Improved determination of taurine by high-performance anion-exchange chromatography with integrated pulsed amperometric detection (HPAEC-IPAD)

Cataldi, Tommaso R. I.; Telesca, Giovanni

doi:10.1007/s00216-003-2388-x

Cited by 24 publications

(1 citation statement)

References 36 publications

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“…Structurally (Scheme 1), homotaurine is a naturally occurring small amino-sulfonate compound that shares structural similarities with the amino acid taurine. The quantification and chromatographic separation of taurine have been extensively studied using gas chromatography [8,9], ion chromatography [10], capillary electrophoresis [11] and HPLC [12]. Similarly, homotaurine has been identified as an impurity of the calcium acamprosate via electrophoresis [13], and its concentration in blood has been determined using HPLC-ELSD (Evaporative Light Scattering Detection) [14].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Permeability Study of Homotaurine Using a High-Performance Liquid Chromatography with Fluorescence Detection Pre-Column Derivatization Method

Ntorkou,

Tsanaktsidou,

Chachlioutaki

et al. 2023

Molecules

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Homotaurine (HOM) is considered a promising drug for the treatment of Alzheimer’s and other neurodegenerative diseases. In the present work, a new high-performance liquid chromatography with fluorescence detection (HPLC–FLD) (λex. = 340 nm and λem. = 455 nm) method was developed and validated for the study of substance permeability in the central nervous system (CNS). Analysis was performed on a RP-C18 column with a binary gradient elution system consisting of methanol–potassium phosphate buffer solution (pH = 7.0, 0.02 M) as mobile phase. Samples of homotaurine and histidine (internal standard) were initially derivatized with ortho-phthalaldehyde (OPA) (0.01 M), N-acetylcysteine (0.01 M) and borate buffer (pH = 10.5; 0.05 M). To ensure the stability and efficiency of the reaction, the presence of different nucleophilic reagents, namely (a) 2-mercaptoethanol (2-ME), (b) N-acetylcysteine (NAC), (c) tiopronin (Thiola), (d) 3-mercaptopropionic acid (3-MPA) and (e) captopril, was investigated. The method was validated (R2 = 0.9999, intra-day repeatability %RSD < 3.22%, inter-day precision %RSD = 1.83%, limits of detection 5.75 ng/mL and limits of quantification 17.43 ng/mL, recovery of five different concentrations 99.75–101.58%) and successfully applied to investigate the in vitro permeability of homotaurine using Franz diffusion cells. The apparent permeability (Papp) of HOM was compared with that of memantine, which is considered a potential therapeutic drug for various CNSs. Our study demonstrates that homotaurine exhibits superior permeability through the simulated blood–brain barrier compared to memantine, offering promising insights for enhanced drug delivery strategies targeting neurological conditions.

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Section: Introductionmentioning

confidence: 99%

In Vitro Permeability Study of Homotaurine Using a High-Performance Liquid Chromatography with Fluorescence Detection Pre-Column Derivatization Method

Ntorkou,

Tsanaktsidou,

Chachlioutaki

et al. 2023

Molecules

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Electrochemical and piezoelectric monitoring of taurine via electropolymerized molecularly imprinted films

Singh

2017

J of Molecular Recognition

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A molecularly imprinted electrochemical quartz crystal microbalance (EQCM) sensor is fabricated here for taurine, a β-amino acid significant for functioning of almost all vital organs. The polymeric film of l-methionine was electrochemically deposited on gold-coated EQCM electrode. Experimental parameters were optimized for controlling the performance of molecularly imprinted polymer (MIP)-modified sensor such as ratio of monomer and template, number of electropolymerization cycles, mass deposited in each cycle, and pH. Thus, fabricated MIP-EQCM sensor was successfully applied for estimation of taurine in solutions with varying matrices, such as aqueous, human blood plasma, milk from cow, buffalo, and milk powder. Under optimized parameters, response of MIP sensor to taurine was linearly proportional to its concentration with limit of detection as 0.12μM. Hence, a highly sensitive and selective piezoelectric sensor for taurine has been reported here via imprinting approach.

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Sensitive monitoring of taurine biomarker in unprocessed human plasma samples using a novel nanocomposite based on poly(aspartic acid) functionalized by graphene quantum dots

Shadjou

Alizadeh

Hasanzadeh

2018

J of Molecular Recognition

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The rapid and accurate determination of the level of taurine biomarker in various tissues and body fluids can be of great interest in the early diagnosis of several important pathologies and diseases. For the first time, this study reports on the electropolymerization of a low toxic and biocompatible nanocomposite "poly(aspartic acid)-graphene quantum dots (GQDs)" as a novel strategy for surface modification of glassy carbon electrode and preparation a new interface for measurement of taurine. Electrochemical deposition, as a well-controlled synthesis procedure, has been used for subsequently layer-by-layer preparation of GQDs nanostructures on poly(aspartic acid) using cyclic voltammetry techniques in the regime of -1.5 to 2 V. The field emission scanning electron microscopy indicated immobilization of uniformly GQDs onto poly(aspartic acid) film. The modified electrode appeared as an effective electroactivity for detection of taurine biomarker using cyclic voltammetry, chronoamperometry, and differential pulse voltammetry. Enhancement of peak currents is ascribed to the fast heterogeneous electron transfer kinetics that arise from the synergistic coupling between the excellent properties of poly(aspartic acid) as semiconducting polymer, GQDs as high density of edge plane sites, and subtle electronic characteristics to chemical modification. Under the optimized analysis conditions, the prepared sensor for detection of taurine showed a low limit of quantification 0.001mM. Finally, the resulting prepared sensor allow the quantification of these biomarkers directly in biological samples without need of derivatization schemes or sample pretreatment.

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Improved determination of taurine by high-performance anion-exchange chromatography with integrated pulsed amperometric detection (HPAEC-IPAD)

Cited by 24 publications

References 36 publications

In Vitro Permeability Study of Homotaurine Using a High-Performance Liquid Chromatography with Fluorescence Detection Pre-Column Derivatization Method

In Vitro Permeability Study of Homotaurine Using a High-Performance Liquid Chromatography with Fluorescence Detection Pre-Column Derivatization Method

Electrochemical and piezoelectric monitoring of taurine via electropolymerized molecularly imprinted films

Sensitive monitoring of taurine biomarker in unprocessed human plasma samples using a novel nanocomposite based on poly(aspartic acid) functionalized by graphene quantum dots

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