Improved determination of 2-dodecylcyclobutanone in irradiated ground beef patties by gas-chromatography–mass-spectrometry (GC/MS) coupled with solid-phase microextraction (SPME) technique

Soncin, S.; Panseri, Sara; Rusconi, Michele; Mariani, Mario; Chiesa, Luca Maria; Biondi, Pier Antonio

doi:10.1016/j.foodchem.2012.02.089

Cited by 17 publications

(18 citation statements)

References 19 publications

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“…This result agrees with similar studies on the effect of the irradiation dose on the 2-DCB concentration. 3,7,8,19,20 In summary, the immunogen and the coating antigen for 2-DCB were successfully prepared and the monoclonal antibody against 2-DCB was likewise prepared using a hybridoma technique. A highly sensitive and specific ciELISA method for the detection of 2-DCB in irradiated ground beef patties was developed.…”

Section: Synthesis Of Immunogen and Coating Antigenmentioning

confidence: 99%

Development of a Competitive Indirect Enzyme-Linked Immunosorbent Assay Based on Monoclonal Antibodies for the Detection of 2-Dodecylcyclobutanone in Irradiated Beef

Zhao

Wang

et al. 2013

J. Agric. Food Chem.

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A highly sensitive and specific competitive indirect enzyme-linked immunosorbent assay (ciELISA) based on monoclonal antibodies was developed for the detection of 2-dodecylcyclobutanone (2-DCB), a chemical marker for irradiated lipid-containing foods. 2-Oxocyclobutane undecanoic acid was used as an alternative to 2-DCB and conjugated to BSA and OVA via a conventional carbodiimide condensation reaction to prepare the immunogen and the coating antigen for 2-DCB. The monoclonal antibody against 2-DCB was obtained using the hybridoma technique, with a high specificity and low cross-reactivity for 2-tetradecylcyclobutanone (2-TCB; <8%) and other structurally related compounds (<0.1%). The ciELISA method was applicable at optimal experimental conditions of 0.001-100 μg/mL 2-DCB in a buffer solution, with an IC50 value of 0.25 μg/mL and a limit of detection (defined as the IC20) of approximately 0.004 μg/mL. The recovery efficiency of 2-DCB from ground beef patties ranged from 84.4 to 109.8%. The intra-assay and interassay coefficients of variation were <10.0 and <12.0%, respectively. The proposed method was validated by gas chromatography-mass spectrometry with high correlation. The same method was used to detect 2-DCB in ground beef patties irradiated at 0.5, 1.0, 3.0, 5.0, and 7.0 kGy; the 2-DCB concentration linearly increased with the radiation dose.

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Section: Synthesis Of Immunogen and Coating Antigenmentioning

confidence: 99%

Development of a Competitive Indirect Enzyme-Linked Immunosorbent Assay Based on Monoclonal Antibodies for the Detection of 2-Dodecylcyclobutanone in Irradiated Beef

Zhao

Wang

et al. 2013

J. Agric. Food Chem.

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“…This procedure shows different critical points, such as long and solventconsuming extraction and a complex clean-up step (Crews et al, 2012). For this reason, the European Standard EN 1785:2003 method is unsuitable for routine analysis; therefore, advanced analytical methods to International Journal of Food Science & Technology 2020 isolate and detect 2-ACBs have been developed (Gadgil et al, 2002;Obana et al, 2005;Soncin et al, 2012;Breidbach & Ulberth, 2016;Li et al, 2017;).…”

Section: Introductionmentioning

confidence: 99%

Validation of an alternative method for the identification of 2‐dodecylcyclebutanone (2‐DCB) of irradiated meats by solid‐phase microextraction (SPME) gas chromatography–mass spectrometry (GC‐MS)

Campaniello

Marchesani

Zianni

et al. 2019

Int J of Food Sci Tech

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Summary A rapid and simple procedure to detect irradiated food containing fat is proposed. This method is based on HS‐SPME coupled with GC‐MS to determine the presence of a radiolytic product in irradiated meats: 2‐dodecylcyclobutanone (2‐DCB) and is proposed as an alternative to EN 1785:2003, which is a long and complex procedure. The qualitative confirmation method is validated on different type of meats: chicken, turkey, duck, beef, pork on both nonirradiated and irradiated samples at different dose levels (0.05, 0.12, 0.5, 1.0 and 3.0 kGy) with a biological X‐ray irradiator. The validation parameters investigated are selectivity, minimum dose level (MDL), limit of detection (LOD), sensitivity and specificity. The MDL and LOD values were 0.5 kGy and 5.0 ng mL−1, respectively, for all matrices. No false positives or false negatives occurred, and 100% of samples were correctly identified. The results show that HS‐SPME GC‐MS is suitable for routine analysis.

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“…The European official method (EN1785:2003), which is based on gas chromatography-mass spectrometry (GC-MS) detection of 2-alkylcyclobutanones (2-ACBs) from lipid radiolysis, has been widely used to identify a variety of irradiated lipidcontaining food products [12][13][14][15][16][17][18][19][20][21]. In particular, 2-d o d e c y l c y c l o b u t a n o n e ( 2 -D C B , F i g .…”

Section: Introductionmentioning

confidence: 99%

Determination of 2-alkylcyclobutanones by combining precolumn derivatization with 1-naphthalenyl hydrazine and ultra-performance liquid chromatography with fluorescence detection

et al. 2016

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2-Alkylcyclobutanones (2-ACBs) are uniquely formed when triglycerides-containing food products are exposed to ionizing radiation. Thus, 2-ACBs have been used as marker molecules to identify irradiated food. Most methods to determine 2-ACBs involve mass spectrometric detection after chromatographic separation. The spectrofluorometer is rarely used to determine 2-ACBs because these molecules do not fluoresce. In this study, we developed an ultra-performance liquid chromatography (UPLC) method to determine 2-ACBs. 2-ACBs were converted into fluorophores after reacting with 1-naphthalenyl hydrazine to facilitate their sensitive and selective detection using a fluorescence detector (FLD). Analysis of 2-ACBs using our developed UPLC-FLD method allows sensitive determination of 2-ACBs at a detection limit of 2 ng 2-ACBs per g of fat (30 pg/injection), which is significantly lower than that of existing analytical methods. After validation for trueness and precision, the method was applied to γ-irradiated chicken samples to determine their 2-ACB content. Comparative studies employing liquid chromatography-tandem mass spectrometric method revealed no systematic difference between the two methods, thereby demonstrating that the proposed UPLC-FLD method can be suitably used to determine 2-ACBs in irradiated foodstuffs. Graphical Abstract Determination of radiation-induced food-borne 2-dodecylcyclobutanone and 2-tetradecylcyclobutanone by combining 1-naphthalenyl hydrazine derivatization and ultra-performance liquid chromatography with fluorescence detection.

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Improved determination of 2-dodecylcyclobutanone in irradiated ground beef patties by gas-chromatography–mass-spectrometry (GC/MS) coupled with solid-phase microextraction (SPME) technique

Cited by 17 publications

References 19 publications

Development of a Competitive Indirect Enzyme-Linked Immunosorbent Assay Based on Monoclonal Antibodies for the Detection of 2-Dodecylcyclobutanone in Irradiated Beef

Development of a Competitive Indirect Enzyme-Linked Immunosorbent Assay Based on Monoclonal Antibodies for the Detection of 2-Dodecylcyclobutanone in Irradiated Beef

Validation of an alternative method for the identification of 2‐dodecylcyclebutanone (2‐DCB) of irradiated meats by solid‐phase microextraction (SPME) gas chromatography–mass spectrometry (GC‐MS)

Determination of 2-alkylcyclobutanones by combining precolumn derivatization with 1-naphthalenyl hydrazine and ultra-performance liquid chromatography with fluorescence detection

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