gene: optimisation of different factors

Indurker

Misra

Eapen

2010

Physiol Mol Biol Plants

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“…To improve the production of successful soybean transgenic plants, previously reported half-seed method (Paz et al 2006) with modification was used in this experiment. We have proven the efficiency of transformation with dipping with high concentration of Agrobacterium solution, sonication and vacuum during the inoculation procedure (Kim et al 2012;Kim et al 2013;Kim et al 2016).…”

Section: Discussionmentioning

confidence: 99%

“…(Gelvin 2005;Phang et al 2008;He et al 2010;Zhang et al 2010;Bhuiyan et al 2011;Li et al 2011;Subramanyam et al 2011;Movahedi et al 2012;Yarra et al 2012). Soybean transgenic plants with multiple functions have been developed using Agrobacterium-mediated transformation system based on the cotyledonary-node (CN) method (Hinchee et al 1988) and improved by using half-seed explants (Paz et al 2006). The trials with additional treatments including the mixture of thiol compounds, sonication and vacuum have elevated transformation efficiency to much higher level (Meurer et al 1998;Olhoft et al 2003;Kim et al 2012;Kim et al 2013;Kim et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Overexpression of AtSZF2 from Arabidopsis Showed Enhanced Tolerance to Salt Stress in Soybean

Kim

Pak

et al. 2017

Plant Breed. Biotech.

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Plants have adapted to environmental challenges by expressing many plant genes in response to the stresses. Among those genes, CCCH zinc finger proteins are involved in abiotic and biotic stresses. Transgenic soybean plants overexpressing AtSZF2 were produced to investigate that its ectopic overexpression enhanced salt stress tolerance by Agrobacterium-mediated transformation using half-seed explants. Sixteen transgenic lines were chosen to analyze for T-DNA insertion and transcription levels, and most of them were confirmed as positive. In further analysis with Southern blot, stable transformation event and copy number were confirmed. Following high salinity stress on the detached leaf and whole plant of two transgenic lines (#4 and #6) revealed that the ectopic expression of AtSZF2 was correlated with stress tolerance in phenotype, ion leakage and chlorophyll content with statistical significance. In another test with 20% PEG treatment, similar tolerance of transgenic plants was observed with lower ion leakage and higher chlorophyll content, indicating that the damage of cell membrane was prevented in transgenic plants. Finally, expression of various abiotic stress-responding genes was detected by reverse transcriptase and quantitative real-time PCR analysis with the transgenic plants. It could be proposed that introduction of AtSZF2 resulted in the modulation of ABA/stress responsive gene expression in transgenic soybean plants and make them tolerant against salt stress. Considering soybean as a salt-sensitive crop and importance of salt stress tolerance in specific farming region, the introduction of AtSZF2 may provide an approach for crop improvement in soybean breeding.

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“…Therefore, improvement of soybean characteristics via regeneration and transformation techniques is of critical importance. Although soybean is one of the most recalcitrant crops (Hofmann et al, 2004;Khalafalla et al, 2006;Paz et al, 2006), many soybean regeneration and transformation methods have been established over the years. However, the efficiency of these techniques is considerably restricted by genotype-dependence (Paz et al, 2004;Reichert et al, 2003;Sairam et al, 2003;Yang et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Mapping QTLs for Tissue Culture Response in Soybean (Glycine max (L.) Merr.)

Yang

Zhao

et al. 2011

Molecules and Cells

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Quantitative trait loci (QTLs) that control the tissue culture response in soybean were detected by using 184 recombinant inbred lines (RILs) derived from two varieties: Kefeng No.1 and Nannong 1138-2. The molecular map consisting of 834 molecular markers using this population covered space 2307.83 cM of the genome throughout 24 linkage groups. The performance of tissue culture in soybean was evaluated by two indices: callus induction frequency (CIF) and somatic embryos initiation frequency (SEIF). They were expressed as the number of explants producing callus/ the number of total explants and the number of explants producing somatic embryos/ the number of total explants, respectively. The RIL lines showed continuous segregation for both indices. With the composite interval mapping (CIM) described in Windows QTL Cartographer Version 2.5, three quantitative trait loci (QTLs) were identified for the frequency of callus induction, on chromosomes B2 and D2, accounting for phenotypic variation from 5.84% to 16.60%; four QTLs on chromosome G were detected for the frequency of somatic embryos initiation and explained the phenotypic variation from 7.79% to 14.16%. The information of new QTLs identified in the present study will contribute to genetic improvement of regeneration traits with marker-assisted selection (MAS) in soybean.

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Improved cotyledonary node method using an alternative explant derived from mature seed for efficient Agrobacterium-mediated soybean transformation

Cited by 65 publications

References 0 publications

Agrobacterium-mediated transformation in chickpea (Cicer arietinum L.) with an insecticidal protein gene: optimisation of different factors

Agrobacterium-mediated transformation in chickpea (Cicer arietinum L.) with an insecticidal protein gene: optimisation of different factors

Overexpression of AtSZF2 from Arabidopsis Showed Enhanced Tolerance to Salt Stress in Soybean

Mapping QTLs for Tissue Culture Response in Soybean (Glycine max (L.) Merr.)

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