Improved detection ofPneumocystis carinii by an immunofluorescence technique using monoclonal antibodies

Orholm, Marianne; Holten-Andersen, Winnie; Lundgren, Jens D.

doi:10.1007/bf01967503

Cited by 9 publications

(8 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…27), whereas MAbs raised against cells from rats may stain P. cariniiderived from both human and animal sources (Kovacs et al 1989). Immunostaining of P. carinii, especially in bronchoalveolar lavage fluid, has been a valuable adjunct to diagnostic efforts in patients with severe T-cell deficiency (Orholm et d. 1990).…”

Section: Pneumocystis Carinii and Algaementioning

confidence: 99%

Diagnosis of systemic mycoses by specific immunohistochemical tests

Jensen¹,

Schønheyder

Hotchi

et al. 1996

Apmis

View full text Add to dashboard Cite

of systemic mycoses by specific immunohistochemical tests. APMIS 104, 241-258, 1996. Immunohistochemistry has proved to be a powerful tool for the accurate diagnosis of a number of important mycoses in humans and animals, such as aspergillosis, candidosis, cryptococcosis, blastomycosis, coccidioidomycosis, histoplasmosis capsulati and duboisii, paracoccidioidomycosis, fusariosis, pseudallescheriosis (scedosporiosis), sporotrichosis, trichosporonosis, penicilliosis, and zygomycosis (mucormycosis). These techniques are also applicable to pneumocystosis and to non-mycotic infections caused by algae such as protothecosis. Apart from the specificity of immunohistochemistry, the application of fluorochromes is highly effective for the localization of typical or atypical fungal elements in lesions with only few organisms present. Occasionally, a dual aetiology of fungal infections may be suspected on the basis of morphological study, and dual staining techniques have the capacity for resolving this question by simultaneous and differential staining of two fungal species present in a tissue specimen.

show abstract

Section: Pneumocystis Carinii and Algaementioning

confidence: 99%

Diagnosis of systemic mycoses by specific immunohistochemical tests

Jensen¹,

Schønheyder

Hotchi

et al. 1996

Apmis

View full text Add to dashboard Cite

show abstract

“…Cysts or trophozoites are morphologically identified by methenamine-silver nitrate or giemsa stains respectively. Immunospecific stains are now available and have increased the sensitivity of detection in sputum and bronchoalveolar lavage fluid [24]. The number of organisms in diagnostic specimens is high in HIV positive patients, but often low in HIV negative cases.…”

Section: Introductionmentioning

confidence: 99%

Autoimmune inflammatory disorders, systemic corticosteroids and pneumocystis pneumonia: A strategy for prevention

Sowden

Carmichael

2004

BMC Infect Dis

View full text Add to dashboard Cite

Background: Pneumocystis pneumonia (PCP) is an increasing problem amongst patients on immunosuppression with autoimmune inflammatory disorders (AID). The disease presents acutely and its diagnosis requires bronchoalveolar lavage in most cases. Despite treatment with intravenous antibiotics, PCP carries a worse prognosis in AID patients than HIV positive patients. The overall incidence of PCP in patients with AID remains low, although patients with Wegener's granulomatosis are at particular risk.

show abstract

“…Sputum induction was performed as described (7). For PCR analysis, 5 ml of sputum was treated with 3.25 mM dithiothreitol (Sputolysin, Behring Diagnostic, Somerville, New Jersey, USA) for 5 min at 37"C, and cells were spun down 500 x G, for 10 min, washed in PBS (SSI, Copenhagen, Denmark) and spun down again.…”

Section: Methodsmentioning

confidence: 99%

Pneumocystis carinii in Bronchoalveolar Lavage and Induced Sputum: Detection with a Nested Polymerase Chain Reaction

Skøt

Lerche

Kolmos

et al. 1995

Scandinavian Journal of Infectious Diseases

View full text Add to dashboard Cite

To evaluate polymerase chain reaction (PCR) for detection of Pneumocystis carinii, 117 bronchoalveolar lavage (BAL) specimens, from HIV-infected patients undergoing a diagnostic bronchoscopy, were processed and a nested PCR, followed by Southern blot and hybridization with a P32-labelled probe was performed. The sensitivity and specificity were 85 and 100% 934/40 and 77/77) respectively. A non-radioactive labelling system BluGENE was evaluated on all specimens, and found to be as effective as P32-labelling. To increase the speed and convenience of detection, a dot blot system was tested, but sensitivity dropped markedly with this system. A further 33 patients had both induced sputum and bronchoalveolar lavage performed and the induced sputum was analysed using PCR and routine microbiological methods. The PCR sensitivity on induced sputum was equal to that of routine methods. At present the evaluated PCR cannot replace routine microbiological methods for detection of Pneumocystis carinii, on either BAL fluid or induced sputum.

show abstract

Improved detection ofPneumocystis carinii by an immunofluorescence technique using monoclonal antibodies

Cited by 9 publications

References 19 publications

Diagnosis of systemic mycoses by specific immunohistochemical tests

Diagnosis of systemic mycoses by specific immunohistochemical tests

Autoimmune inflammatory disorders, systemic corticosteroids and pneumocystis pneumonia: A strategy for prevention

Pneumocystis carinii in Bronchoalveolar Lavage and Induced Sputum: Detection with a Nested Polymerase Chain Reaction

Contact Info

Product

Resources

About