Improved Detection of Immunoglobulin M in Sera of Erythema Migrans Patients by Western Blotting with a Local Borrelia Burgdorferi Skin Isolate

Detection ofBorrelia burgdorferi sensu stricto by reverse line blot in the joints of Dutch patients with Lyme arthritis

“…Anti-B burgdorferi antibodies were detected in serum using full-antigen enzyme-linked immunosorbent assay (ELISA; Dako, Glostrup, Denmark) and immunoblotting for IgM and IgG (34,35). Only those patients who were seropositive by ELISA and showed a specific immunoblot pattern compatible with a B burgdorferi sensu lato infection by immunoblotting, thus fulfilling the criteria for early Lyme disease proposed by Engstrom et al (36), were considered positive.…”